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AB145415

Carboxyl-DCFDA (5(6)-Carboxy-2',7'-dichlorofluorescein diacetate), Fluorogenic esterase substrate

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MW 529.28 Da, Purity >85%. Amine-reactive, fluorogenic, non-selective esterase substrate. Hydrolyses to DFCA (ab145439) fluorophore intracellularly. Labels intact cells. 2,7-Dichlorofluorescein (ab146332) derivative and DCFDA N-succinimidyl ester (ab145286) precursor.
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Chemical Structure - Carboxyl-DCFDA (5(6)-Carboxy-2',7'-dichlorofluorescein diacetate), Fluorogenic esterase substrate (AB145415)
  • Chemical Structure

Lab

Chemical Structure - Carboxyl-DCFDA (5(6)-Carboxy-2',7'-dichlorofluorescein diacetate), Fluorogenic esterase substrate (AB145415)

2D chemical structure image of ab145415, DFCA DA (5(6)-Carboxy-2',7'-dichlorofluorescein diacetate), Fluorogenic esterase substrate

Key facts

CAS number

127770-45-0

Purity

>85%

Form

Solid

form

Molecular weight

529.28 Da

Molecular formula

C<sub>2</sub><sub>5</sub>H<sub>1</sub><sub>4</sub>Cl<sub>2</sub>O<sub>9</sub>

Nature

Synthetic

Solubility

Soluble in DMSO, DMF or methanol.

Biological description

Amine-reactive, fluorogenic, non-selective esterase substrate. Hydrolyses to DFCA (ab145439) fluorophore intracellularly. Labels intact cells. 2,7-Dichlorofluorescein (ab146332) derivative and DCFDA N-succinimidyl ester (ab145286) precursor.

Canonical smiles

CC(O)=O.CC(=O)OC1=CC2=C(C=C1Cl)C1(OC(=O)C3=C1C=CC=C3)C1=CC(Cl)=C(OC(C)=O)C=C1O2

Excitation/Emission

Ex: 529nm, Em: 470nm

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Complementary Products

Biochemicals

AB145286

Carboxy-DCFDA N-succinimidyl ester, Fluorogenic esterase substrate

Chemical Structure - Carboxy-DCFDA N-succinimidyl ester, Fluorogenic esterase substrate (AB145286)

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Assay Kits

AB113851

DCFDA / H2DCFDA - Cellular ROS Assay Kit

Cellular Activity - DCFDA / H2DCFDA - Cellular ROS Assay Kit (AB113851)

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Assay Kits

AB186027

Cellular ROS Assay Kit (Red)

Functional Studies - Cellular ROS Assay Kit (Red) (AB186027)

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Assay Kits

AB139473

Cellular ROS/RNS Assay Kit

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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