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AB145248

DAN, Fluorescence determination of NO2-

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MW 158.2 g/mol, Purity >98%. Routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity. Also used as a derivatisation-reagent for the determination of selenium at low detection limits.
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Chemical Structure - DAN, Fluorescence determination of NO2- (AB145248)
  • Chemical Structure

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Chemical Structure - DAN, Fluorescence determination of NO2- (AB145248)

2D chemical structure image of ab145248, DAN, Fluorescence determination of NO2-

Key facts

CAS number

771-97-1

Purity

>98%

Form

Solid

form

Molecular weight

158.2 g/mol

Nature

Synthetic

Solubility

Soluble in DMSO

Biological description

Routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity. Also used as a derivatisation-reagent for the determination of selenium at low detection limits.

InChi

InChI=1S/C10H10N2/c11-9-5-7-3-1-2-4-8(7)6-10(9)12/h1-6H,11-12H2

InChiKey

XTBLDMQMUSHDEN-UHFFFAOYSA-N

IUPAC Name

2,3-Diaminonaphthalene

Excitation/Emission

Ex: 364nm, Em: 406nm

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
The product can be stored for up to 12 months

Product protocols

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