(±)-Blebbistatin, inhibitor of myosin II
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(42 Publications)
Blebbistatin (CAS: 674289-55-5)(ab120425) is a cell-permeable, reversible inhibitor of non-muscle myosin II. Shows high affinity and selectivity for vertebrate and invertebrate Myosin II (IC50 values 0.5-5 µM). MW 292.3.
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- Proven performance: cited in over 40 publications
View Alternative Names
2400004E04Rik, AI413597, AI838772, AW045860, AW493413, BDPLT 6, Cellular myosin heavy chain, Cellular myosin heavy chain type A, Cellular myosin heavy chain type B, Cellular myosin heavy chain type B type B, DDPAC, DFNA 17, DFNA4, DKFZp667A1311, EPSTS, FLJ11090, FLJ13881, FLJ31424, FLJ43092, FP17425, FTDP 17, FTNS, G protein beta1/gamma2 subunit interacting factor 1, Hypothetical protein FLJ35932, II C, KIAA2034, MAPT, MAPTL, MGC104252, MGC104539, MGC112732, MGC134287, MGC134913, MGC134914, MGC138549, MGC156663, MHA, MHC16, MSTD, MTBT1, MTBT2, MYH 2A, MYH10_HUMAN, MYH14_HUMAN, MYH1_HUMAN, MYH6_HUMAN, MYH9_HUMAN, MYHC, MYHCA, MYHCB, MYHSA 2, MYHas8, MYL2, Microtubule associated protein tau isoform 4, Microtubule-associated protein tau, Mtapt, MyHC 2A, MyHC IIa, MyHC-2x, MyHC-IIx/d, MyHC-alpha, MyHC-beta, Myosin, Myosin light chain 2 regulatory cardiac slow, Myosin II, Myosin heavy chain, Myosin heavy chain 1, Myosin heavy chain 1 skeletal muscle adult, Myosin heavy chain 10, Myosin heavy chain 10 non muscle, Myosin heavy chain 14, Myosin heavy chain 2 skeletal muscle adult, Myosin heavy chain 2x, Myosin heavy chain 3 skeletal muscle adult, Myosin heavy chain 4 skeletal muscle adult, Myosin heavy chain 6, Myosin heavy chain 6 skeletal muscle adult, Myosin heavy chain 9, Myosin heavy chain 9 non muscle, Myosin heavy chain IIx/d, Myosin heavy chain cardiac muscle alpha isoform, Myosin heavy chain cardiac muscle beta isoform, Myosin heavy chain non muscle IIa, Myosin heavy chain non muscle IIb, Myosin heavy chain non muscle IIc, Myosin heavy chain nonmuscle type B, Myosin heavy chain skeletal muscle adult 1, Myosin heavy polypeptide 10 non muscle, Myosin heavy polypeptide 14, Myosin heavy polypeptide 2, Myosin heavy polypeptide 9 non muscle, Myosin light chain 1 alkali skeletal fast, Myosin light chain 3 alkali ventricular skeletal slow, Myosin light chain 4 alkali atrial embryonic, Myosin light chain 5 regulatory, Myosin light chain 6 alkali smooth muscle and non muscle, Myosin-1, Myosin-10, Myosin-14, Myosin-6, Myosin-9, NMHC II A, NMHC II-C, NMMHC II-a, NMMHC II-b, NMMHC-A, NMMHC-B, Near to the ATP binding region, Neurofibrillary tangle protein, Non muscle myosin heavy chain, Non muscle myosin heavy chain II A, Non muscle myosin heavy polypeptide 9, Non-muscle myosin heavy chain A, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain IIa, Non-muscle myosin heavy chain IIb, Non-muscle myosin heavy chain IIc, Nonmuscle myosin II heavy chain B, Nonmuscle myosin heavy chain II A, OTTMUSP00000019210, PHF-tau, PPND, PPP1R103, Paired helical filament-tau, Protein phosphatase 1, regulatory subunit 103, RNPTAU, RP24-311F12.2, SCAN1, STK33_HUMAN, Serine threonine kinase 33, Serine/threonine kinase 33, Serine/threonine-protein kinase 33, TAU_HUMAN, TYDP, TYDP1_HUMAN, Tauopathy and respiratory failure, Tauopathy and respiratory failure, included, Tyr-DNA phosphodiesterase 1, Tyrosyl-DNA phosphodiesterase 1, adult 1, cardiac muscle alpha isoform, non-muscle IIa, non-muscle IIb, non-muscle IIc, pTau, type A, type B
- Chemical Structure
Lab
Chemical Structure - (±)-Blebbistatin, inhibitor of myosin II (AB120425)
2D chemical structure image of ab120425, Blebbistatin, inhibitor of myosin II
- FuncS
Unknown
Functional Studies - (±)-Blebbistatin, inhibitor of myosin II (AB120425)
ab32084 staining paxillin in MEF1 cells treated with (+/-)-blebbistatin (ab120425), by ICC/IF. Decreased membrane expression of paxillin correlates with increased concentration of (+/-)-blebbistatin, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120425 ((+/-)-blebbistatin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32084 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Pathways
Myosin and its variants participate prominently in the actin cytoskeleton signaling pathway. This pathway orchestrates cellular movement and structure important for proliferation and morphogenesis. Myosin's role involves coordinated function with proteins like actin itself tropomyosin and myosin light chains facilitating intracellular transport mechanisms. Additionally myosin II has interactions within the Rho GTPase signaling pathway contributing further to regulation of the actin cytoskeleton and influencing processes like cell migration and focal adhesion dynamics.
Biological function summary
These myosins assume pivotal roles in maintaining cell structure and function. They contribute significantly to cellular adhesion migration and division by interfacing systematically within the cytoskeleton. In contractile cells myosin works as part of a motor protein complex yet in non-muscle cells its responsibilities extend to cell shape maintenance and cytokinesis. These proteins directly influence cellular signaling dynamics and mechanotransduction. The non-muscle myosin IIA for example is critical for maintaining epithelial cell tight junctions emphasizing the structural diversity and importance of myosins across cell types.
Publications (42)
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Journal of the Association for Research in Otolaryngology : JARO 26:225-238 PubMed40240732
2025
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Nature neuroscience 28:49-61 PubMed39623218
2024
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Theranostics 14:6625-6651 PubMed39479451
2024
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Nature communications 15:7996 PubMed39266508
2024
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The Journal of cell biology 223: PubMed38713825
2024
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Cell stem cell 31:640-656.e8 PubMed38701758
2024
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American journal of physiology. Heart and circulatory physiology 326:H203-H215 PubMed37975708
2023
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Nature communications 14:6226 PubMed37803005
2023
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Proceedings of the National Academy of Sciences of the United States of America 120:e2308941120 PubMed37782785
2023
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Nature communications 14:5166 PubMed37620390
2023
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