MW 307.1 Da, Purity >98%. Thymidine analog, naturally incorporates into proliferating cells. Labels DNA, useful marker for study of DNA synthesis. Decreases TTP pools in vitro. Antitumor and radiation-sensitizing agent. Active in vitro and in vivo.
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- NPJ Parkinson's disease 10:122024A potential patient stratification biomarker for Parkinson´s disease based on LRRK2 kinase-mediated centrosomal alterations in peripheral blood-derived cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYahaira Naaldijk,Belén Fernández,Rachel Fasiczka,Elena Fdez,Coline Leghay,Ioana Croitoru,John B Kwok,Yanisse Boulesnane,Amelie Vizeneux,Eugenie Mutez,Camille Calvez,Alain Destée,Jean-Marc Taymans,Ana Vinagre Aragon,Alberto Bergareche Yarza,Shalini Padmanabhan,Mario Delgado,Roy N Alcalay,Zac Chatterton,Nicolas Dzamko,Glenda Halliday,Javier Ruiz-Martínez,Marie-Christine Chartier-Harlin,Sabine HilfikerPubMed 38191886
- PLoS biology 21:e30024352023Distinct stem-like cell populations facilitate functional regeneration of the Cladonema medusa tentacle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSosuke Fujita,Mako Takahashi,Gaku Kumano,Erina Kuranaga,Masayuki Miura,Yu-Ichiro NakajimaPubMed 38127832
- Nature communications 14:84842023Adult stem cell activity in naked mole rats for long-term tissue maintenance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShamir Montazid,Sheila Bandyopadhyay,Daniel W Hart,Nan Gao,Brian Johnson,Sri G Thrumurthy,Dustin J Penn,Bettina Wernisch,Mukesh Bansal,Philipp M Altrock,Fabian Rost,Patrycja Gazinska,Piotr Ziolkowski,Bu'Hussain Hayee,Yue Liu,Jiangmeng Han,Annamaria Tessitore,Jana Koth,Walter F Bodmer,James E East,Nigel C Bennett,Ian Tomlinson,Shazia IrshadPubMed 38123565
- Scientific reports 13:226132023Impaired neuronal activity as a potential factor contributing to the underdeveloped cerebrovasculature in a young Parkinson's disease mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Young Jeong,Hyun Jung Lee,Namsuk Kim,Yan Li,Jong-Cheol Rah,Won-Jong OhPubMed 38114623
- Nature communications 14:83722023BRD9 determines the cell fate of hematopoietic stem cells by regulating chromatin state.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuran Xiao,Shinji Kondo,Masaki Nomura,Shinichiro Kato,Koutarou Nishimura,Weijia Zang,Yifan Zhang,Tomohiro Akashi,Aaron Viny,Tsukasa Shigehiro,Tomokatsu Ikawa,Hiromi Yamazaki,Miki Fukumoto,Atsushi Tanaka,Yasutaka Hayashi,Yui Koike,Yumi Aoyama,Hiromi Ito,Hiroyoshi Nishikawa,Toshio Kitamura,Akinori Kanai,Akihiko Yokoyama,Tohru Fujiwara,Susumu Goyama,Hideki Noguchi,Stanley C Lee,Atsushi Toyoda,Kunihiko Hinohara,Omar Abdel-Wahab,Daichi InouePubMed 38102116
- Cell 186:1930-1949.e312023Pyramidal neurons form active, transient, multilayered circuits perturbed by autism-associated mutations at the inception of neocortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin Munz,Arjun Bharioke,Georg Kosche,Verónica Moreno-Juan,Alexandra Brignall,Tiago M Rodrigues,Alexandra Graff-Meyer,Talia Ulmer,Stephanie Haeuselmann,Dinko Pavlinic,Nicole Ledergerber,Brigitte Gross-Scherf,Balázs Rózsa,Jacek Krol,Simone Picelli,Cameron S Cowan,Botond RoskaPubMed 37071993
- PloS one 18:e02821742023Rhodopsin-positive cell production by intravitreal injection of small molecule compounds in mouse models of retinal degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuya Fujii,Mitsuru Arima,Yusuke Murakami,Koh-Hei SonodaPubMed 36821627
- Proceedings of the National Academy of Sciences of the United States of America 120:e22159061202023Self-renewing macrophages in dorsal root ganglia contribute to promote nerve regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Feng,Vishnu Muraleedharan Saraswathy,Mayssa H Mokalled,Valeria CavalliPubMed 36763532
- International journal of molecular sciences 24:2023Adipose Tissue-Derived Extracellular Vesicles Contribute to Phenotypic Plasticity of Prostate Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAllison Mathiesen,Bronson Haynes,Ryan Huyck,Michael Brown,Anca DobrianPubMed 36674745
- Cell reports 41:1118802022Secreted phosphoprotein 1 slows neurodegeneration and rescues visual function in mouse models of aging and glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Li,Tatjana C JakobsPubMed 36577373
Key facts
- CAS number
- 59-14-3
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 307.1 Da
- Molecular formula
- C9H11BrN2O5
- PubChem identifier
- 6035
- Nature
- Synthetic
Associated Products
Select an associated product type
2 products for Alternative Product
Alternative names
KITH_HUMAN, TK 1, TK 2, Thymidine kinase, Thymidine kinase 1, Thymidine kinase 1 soluble, Thymidine kinase 1 soluble isoform, Thymidine kinase cytosolic, Tk1a, Tk1b, cytosolic
Recommended products
MW 307.1 Da, Purity >98%. Thymidine analog, naturally incorporates into proliferating cells. Labels DNA, useful marker for study of DNA synthesis. Decreases TTP pools in vitro. Antitumor and radiation-sensitizing agent. Active in vitro and in vivo.
Key facts
- CAS number
- 59-14-3
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 307.1 Da
- Molecular formula
- C9H11BrN2O5
- PubChem identifier
- 6035
- Nature
- Synthetic
- Solubility
Dimethylacetamide ~ 200 (mg/mL).
Soluble in water to 50 mM.
- Biochemical name
- 5-Bromo-2'-deoxyuridine
- Biological description
Thymidine analog, naturally incorporates into proliferating cells. Labels DNA, useful marker for study of DNA synthesis. Decreases TTP pools in vitro. Antitumor and radiation-sensitizing agent. Active in vitro and in vivo.
- Canonical SMILES
- C1C(C(OC1N2C=C(C(=O)NC2=O)Br)CO)O
- Isomeric SMILES
- C1[C@@H]([C@H](O[C@H]1N2C=C(C(=O)NC2=O)Br)CO)O
- InChI
- InChI=1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
- InChIKey
- WOVKYSAHUYNSMH-RRKCRQDMSA-N
- IUPAC name
- 5-bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunocytochemistry/ Immunofluorescence - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
Alexa Fluor® 647 Anti-BrdU antibody [BU1/75 (ICR1)] ab220075 staining BrdU in HeLa cells. Untreated and BrdU treated (ab142567, 10uM for 24 hours) cells were fixed with 100% methanol (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 647 Anti-BrdU antibody [BU1/75 (ICR1)] ab220075 at 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).
Chemical Structure - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
2D chemical structure image of ab142567, BrdU (5-bromo-2'-deoxyuridine), Thymidine analog
Massey, AJ PLoS One. 2015 Jul 28;10(7):e0134306. doi: 10.1371/journal.pone.0134306. eCollection 2015. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
Estimation of S-phase Duration using EdU-BrdU Dual Pulse Labelling.(From Figure 5A of Massey)
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