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AB120425

(±)-Blebbistatin, inhibitor of myosin II

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(42 Publications)

Blebbistatin (CAS: 674289-55-5)(ab120425) is a cell-permeable, reversible inhibitor of non-muscle myosin II. Shows high affinity and selectivity for vertebrate and invertebrate Myosin II (IC50 values 0.5-5 µM). MW 292.3.

- Available in different sizes to fit your experimental needs. Larger volume format available upon request.
- Proven performance: cited in over 40 publications

View Alternative Names

2400004E04Rik, AI413597, AI838772, AW045860, AW493413, BDPLT 6, Cellular myosin heavy chain, Cellular myosin heavy chain type A, Cellular myosin heavy chain type B, Cellular myosin heavy chain type B type B, DDPAC, DFNA 17, DFNA4, DKFZp667A1311, EPSTS, FLJ11090, FLJ13881, FLJ31424, FLJ43092, FP17425, FTDP 17, FTNS, G protein beta1/gamma2 subunit interacting factor 1, Hypothetical protein FLJ35932, II C, KIAA2034, MAPT, MAPTL, MGC104252, MGC104539, MGC112732, MGC134287, MGC134913, MGC134914, MGC138549, MGC156663, MHA, MHC16, MSTD, MTBT1, MTBT2, MYH 2A, MYH10_HUMAN, MYH14_HUMAN, MYH1_HUMAN, MYH6_HUMAN, MYH9_HUMAN, MYHC, MYHCA, MYHCB, MYHSA 2, MYHas8, MYL2, Microtubule associated protein tau isoform 4, Microtubule-associated protein tau, Mtapt, MyHC 2A, MyHC IIa, MyHC-2x, MyHC-IIx/d, MyHC-alpha, MyHC-beta, Myosin, Myosin light chain 2 regulatory cardiac slow, Myosin II, Myosin heavy chain, Myosin heavy chain 1, Myosin heavy chain 1 skeletal muscle adult, Myosin heavy chain 10, Myosin heavy chain 10 non muscle, Myosin heavy chain 14, Myosin heavy chain 2 skeletal muscle adult, Myosin heavy chain 2x, Myosin heavy chain 3 skeletal muscle adult, Myosin heavy chain 4 skeletal muscle adult, Myosin heavy chain 6, Myosin heavy chain 6 skeletal muscle adult, Myosin heavy chain 9, Myosin heavy chain 9 non muscle, Myosin heavy chain IIx/d, Myosin heavy chain cardiac muscle alpha isoform, Myosin heavy chain cardiac muscle beta isoform, Myosin heavy chain non muscle IIa, Myosin heavy chain non muscle IIb, Myosin heavy chain non muscle IIc, Myosin heavy chain nonmuscle type B, Myosin heavy chain skeletal muscle adult 1, Myosin heavy polypeptide 10 non muscle, Myosin heavy polypeptide 14, Myosin heavy polypeptide 2, Myosin heavy polypeptide 9 non muscle, Myosin light chain 1 alkali skeletal fast, Myosin light chain 3 alkali ventricular skeletal slow, Myosin light chain 4 alkali atrial embryonic, Myosin light chain 5 regulatory, Myosin light chain 6 alkali smooth muscle and non muscle, Myosin-1, Myosin-10, Myosin-14, Myosin-6, Myosin-9, NMHC II A, NMHC II-C, NMMHC II-a, NMMHC II-b, NMMHC-A, NMMHC-B, Near to the ATP binding region, Neurofibrillary tangle protein, Non muscle myosin heavy chain, Non muscle myosin heavy chain II A, Non muscle myosin heavy polypeptide 9, Non-muscle myosin heavy chain A, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain IIa, Non-muscle myosin heavy chain IIb, Non-muscle myosin heavy chain IIc, Nonmuscle myosin II heavy chain B, Nonmuscle myosin heavy chain II A, OTTMUSP00000019210, PHF-tau, PPND, PPP1R103, Paired helical filament-tau, Protein phosphatase 1, regulatory subunit 103, RNPTAU, RP24-311F12.2, SCAN1, STK33_HUMAN, Serine threonine kinase 33, Serine/threonine kinase 33, Serine/threonine-protein kinase 33, TAU_HUMAN, TYDP, TYDP1_HUMAN, Tauopathy and respiratory failure, Tauopathy and respiratory failure, included, Tyr-DNA phosphodiesterase 1, Tyrosyl-DNA phosphodiesterase 1, adult 1, cardiac muscle alpha isoform, non-muscle IIa, non-muscle IIb, non-muscle IIc, pTau, type A, type B

2 Images
Chemical Structure - (±)-Blebbistatin, inhibitor of myosin II (AB120425)
  • Chemical Structure

Lab

Chemical Structure - (±)-Blebbistatin, inhibitor of myosin II (AB120425)

2D chemical structure image of ab120425, Blebbistatin, inhibitor of myosin II

Functional Studies - (±)-Blebbistatin, inhibitor of myosin II (AB120425)
  • FuncS

Unknown

Functional Studies - (±)-Blebbistatin, inhibitor of myosin II (AB120425)

ab32084 staining paxillin in MEF1 cells treated with (+/-)-blebbistatin (ab120425), by ICC/IF. Decreased membrane expression of paxillin correlates with increased concentration of (+/-)-blebbistatin, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120425 ((+/-)-blebbistatin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32084 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Key facts

CAS number

674289-55-5

Purity

>99%

Form

Solid

form

Molecular weight

292.3 Da

Molecular formula

C<sub>1</sub><sub>8</sub>H<sub>1</sub><sub>6</sub>N<sub>2</sub>O<sub>2</sub>

PubChem

3476986

Nature

Synthetic

Solubility

Soluble in DMSO to 100 mM

Biochemical name

Blebbistatin

Biological description

Cell-permeable, reversible inhibitor of non-muscle myosin II. Shows high affinity and selectivity for vertebrate and invertebrate Myosin II (IC50 values 0.5-5 μM). Inhibits actin-activated MgATPase activity and *in vitro* motility of class II myosins. Also inhibits contraction of the cytokinetic ring. Blocks cell blebbing and disrupts directed cell migration and cytokinesis.

Canonical smiles

CC1=CC2=C(C=C1)N=C3C(C2=O)(CCN3C4=CC=CC=C4)O

InChi

InChI=1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3

InChiKey

LZAXPYOBKSJSEX-UHFFFAOYSA-N

IUPAC Name

3a-hydroxy-6-methyl-1-phenyl-2,3-dihydropyrrolo[2,3-b]quinolin-4-one

Product details

Check out our range of myosin inhibitor biochemicals here

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
Handling instructions

Need more advice on solubility, usage and handling? Please visit our our product support page for more details.

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Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dysfunctional myosin proteins can link to a variety of conditions. MYH6 mutations for instance associate with cardiomyopathies which affect heart muscle function and structure. Another significant disorder is cancer where abnormal myosin expression or mutation promotes tumor progression and metastasis. Connections with tau highlight risk factors in neurodegenerative diseases where tau protein aggregation can influence cellular systems. The myosin-related dysfunctions influence diseases by acting in concert with other proteins as seen with TDP1 in neurodegeneration or STK33 in certain cancers reflecting the pervasive impact of myosin functionality in health and disease.
Pathways

Myosin and its variants participate prominently in the actin cytoskeleton signaling pathway. This pathway orchestrates cellular movement and structure important for proliferation and morphogenesis. Myosin's role involves coordinated function with proteins like actin itself tropomyosin and myosin light chains facilitating intracellular transport mechanisms. Additionally myosin II has interactions within the Rho GTPase signaling pathway contributing further to regulation of the actin cytoskeleton and influencing processes like cell migration and focal adhesion dynamics.

Biological function summary

These myosins assume pivotal roles in maintaining cell structure and function. They contribute significantly to cellular adhesion migration and division by interfacing systematically within the cytoskeleton. In contractile cells myosin works as part of a motor protein complex yet in non-muscle cells its responsibilities extend to cell shape maintenance and cytokinesis. These proteins directly influence cellular signaling dynamics and mechanotransduction. The non-muscle myosin IIA for example is critical for maintaining epithelial cell tight junctions emphasizing the structural diversity and importance of myosins across cell types.

Myosin and its variants such as the non-muscle heavy chain myosin tau MYH6 non-muscle myosin IIA and Myh14 serve mechanical roles in cellular processes. These proteins are part of class II myosins commonly referred to as myosin II with each having a different function based on tissue distribution. The molecular mass of these myosins can vary exemplified by MYH6 which weighs approximately 223 kDa. Expression for these proteins varies: MYH6 is found in cardiac muscle while non-muscle myosin IIA and Myh14 are predominantly expressed in non-muscle cells. Myosin interacts with actin filaments to mediate cellular contractility and motility executing force generation through ATP hydrolysis.

Product protocols

Publications (42)

Recent publications for all applications. Explore the full list and refine your search

Journal of the Association for Research in Otolaryngology : JARO 26:225-238 PubMed40240732

2025

Influence of Myosin Regulatory Light Chain and Myosin Light Chain Kinase on the Physiological Function of Inner Ear Hair Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ryohei Oya,Kwang Min Woo,Brian Fabella,R G Alonso,Paloma Bravo,A J Hudspeth

Nature neuroscience 28:49-61 PubMed39623218

2024

Membrane mechanics dictate axonal pearls-on-a-string morphology and function.

Applications

Unspecified application

Species

Unspecified reactive species

Jacqueline M Griswold,Mayte Bonilla-Quintana,Renee Pepper,Christopher T Lee,Sumana Raychaudhuri,Siyi Ma,Quan Gan,Sarah Syed,Cuncheng Zhu,Miriam Bell,Mitsuo Suga,Yuuki Yamaguchi,Ronan Chéreau,U Valentin Nägerl,Graham Knott,Padmini Rangamani,Shigeki Watanabe

Theranostics 14:6625-6651 PubMed39479451

2024

Brain-to-heart cholinergic synapse-calcium signaling mediates ischemic stroke-induced atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Yingran Liang,Gongxin Wang,Siwen Fan,Junyi Zhang,Shuang He,Guixiang Pan,Guoliang Hao,Yan Zhu

Nature communications 15:7996 PubMed39266508

2024

Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions.

Applications

Unspecified application

Species

Unspecified reactive species

Pengcheng Yang,Lihang Zhu,Shiya Wang,Jixing Gong,Jonathan Nimal Selvaraj,Lincai Ye,Hanxiao Chen,Yaoyao Zhang,Gongxin Wang,Wanjun Song,Zilong Li,Lin Cai,Hao Zhang,Donghui Zhang

The Journal of cell biology 223: PubMed38713825

2024

Actomyosin-II protects axons from degeneration induced by mild mechanical stress.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaorong Pan,Yiqing Hu,Gaowei Lei,Yaxuan Wei,Jie Li,Tongshu Luan,Yunfan Zhang,Yuanyuan Chu,Yu Feng,Wenrong Zhan,Chunxia Zhao,Frédéric A Meunier,Yifan Liu,Yi Li,Tong Wang

Cell stem cell 31:640-656.e8 PubMed38701758

2024

Lumen expansion is initially driven by apical actin polymerization followed by osmotic pressure in a human epiblast model.

Applications

Unspecified application

Species

Unspecified reactive species

Dhiraj Indana,Andrei Zakharov,Youngbin Lim,Alexander R Dunn,Nidhi Bhutani,Vivek B Shenoy,Ovijit Chaudhuri

American journal of physiology. Heart and circulatory physiology 326:H203-H215 PubMed37975708

2023

A comprehensive protocol combining in vivo and ex vivo electrophysiological experiments in an arrhythmogenic animal model.

Applications

Unspecified application

Species

Unspecified reactive species

Mathias Nyman,Tomas O Stølen,Anne Berit Johnsen,Karin Garten,Francis L Burton,Godfrey L Smith,Jan Pål Loennechen

Nature communications 14:6226 PubMed37803005

2023

Chronological adhesive cardiac patch for synchronous mechanophysiological monitoring and electrocoupling therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Chaojie Yu,Mingyue Shi,Shaoshuai He,Mengmeng Yao,Hong Sun,Zhiwei Yue,Yuwei Qiu,Baijun Liu,Lei Liang,Zhongming Zhao,Fanglian Yao,Hong Zhang,Junjie Li

Proceedings of the National Academy of Sciences of the United States of America 120:e2308941120 PubMed37782785

2023

A 3D biomimetic model of lymphatics reveals cell-cell junction tightening and lymphedema via a cytokine-induced ROCK2/JAM-A complex.

Applications

Unspecified application

Species

Unspecified reactive species

Esak Lee,Siu-Lung Chan,Yang Lee,William J Polacheck,Sukyoung Kwak,Aiyun Wen,Duc-Huy T Nguyen,Matthew L Kutys,Stella Alimperti,Anna M Kolarzyk,Tae Joon Kwak,Jeroen Eyckmans,Diane R Bielenberg,Hong Chen,Christopher S Chen

Nature communications 14:5166 PubMed37620390

2023

Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Jiang,Hong-Yu Luo,Xinpeng Xu,Shuo-Xing Dou,Wei Li,Dongshi Guan,Fangfu Ye,Xiaosong Chen,Ming Guo,Peng-Ye Wang,Hui Li
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