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AB285390

DAPI

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(58 Publications)

DAPI (CAS: 28718-90-3)(ab285390) is a fluorescent, cell-permeable DNA-binding dye and preferentially binds to DNA rich in adenine and thymine. Suitable to use for fluorescence microscopy. DAPI's absorption maximum when bound to double-stranded DNA is at 358 nm and its emission maximum is at 461 nm. MW 350.2, Purity >98%.

- Proven performance: cited in over 55 publications
- Convenient for multiplexing assays as little overlap between green- and red- fluorescence molecules
- Available in different sizes to fit your experimental needs

Key facts

CAS number

28718-90-3

Purity

>98%

Form

Solid

form

Molecular weight

350.2 Da

Molecular formula

C<sub>1</sub><sub>6</sub>H<sub>1</sub><sub>7</sub>Cl<sub>2</sub>N<sub>5</sub>

PubChem

160166

Nature

Synthetic

Solubility

DMSO (>35 mg/ml)

Biochemical name

2-(4-Carbamimidoylphenyl)-1h-indole-6-carboximidamide dihydrochloride

Biological description

A fluorescent, cell-permeable DNA-binding dye. Preferentially binds to DNA rich in adenine and thymine.

Canonical smiles

C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl

InChi

InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChiKey

FPNZBYLXNYPRLR-UHFFFAOYSA-N

IUPAC Name

2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide;dihydrochloride

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB228549

DAPI Staining Solution

Spectrum - DAPI Staining Solution (AB228549)

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  • 1
Reagents, Controls & Accessories

AB104139

Mounting Medium With DAPI - Aqueous, Fluoroshield

Immunohistochemistry (Frozen sections) - Mounting Medium With DAPI - Aqueous, Fluoroshield (AB104139)

  • 5
  • 9
Reagents, Controls & Accessories

AB104135

Anti-Fade Fluorescence Mounting Medium - Aqueous, Fluoroshield

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Fade Fluorescence Mounting Medium - Aqueous, Fluoroshield (AB104135)

  • 5
  • 1
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Product details

This product is manufactured by BioVision, an Abcam company and was previously called B1098 DAPI. B1098-5 is the same size as the 5 mg size of ab285390.

Protect from air and light.

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
It is important to note that this is air sensitive and impurities can occur as a result of air oxidation|Store in the dark
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Product protocols

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Publications (58)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 105:e70133 PubMed40490914

2025

Ferulic Acid Inhibits Partial Epithelial-Mesenchymal Transition in Renal Tubular Epithelial Cells and Mitigates Renal Interstitial Fibrosis by Regulating the HIF-1α/Twist Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Xu,Dongsheng Yao,Tianying Lan,Yurou Chen,Dongping Chen,Minghai Shao,Yijing Zhou,Chaoyang Ye,Di Huang

Molecular medicine (Cambridge, Mass.) 31:168 PubMed40325385

2025

Inhibition of TLR4 mitigates sensorineural hearing loss resulting from cochlear inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jintao Lou,Fan Wu,Wei Liu,Rui Hu,Wuhui He,Yisi Feng,Yan Huang,Jia Guo,Jingman Deng,Zhen Zhao,Zhigang Zhang,Yu Si

MedComm 6:e70138 PubMed40264576

2025

Integrating Multiplex Immunohistochemistry and Machine Learning for Glioma Subtyping and Prognosis Prediction.

Applications

Unspecified application

Species

Unspecified reactive species

Houshi Xu,Zhen Fan,Shan Jiang,Maoyuan Sun,Huihui Chai,Ruize Zhu,Xiaoyu Liu,Yue Wang,Jiawen Chen,Junji Wei,Ying Mao,Zhifeng Shi

CNS neuroscience & therapeutics 31:e70368 PubMed40202077

2025

TRPC4 Mediates Trigeminal Neuropathic Pain via Ca-ERK/P38-ATF2 Pathway in the Trigeminal Ganglion of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xinlong Ke,Huajing Cai,Fangla Luo,Xing Zheng,Qian Hu,Youfa Zhou,Yongjie Wang,Xiangnan Zhang,Yeru Chen,Gang Chen

Journal of nanobiotechnology 23:282 PubMed40197477

2025

Microenvironment-adaptive nanomedicine MXene promotes flap survival by inhibiting ROS cascade and endothelial pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ningning Yang,Rongrong Hua,Yingying Lai,Peijun Zhu,Jian Ding,Xianhui Ma,Gaoxiang Yu,Yiheng Xia,Chao Liang,Weiyang Gao,Zhouguang Wang,Hongyu Zhang,Liangliang Yang,Kailiang Zhou,Lu Ge

Stem cell research & therapy 16:148 PubMed40140882

2025

Exosomes derived from a mesenchymal-like endometrial regenerative cells ameliorate renal ischemia reperfusion injury through delivery of CD73.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Shao,Hong-da Wang,Shao-Hua Ren,Qiang Chen,Zhao-Bo Wang,Yi-Ni Xu,Tong Liu,Cheng-Lu Sun,Yi-Yi Xiao,Hong-Yu Jiang,Yi-Cheng Li,Peng-Yu Zhao,Guang-Mei Yang,Xu Liu,Yu-Fan Ren,Hao Wang

Frontiers in cellular and infection microbiology 14:1413018 PubMed40104260

2025

Significance of the gut tract in the therapeutic mechanisms of polydopamine for acute cerebral infarction: neuro-immune interaction through the gut-brain axis.

Applications

Unspecified application

Species

Unspecified reactive species

Feng-Hua Xu,Xiao Sun,Jun Zhu,Ling-Yang Kong,Yuan Chang,Ning Li,Wen-Xiang Hui,Cong-Peng Zhang,Yi-Ming Cheng,Wen-Xin Han,Zhi-Min Tian,Yan-Ning Qiao,Dong-Feng Chen,Lei Liu,Da-Yun Feng,Jing Han

International journal of medical sciences 22:1602-1611 PubMed40093806

2025

Escin Rescues Blood-brain Barrier and Inhibits NLRP3 Inflammasome-mediated Pyroptosis in Rats with Superior Sagital Sinus Thrombosis.

Applications

Unspecified application

Species

Unspecified reactive species

Min Li,Xinjian Liu,Xinyu Zhou,Ran Meng,Xunming Ji

European journal of medical research 30:144 PubMed40022250

2025

The mechanism of high mobility group box-1 in the proliferation and macrophage polarization in esophageal squamous cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liling Luo,Chao Jiang,Songxi Xie

Life metabolism 3:loae026 PubMed39873005

2025

Phytic acid-based nanomedicine against mTOR represses lipogenesis and immune response for metabolic dysfunction-associated steatohepatitis therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Fenghua Xu,Shoujie Zhao,Yejing Zhu,Jun Zhu,Lingyang Kong,Huichen Li,Shouzheng Ma,Bo Wang,Yongquan Qu,Zhimin Tian,Junlong Zhao,Lei Liu
View all publications
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