MW 344.84 g/mol, Purity >99%. Non-selective P450 cytochrome inhibitor (Ki = 0.02-67 μM for P450 cyctochrome subtypes). Potent inhibitor of 34A (Ki = 20 nM). Antifungal agent.
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Key facts
- CAS number
- 23593-75-1
- Purity
- > 99%
- Form
- Solid
- Molecular weight
- 344.84 g/mol
- Molecular formula
- C22H17ClN2
- Nature
- Synthetic
Recommended products
MW 344.84 g/mol, Purity >99%. Non-selective P450 cytochrome inhibitor (Ki = 0.02-67 μM for P450 cyctochrome subtypes). Potent inhibitor of 34A (Ki = 20 nM). Antifungal agent.
Key facts
- CAS number
- 23593-75-1
- Purity
- > 99%
- Form
- Solid
- Molecular weight
- 344.84 g/mol
- Molecular formula
- C22H17ClN2
- Nature
- Synthetic
- Solubility
Soluble in DMSO to 75 mM.
Soluble in ethanol to 100 mM.
- Biological description
Non-selective P450 cytochrome inhibitor (Ki = 0.02-67 μM for P450 cyctochrome subtypes). Potent inhibitor of 34A (Ki = 20 nM). Antifungal agent.
- IUPAC name
- 1-(2-Chloro-α,α-diphenylbenzyl)imidazole
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
- Storage information
- The product can be stored for up to 12 months
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2 product images
Chemical Structure - Clotrimazole, Non-selective P450 cytochrome inhibitor (ab120744)
2D chemical structure image of ab120744, Clotrimazole, Non-selective P450 cytochrome inhibitor
Functional Studies - Clotrimazole, Non-selective P450 cytochrome inhibitor (ab120744)
HepG2 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of clotrimazole (ab120744)in DMSO. Increased expression of MRP3 (Anti-MRP3 antibody [M3II-9] ab3375) correlates with an increase in clotrimazole concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with Anti-MRP3 antibody [M3II-9] ab3375 at 1/50 dilution and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/10000 dilution and visualised using ECL development solution.
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