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AB222964

CUR 61414, Hedgehog signaling pathway antagonist

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MW 550.7 Da, Purity >98%. Novel, cell permeable Hedgehog signaling pathway antagonist (IC50 = 100-200 nM). Selectively binds to smoothened (Smo) (Ki = 44 nM). Blocks the formation of and induces the regression of basaloid lesions in skin punches and shrinks UV-induced basaloid lesions in adult mouse skin. Inhibits proliferation and induces apoptosis in tumor cells without affecting non-tumor cells.

View Alternative Names

FZD11, Frizzled family member 11, GX, Gx protein, HHG-1, HLP 3, HPE 3, Holoprosencephaly 3, MCOPCB5, Protein Gx, SHH_HUMAN, SMMC I, SMOH, SMO_HUMAN, Smoothened frizzled family receptor, Smoothened homolog, Smoothened homolog (Drosophila), Smoothened homolog precursor (SMO) (Gx protein), Smoothened seven transmembrane spanning receptor, Sonic Hedgehog (Drosophila) homolog, Sonic hedgehog homolog, Sonic hedgehog protein, Sonic hedgehog protein C-product, TPT, TPTPS, sonic hedgehog homolog (Drosophila)

Key facts

CAS number

334998-36-6

Purity

>98%

Form

Solid

form

Molecular weight

550.7 Da

Molecular formula

C<sub>3</sub><sub>1</sub>H<sub>4</sub><sub>2</sub>N<sub>4</sub>O<sub>5</sub>

PubChem

10392802

Nature

Synthetic

Solubility

Soluble in DMSO to 70 mM

Soluble in ethanol to 30 mM

Biochemical name

N-((3S,5S)-1-(Benzo[d][1,3]dioxol-5-ylmethyl)-5-(piperazine-1-carbonyl)pyrrolidin-3-yl)-N-(3-methoxybenzyl)-3,3-dimethylbutanamide

Biological description

Novel, cell permeable Hedgehog signaling pathway antagonist (IC50 = 100-200 nM). Selectively binds to smoothened (Smo) (Ki = 44 nM). Blocks the formation of and induces the regression of basaloid lesions in skin punches and shrinks UV-induced basaloid lesions in adult mouse skin. Inhibits proliferation and induces apoptosis in tumor cells without affecting non-tumor cells.

Canonical smiles

CC(C)(C)CC(=O)N(CC1=CC(=CC=C1)OC)C2CC(N(C2)CC3=CC4=C(C=C3)OCO4)C(=O)N5CCNCC5

Isomeric smiles

CC(C)(C)CC(=O)N(CC1=CC(=CC=C1)OC)[C@H]2C[C@H](N(C2)CC3=CC4=C(C=C3)OCO4)C(=O)N5CCNCC5

InChi

InChI=1S/C31H42N4O5/c1-31(2,3)17-29(36)35(19-22-6-5-7-25(14-22)38-4)24-16-26(30(37)33-12-10-32-11-13-33)34(20-24)18-23-8-9-27-28(15-23)40-21-39-27/h5-9,14-15,24,26,32H,10-13,16-21H2,1-4H3/t24-,26-/m0/s1

InChiKey

WPHXYKUPFJRJDK-AHWVRZQESA-N

IUPAC Name

N-[(3S,5S)-1-(1,3-benzodioxol-5-ylmethyl)-5-(piperazine-1-carbonyl)pyrrolidin-3-yl]-N-[(3-methoxyphenyl)methyl]-3,3-dimethylbutanamide

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Smoothened (SMO) protein also known as Sonic Hedgehog receptor plays a mechanical role in the Hedgehog (Hh) signaling pathway. This protein acts as a transmembrane receptor that transmits signals from the cell surface to the interior. Smoothened has a molecular mass of approximately 86 kDa and is expressed in several tissues including the brain kidney and liver. Its activity requires the presence of another protein Patched which regulates Smoothened by inhibiting or permitting its signaling depending on Hedgehog ligand binding.
Biological function summary

Smoothened is essential for the Hh signaling pathway which is pivotal for embryonic development and tissue regeneration. In this context Smoothened participates in a complex that includes Patched and Gli family transcription factors. This complex orchestrates the transcription of target genes necessary for cellular proliferation differentiation and tissue organization. Smoothened’s role in activating transcription factors makes it integral to modulating gene expression in response to external Hh signals.

Pathways

Smoothened is a central component of the Hedgehog signaling pathway which plays an important role in cell differentiation and organogenesis. Two of the significant pathways it influences are the Sonic Hedgehog (Shh) and Indian Hedgehog (Ihh) signaling pathways. Within these pathways Smoothened interacts closely with the protein Patched which inhibits Smoothened in the absence of Hedgehog ligands. The downstream activation of Smoothened results in the modulation of Gli transcription factors altering the expression of various developmental genes.

Smoothened is closely linked to conditions such as basal cell carcinoma and medulloblastoma. Dysregulation or mutations in Smoothened can lead to aberrant activation of Hedgehog signaling contributing to the development and progression of these cancers. The protein Gli as part of the same signaling cascade is also heavily involved in the transcriptional changes associated with these disorders. Inhibitors like Cyclopamine sometimes in an ethanol solution target Smoothened to mitigate its pathological signaling and offer therapeutic approaches for these types of cancers.
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Product protocols

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Complementary Products

Biochemicals

AB120933

Purmorphamine, Smoothened receptor agonist

Chemical Structure - Purmorphamine, Smoothened receptor agonist (AB120933)

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Biochemicals

AB120392

Cyclopamine, Hh signaling inhibitor

Chemical Structure - Cyclopamine, Hh signaling inhibitor (AB120392)

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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