Cryptotanshinone, STAT3 inhibitor

• FuncS

Unknown

Functional Studies - Cryptotanshinone, STAT3 inhibitor (AB120666)

MCF7 cells were incubated at 37°C for 4h with vehicle control (0 μM) and different concentrations of cryptotanshinone (ab120666). Increased expression of JNK1+JNK2 (phospho T183 + Y185) in MCF7cells correlates with an increase in cryptotanshinone concentration as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate) 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab4821 at at 1/1000 dilution and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

• Chemical Structure

Lab

Chemical Structure - Cryptotanshinone, STAT3 inhibitor (AB120666)

2D chemical structure image of ab120666, Cryptotanshinone, STAT3 inhibitor