JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB145295

DAF-FM DA, Nitric oxide quantification reagent

Be the first to review this product! Submit a review

|

(1 Publication)

MW 496.42 g/mol, Purity >98%. Cell permeable reagent for quantification of low concentrations of nitric oxide in solution. Essentially nonfluorescent until it is hydrolyzed to DAF-FM (ab145297) by intracellular esterases and reacts with NO to form a fluorescent benzotriazole.
1 Images
Chemical Structure - DAF-FM DA, Nitric oxide quantification reagent (AB145295)
  • Chemical Structure

Lab

Chemical Structure - DAF-FM DA, Nitric oxide quantification reagent (AB145295)

2D chemical structure image of ab145295, DAF-FM DA, Nitric oxide quantification reagent

Key facts

CAS number

254109-22-3

Purity

>98%

Molecular weight

496.42 g/mol

Molecular formula

C<sub>2</sub><sub>5</sub>H<sub>1</sub><sub>8</sub>F<sub>2</sub>N<sub>2</sub>O<sub>7</sub>

Nature

Synthetic

Solubility

Soluble in DMSO

Biological description

Cell permeable reagent for quantification of low concentrations of nitric oxide in solution. Essentially nonfluorescent until it is hydrolyzed to DAF-FM (ab145297) by intracellular esterases and reacts with NO to form a fluorescent benzotriazole.

InChi

InChI=1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

InChiKey

BEVHTVRRVVEMEF-UHFFFAOYSA-N

IUPAC Name

3-Amino-4-(N-methylamino)-2',7'-difluorofluorescein diacetate

Excitation/Emission

Ex: 500nm, Em: 515nm

style
left-column

Complementary Products

Biochemicals

AB145283

DAF-2 DA, Fluorescent nitric oxide probe

Chemical Structure - DAF-2 DA, Fluorescent nitric oxide probe (AB145283)

  • 0
  • 0
Biochemicals

AB146631

DAF-2 DA (DMSO solution), fluorescent nitric oxide probe

Chemical Structure - DAF-2 DA (DMSO solution), fluorescent nitric oxide probe (AB146631)

  • 0
  • 0
Sample Prep & Detection Kits

AB65400

Plasma Membrane Protein Extraction Kit

Western blot - Plasma Membrane Protein Extraction Kit (AB65400)

  • 0
  • 0
Primary Antibodies

AB180958

Anti-S tag antibody [EPR12996]

RabMAb|Recombinant|Lab Essentials

Western blot - Anti-S tag antibody [EPR12996] (AB180958)

  • 0
  • 0
style
left-column
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
style
left-column
style
left-column

Product protocols

style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadk3126 PubMed38489361

2024

-nitrosylation of a receptor-like cytoplasmic kinase regulates plant immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Beimi Cui,Qiaona Pan,Wenqiang Cui,Yiqin Wang,Verity I P Loake,Shuguang Yuan,Fengquan Liu,Gary J Loake
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com