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AB146379

Bluo-Gal, Chromogenic beta-galactosidase substrate

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(1 Publication)

MW 374.2 g/mol, Purity >98%. Chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Designed as an alternative to X-Gal (ab144388) in blue-white selection of recombinant bacterial colonies with the lac+ phenotype, producing a darker blue color.
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Chemical Structure - Bluo-Gal, Chromogenic beta-galactosidase substrate (AB146379)
  • Chemical Structure

Lab

Chemical Structure - Bluo-Gal, Chromogenic beta-galactosidase substrate (AB146379)

2D chemical structure image of ab146379, Bluo-Gal, Chromogenic beta-galactosidase substrate

Key facts

CAS number

97753-82-7

Purity

>98%

Molecular weight

374.2 g/mol

Molecular formula

C<sub>1</sub><sub>4</sub>H<sub>1</sub><sub>6</sub>BrNO<sub>6</sub>

Nature

Synthetic

Solubility

Soluble in DMSO to 100 mM

Soluble in DMF to 100 mM

Biological description

Chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Designed as an alternative to X-Gal (ab144388) in blue-white selection of recombinant bacterial colonies with the lac+ phenotype, producing a darker blue color.

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Product details

Instructions for use: Add 5-Bromo-3-indoxyl- beta-D-galacto-pyranoside (BB1182) as concentrated stock solution (solvent DMSO or DMF) to autoclaved media

Recommended working concentration 0.1-0.3 mM (1-3 mL of 100 mM stock solution per L)

For best results also add 0.1-1 mM of the inducer isopropyl-beta-D-thiogalactopyrano-side (IPTG) to growth media

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store in the dark|Store under desiccating conditions|This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes
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Product protocols

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:27311 PubMed39516286

2024

Immunogenicity of intraperitoneal and intranasal liposome adjuvanted VLP vaccines against SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Monrat Chulanetra,Primana Punnakitikashem,Kodchakorn Mahasongkram,Wanpen Chaicumpa,Kantaphon Glab-Ampai
View all publications
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