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AB145297

DAF-FM, Fluorescent probe for nitric oxide quantification

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MW 412.34 g/mol, Purity >98%. Highly sensitive, photo-stable fluorescent probe for quantification of low concentrations of nitric oxide (NO). More sensitive to nitric oxide (NO) than DAF-2 (ab145269). More photo-stable and less pH sensitive than DAF-2. Not as cell-permeable as the diacetate derivative, DAF-FM DA (ab145295), but it does not need to be activated by cytosolic enzymes. Essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole.
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Chemical Structure - DAF-FM, Fluorescent probe for nitric oxide quantification (AB145297)
  • Chemical Structure

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Chemical Structure - DAF-FM, Fluorescent probe for nitric oxide quantification (AB145297)

2D chemical structure image of ab145297, DAF-FM, Fluorescent probe for nitric oxide quantification

Key facts

CAS number

254109-20-1

Purity

>98%

Form

Solid

form

Molecular weight

412.34 g/mol

Molecular formula

C<sub>2</sub><sub>1</sub>H<sub>1</sub><sub>4</sub>F<sub>2</sub>N<sub>2</sub>O<sub>5</sub>

Nature

Synthetic

Solubility

Soluble in DMSO

Biological description

Highly sensitive, photo-stable fluorescent probe for quantification of low concentrations of nitric oxide (NO). More sensitive to nitric oxide (NO) than DAF-2 (ab145269). More photo-stable and less pH sensitive than DAF-2. Not as cell-permeable as the diacetate derivative, DAF-FM DA (ab145295), but it does not need to be activated by cytosolic enzymes. Essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole.

InChi

InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29)

InChiKey

BJTLSPOVXMBXRZ-UHFFFAOYSA-N

IUPAC Name

3-Amino-4-(N-methylamino)-2',7'-difluorofluorescein

Excitation/Emission

Ex: 495nm, Em: 515nm

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Alternative Products

Biochemicals

AB145297

DAF-FM, Fluorescent probe for nitric oxide quantification

Chemical Structure - DAF-FM, Fluorescent probe for nitric oxide quantification (AB145297)

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Complementary Products

Biochemicals

AB145295

DAF-FM DA, Nitric oxide quantification reagent

Chemical Structure - DAF-FM DA, Nitric oxide quantification reagent (AB145295)

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Biochemicals

AB145283

DAF-2 DA, Fluorescent nitric oxide probe

Chemical Structure - DAF-2 DA, Fluorescent nitric oxide probe (AB145283)

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Biochemicals

AB145269

DAF-2, Fluorescent nitric oxide probe

Chemical Structure - DAF-2, Fluorescent nitric oxide probe (AB145269)

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Biochemicals

AB146631

DAF-2 DA (DMSO solution), fluorescent nitric oxide probe

Chemical Structure - DAF-2 DA (DMSO solution), fluorescent nitric oxide probe (AB146631)

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Product promise

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