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AB120743

Dexamethasone, anti-inflammatory glucocorticoid

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(3 Publications)

MW 392.5 Da, Purity >99%. Anti-inflammatory glucocorticoid (IC50 = 38 nM and Ki = 6.7 nM for the glucocorticoid receptor). Displays 7-fold higher affinity for glucocorticoid receptors than hydrocortisone. Long-term effects following oral administration. Used in the generation of liver organoids.
3 Images
Functional Studies - Dexamethasone, anti-inflammatory glucocorticoid (AB120743)
  • FuncS

Unknown

Functional Studies - Dexamethasone, anti-inflammatory glucocorticoid (AB120743)

ab75974 staining p57 Kip2 in HeLa cells treated with dexamethasone (ab120743), by ICC/IF. Increase in p57 Kip2 expression correlates with increased concentration of dexamethasone as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120743 (dexamethasone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab75974 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Functional Studies - Dexamethasone, anti-inflammatory glucocorticoid (AB120743)
  • FuncS

Unknown

Functional Studies - Dexamethasone, anti-inflammatory glucocorticoid (AB120743)

1e7 3T3-L1 cells were treated for 15 minutes with 1 µM dexamethasone (ab120743) and 1 µg x mL-1 insulin (ab123768) with the addition of 50 µM forskolin (ab120058), 0.5 mM IBMX (ab120840) or 0.1 mM 8-CPT-cAMP (ab120424), extracted according to the protocol. Lysates were diluted 50-fold, and tested in duplicates (+/-SD).

Chemical Structure - Dexamethasone, anti-inflammatory glucocorticoid (AB120743)
  • Chemical Structure

Lab

Chemical Structure - Dexamethasone, anti-inflammatory glucocorticoid (AB120743)

2D chemical structure image of ab120743, Dexamethasone, anti-inflammatory glucocorticoid

Key facts

CAS number

50-02-2

Purity

>99%

Form

Solid

form

Molecular weight

392.5 Da

Molecular formula

C<sub>2</sub><sub>2</sub>H<sub>2</sub><sub>9</sub>FO<sub>5</sub>

PubChem

5743

Nature

Synthetic

Biochemical name

Dexamethasone

Biological description

Anti-inflammatory glucocorticoid (IC50 = 38 nM and Ki = 6.7 nM for the glucocorticoid receptor). Displays 7-fold higher affinity for glucocorticoid receptors than hydrocortisone. Long-term effects following oral administration. Used in the generation of liver organoids.

Canonical smiles

CC1CC2C3CCC4=CC(=O)C=CC4(C3(C(CC2(C1(C(=O)CO)O)C)O)F)C

Isomeric smiles

C[C@@H]1C[C@H]2[C@@H]3CCC4=CC(=O)C=C[C@@]4([C@]3([C@H](C[C@@]2([C@]1(C(=O)CO)O)C)O)F)C

InChi

InChI=1S/C22H29FO5/c1-12-8-16-15-5-4-13-9-14(25)6-7-19(13,2)21(15,23)17(26)10-20(16,3)22(12,28)18(27)11-24/h6-7,9,12,15-17,24,26,28H,4-5,8,10-11H2,1-3H3/t12-,15+,16+,17+,19+,20+,21+,22+/m1/s1

InChiKey

UREBDLICKHMUKA-CXSFZGCWSA-N

IUPAC Name

(8S,9R,10S,11S,13S,14S,16R,17R)-9-fluoro-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13,16-trimethyl-6,7,8,11,12,14,15,16-octahydrocyclopenta[a]phenanthren-3-one

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Complementary Products

Biochemicals

AB120440

NECA, adenosine agonist

Chemical Structure - NECA, adenosine agonist (AB120440)

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  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
It is important to note that this product is reported to be light sensitive|Store in the dark|Store under desiccating conditions
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Product protocols

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:595 PubMed40240542

2025

Acoustic modulation of mechanosensitive genes and adipocyte differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiro Kumeta,Makoto Otani,Masahiro Toyoda,Shige H Yoshimura

Acta pharmaceutica Sinica. B 12:1899-1912 PubMed35847503

2022

Disruption of adipocyte HIF-1 improves atherosclerosis through the inhibition of ceramide generation.

Applications

Unspecified application

Species

Unspecified reactive species

Pengcheng Wang,Guangyi Zeng,Yu Yan,Song-Yang Zhang,Yongqiang Dong,Yangming Zhang,Xingzhong Zhang,Huiying Liu,Zhipeng Zhang,Changtao Jiang,Yanli Pang

Clinical immunology (Orlando, Fla.) 155:126-135 PubMed25236785

2014

Increased ILC2s in the eosinophilic nasal polyp endotype are associated with corticosteroid responsiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah H Walford,Sean J Lund,Rachel E Baum,Andrew A White,Christopher M Bergeron,Jacob Husseman,Kelly J Bethel,David R Scott,Naseem Khorram,Marina Miller,David H Broide,Taylor A Doherty
View all publications
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