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AB146631

DAF-2 DA (DMSO solution), fluorescent nitric oxide probe

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(1 Publication)

MW 446.4 Da, Purity >98%. Fluorescent nitric oxide probe; membrane-permeable. Once inside cell it reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein DAF-2T (ab145473). Determines nitric oxide synthase activity. Has a 2-5 nM detection limit at neutral pH.

Also available as a solid (ab145283).
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Chemical Structure - DAF-2 DA (DMSO solution), fluorescent nitric oxide probe (AB146631)
  • Chemical Structure

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Chemical Structure - DAF-2 DA (DMSO solution), fluorescent nitric oxide probe (AB146631)

2D chemical structure image of ab146631, DAF-2 DA (DMSO solution), fluorescent nitric oxide probe

Key facts

CAS number

205391-02-2

Purity

>98%

Form

Liquid

form

Molecular weight

446.4 Da

Molecular formula

C<sub>2</sub><sub>4</sub>H<sub>1</sub><sub>8</sub>N<sub>2</sub>O<sub>7</sub>

PubChem

6603693

Nature

Synthetic

Biochemical name

4,5-Diaminofluorescein diacetate

Biological description

Fluorescent nitric oxide probe; membrane-permeable. Once inside cell it reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein DAF-2T (ab145473). Determines nitric oxide synthase activity. Has a 2-5 nM detection limit at neutral pH.

Also available as a solid (ab145283).

Canonical smiles

CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=CC(=C(C=C5C(=O)O3)N)N

InChi

InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3

InChiKey

PTSUYDXEEKDBQU-UHFFFAOYSA-N

IUPAC Name

(6'-acetyloxy-5,6-diamino-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate

Excitation/Emission

Ex: 485nm, Em: 538nm

Product details

This product is manufactured by BioVision, an Abcam company and was previously called 2753 DAF-2 diacetate, EZSolution™. 2753-100 is the same size as the 100 μg size of ab146631.

Supplied in DMSO (5 mM).

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store in the dark|Store under desiccating conditions|This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes

Product protocols

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 7: PubMed35700057

2022

EPCR-PAR1 biased signaling regulates perfusion recovery and neovascularization in peripheral ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena L Bochenek,Rajinikanth Gogiraju,Stefanie Großmann,Janina Krug,Jennifer Orth,Sabine Reyda,George S Georgiadis,Henri M Spronk,Stavros Konstantinides,Thomas Münzel,John H Griffin,Philipp Wild,Christine Espinola-Klein,Wolfram Ruf,Katrin Schäfer
View all publications

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