MW 432.5 Da, Purity >99%. γ-Secretase inhibitor. Inhibits Notch signalling in vitro. Inhibits total Aβ and Aβ42 production in human primary cultures (IC50 values are 115 and 200 nM, respectively) with no effect on APPα and APPβ levels. Blood-brain barrier permeable. 3D Growth matrix component and component of cerebral organoid differentiation media.
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- Cell 186:4676-4693.e292023Stepwise emergence of the neuronal gene expression program in early animal evolution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSebastián R Najle,Xavier Grau-Bové,Anamaria Elek,Cristina Navarrete,Damiano Cianferoni,Cristina Chiva,Didac Cañas-Armenteros,Arrate Mallabiabarrena,Kai Kamm,Eduard Sabidó,Harald Gruber-Vodicka,Bernd Schierwater,Luis Serrano,Arnau Sebé-PedrósPubMed 37729907
- Burns & trauma 11:tkad0322023Notch4 participates in mesenchymal stem cell-induced differentiation in 3D-printed matrix and is implicated in eccrine sweat gland morphogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuzhen Wang,Fanliang Zhang,Bin Yao,Linhao Hou,Zhao Li,Wei Song,Yi Kong,Yaxin Tan,Xiaobing Fu,Sha HuangPubMed 37397510
- Stem cell research & therapy 14:192023Generation of multilineage liver organoids with luminal vasculature and bile ducts from human pluripotent stem cells via modulation of Notch signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyo Jin Kim,Gyeongmin Kim,Kyun Yoo Chi,Hyemin Kim,Yu Jin Jang,Seongyea Jo,Jihun Lee,Youngseok Lee,Dong-Hun Woo,Choongseong Han,Sang Kyum Kim,Han-Jin Park,Jong-Hoon KimPubMed 36737811
- iScience 26:1059622023Distinct but interchangeable subpopulations of colorectal cancer cells with different growth fates and drug sensitivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberto Coppo,Jumpei Kondo,Keita Iida,Mariko Okada,Kunishige Onuma,Yoshihisa Tanaka,Mayumi Kamada,Masayuki Ohue,Kenji Kawada,Kazutaka Obama,Masahiro InouePubMed 36718360
- Current protocols 2:e3412022Stage-Specific Generation of Human Pluripotent Stem Cell Derived Lung Models to Measure CFTR Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuk Yee Ngan,Henry T Quach,Onofrio Laselva,Elena N Huang,Maria Mangos,Sunny Xia,Christine E Bear,Amy P WongPubMed 35025140
- Journal of experimental & clinical cancer research : CR 40:3252021M6A associated TSUC7 inhibition contributed to Erlotinib resistance in lung adenocarcinoma through a notch signaling activation dependent way.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Li,Zi-Yang Peng,Shan Gao,Qing-Shi Wang,Rui Wang,Xiang Li,Guo-Dong Xiao,Jing Zhang,Hong Ren,Shou-Ching Tang,Xin SunPubMed 34656164
- Experimental and therapeutic medicine 22:9722021Effect of the Notch4/Dll4 signaling pathway in early gestational intrauterine infection on lung development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCanyang Zhan,Yi Sun,Jiarong Pan,Lihua Chen,Tianming YuanPubMed 34335914
- STAR protocols 2:1004712021Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLay Shuen Tan,Resilind Su Ern Chew,Natasha Hui Jin Ng,Adrian Kee Keong TeoPubMed 33997805
- FEBS open bio 11:354-3662021Alpha-synuclein dynamics in induced pluripotent stem cell-derived dopaminergic neurons from a Parkinson's disease patient (PARK4) with SNCA triplication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHayato Fukusumi,Kazuyuki Togo,Miho Sumida,Masayuki Nakamori,Satoshi Obika,Kousuke Baba,Tomoko Shofuda,Daisuke Ito,Hideyuki Okano,Hideki Mochizuki,Yonehiro KanemuraPubMed 33301617
Key facts
- CAS number
- 208255-80-5
- Purity
- > 99%
- Form
- Solid
- Molecular weight
- 432.5 Da
- Molecular formula
- C23H26F2N2O4
- PubChem identifier
- 5311272
- Nature
- Synthetic
Target data
Alternative names
6530402N02Rik, AD3, AD3L, AD3LP, AD4, AD5, AL138795.3, APH 2, APH1A gamma secretase subunit, APH1A_HUMAN, ATAG1874, Ad3h, Alzheimer disease 4, Anterior Pharynx Defective 1, Anterior pharynx defective 1 homolog A, Anterior pharynx defective 2, Aph-1alpha, CGI 78, CMD1V, E5-1, FAD, Gamma Secretase Subunit PEN2, Gamma-secretase subunit APH-1A, Gamma-secretase subunit PEN-2, Hematopoietic stem/progenitor cells protein MDS033, Homo Sapiens Clone CC44 Senilin 1, KIAA0253, Likely ortholog of C. elegans anterior pharynx defective 1A, MDS033, MSTP064, NCT, NICA_HUMAN, Ncstn, Nicastrin, OTTHUMP00000035671, OTTHUMP00000035672, OTTHUMP00000228286, OTTHUMP00000228288, PEN2_HUMAN, PS-1, PS-2, PS1-CTF12, PSEN1, PSEN2, PSF, PSN1_HUMAN, PSN2_HUMAN, PSNL1, PSNL2, Presenilin 2, Presenilin 2 (Alzheimer disease 4), Presenilin Enhancer 2, Presenilin enhancer 2 homolog, Presenilin enhancer protein 2, Presenilin-1 CTF12, Presenilin-2 CTF subunit, Presenilin-stabilization factor, Protein S182, RP11 517F10.1, RP11517F101, S182, STM-2, UNQ579/PRO1141, psenen
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MW 432.5 Da, Purity >99%. γ-Secretase inhibitor. Inhibits Notch signalling in vitro. Inhibits total Aβ and Aβ42 production in human primary cultures (IC50 values are 115 and 200 nM, respectively) with no effect on APPα and APPβ levels. Blood-brain barrier permeable. 3D Growth matrix component and component of cerebral organoid differentiation media.
Key facts
- CAS number
- 208255-80-5
- Purity
- > 99%
- Form
- Solid
- Molecular weight
- 432.5 Da
- Molecular formula
- C23H26F2N2O4
- PubChem identifier
- 5311272
- Nature
- Synthetic
- Biochemical name
- Dapt
- Biological description
γ-Secretase inhibitor. Inhibits Notch signalling in vitro. Inhibits total Aβ and Aβ42 production in human primary cultures (IC50 values are 115 and 200 nM, respectively) with no effect on APPα and APPβ levels. Blood-brain barrier permeable. 3D Growth matrix component and component of cerebral organoid differentiation media.
- Canonical SMILES
- CC(C(=O)NC(C1=CC=CC=C1)C(=O)OC(C)(C)C)NC(=O)CC2=CC(=CC(=C2)F)F
- Isomeric SMILES
- C[C@@H](C(=O)N[C@@H](C1=CC=CC=C1)C(=O)OC(C)(C)C)NC(=O)CC2=CC(=CC(=C2)F)F
- InChI
- InChI=1S/C23H26F2N2O4/c1-14(26-19(28)12-15-10-17(24)13-18(25)11-15)21(29)27-20(16-8-6-5-7-9-16)22(30)31-23(2,3)4/h5-11,13-14,20H,12H2,1-4H3,(H,26,28)(H,27,29)/t14-,20-/m0/s1
- InChIKey
- DWJXYEABWRJFSP-XOBRGWDASA-N
- IUPAC name
- tert-butyl (2S)-2-[[(2S)-2-[[2-(3,5-difluorophenyl)acetyl]amino]propanoyl]amino]-2-phenylacetate
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- The product can be stored for up to 12 months
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3 product images
Chemical Structure - DAPT, gamma-Secretase inhibitor (ab120633)
2D chemical structure image of ab120633, DAPT, gamma-Secretase inhibitor
Functional Studies - DAPT, gamma-Secretase inhibitor (ab120633)
Anti-AKT1 (phospho S473) antibody [EP2109Y] ab81283 staining AKT1 (phospho S473) in MCF7 cells treated with DAPT (ab120633), by ICC/IF. Decrease in expression of AKT1 (phospho S473) correlates with increased concentration of DAPT, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120633 (DAPT) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-AKT1 (phospho S473) antibody [EP2109Y] ab81283 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This image is courtesy of a customer review submitted by a verified customerFunctional Studies - DAPT, gamma-Secretase inhibitor (ab120633)
Kc167 cells were treated with varying concentrations of DAPT (ab120633) in DMSO for 16hr at 25°C; DMSO only was used as the negative control. The cells were further incubated for 30 minutes with 4mM EGTA in PBS (in the presence of DAPT), and were then lysed for analysis. To measure Notch activity, Notch targets E(spl)mβ-HLH and E(spl)m3-HLH mRNA levels were assayed. Data shows the fold change of mRNA levels of E(spl)mβ-HLH and E(spl)m3-HLH under different conditions, normalised to DMSO treatment (negative control). Notch activation by EGTA is abrogated by treatment with DAPT.
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