Calcein Blue AM acetate

Key facts

CAS number

168482-84-6

Purity

>95%

Form

Solid

form

Molecular weight

465.4 Da

Molecular formula

C₂₁H₂₃NO₁₁

PubChem

71774480

Nature

Synthetic

Solubility

Soluble in DMSO.

Biochemical name

Calcein blue, AM

Biological description

Blue fluorescent cell viability probe which won't overlap with green fluorescence from other probes. Penetrates live cell membranes and is virtually non-fluorescent. Upon ubiquitous esterase enzyme activity it produces a bright blue fluorescent that is trapped in live cells - convenient for measurement by epifluorescence light microscopy or flow cytometry. Useful to measure the total number of live versus dead cells - dye leaks out quickly due to the compromised membrane in dead cells resulting in no staining.

Canonical smiles

CC1=CC(=O)OC2=C1C=CC(=C2CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)O

InChi

InChI=1S/C21H23NO11/c1-12-6-18(26)33-21-15(12)4-5-17(25)16(21)7-22(8-19(27)31-10-29-13(2)23)9-20(28)32-11-30-14(3)24/h4-6,25H,7-11H2,1-3H3

InChiKey

GSFJGCPBBHLSPH-UHFFFAOYSA-N

IUPAC Name

acetyloxymethyl 2-[[2-(acetyloxymethoxy)-2-oxoethyl]-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetate

Excitation/Emission

Ex: 360nm, Em: 450nm