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MW 994.9 Da, Purity >96%. Cell permeant probe used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent calcein after acetoxymethyl ester hydrolysis by intracellular esterases.


Images

Chemical Structure - Calcein AM, fluorescent dye for cell viability (AB141420), expandable thumbnail

Publications

Key facts

CAS number

148504-34-1

Purity

> 96%

Form

Solid

Molecular weight

994.9 Da

Molecular formula

C46H46N2O23

PubChem identifier

390986

Nature

Synthetic

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MW 994.9 Da, Purity >96%. Cell permeant probe used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent calcein after acetoxymethyl ester hydrolysis by intracellular esterases.

Key facts

Purity

> 96%

PubChem identifier

390986

Solubility

Soluble in DMSO.

Biochemical name

Calcein AM

Biological description

Cell permeant probe used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent calcein after acetoxymethyl ester hydrolysis by intracellular esterases.

Canonical SMILES

CC(=O)OCOC(=O)CN(CC1=CC2=C(C=C1OC(=O)C)OC3=C(C24C5=CC=CC=C5C(=O)O4)C=C(C(=C3)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

InChI

InChI=1S/C46H46N2O23/c1-25(49)60-21-64-41(55)17-47(18-42(56)65-22-61-26(2)50)15-31-11-35-39(13-37(31)68-29(5)53)70-40-14-38(69-30(6)54)32(12-36(40)46(35)34-10-8-7-9-33(34)45(59)71-46)16-48(19-43(57)66-23-62-27(3)51)20-44(58)67-24-63-28(4)52/h7-14H,15-24H2,1-6H3

InChIKey

BQRGNLJZBFXNCZ-UHFFFAOYSA-N

IUPAC name

acetyloxymethyl 2-[[2-(acetyloxymethoxy)-2-oxoethyl]-[[3',6'-diacetyloxy-7'-[[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]methyl]-3-oxospiro[2-benzofuran-1,9'-xanthene]-2'-yl]methyl]amino]acetate

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

It is important to note that this product is reported to be light sensitive, Store in the dark, Store under desiccating conditions

Product promise

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Terms & Conditions.

1 product image

  • Chemical Structure - Calcein AM, fluorescent dye for cell viability (ab141420), expandable thumbnail

    Chemical Structure - Calcein AM, fluorescent dye for cell viability (ab141420)

    2D chemical structure image of ab141420, Calcein AM, fluorescent dye for cell viability

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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