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AB145260

Dihydrorhodamine 123, Fluorescent probe for ROS

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MW 346.4 Da, Purity >98%. Cell-permeable fluorescent probe for ROS. Redox sensor. Detects presence of peroxynitrite. Fluoresces brighter extracellularly than Dihydrofluorescein diacetate (ab145351) but less bight intracellularly.
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Chemical Structure - Dihydrorhodamine 123, Fluorescent probe for ROS (AB145260)
  • Chemical Structure

Unknown

Chemical Structure - Dihydrorhodamine 123, Fluorescent probe for ROS (AB145260)

Chemical structure of ab145260, Dihydrorhodamine 123, Fluorescent probe for ROS

Key facts

CAS number

109244-58-8

Purity

>98%

Form

Solid

form

Molecular weight

346.4 Da

Molecular formula

C<sub>2</sub><sub>1</sub>H<sub>1</sub><sub>8</sub>N<sub>2</sub>O<sub>3</sub>

PubChem

105032

Nature

Synthetic

Solubility

Soluble in ethanol to 25 mM

Soluble in DMSO to 25 mM

Biochemical name

Dihydrorhodamine 123

Biological description

Cell-permeable fluorescent probe for ROS. Redox sensor. Detects presence of peroxynitrite. Fluoresces brighter extracellularly than Dihydrofluorescein diacetate (ab145351) but less bight intracellularly.

Canonical smiles

COC(=O)C1=CC=CC=C1C2C3=C(C=C(C=C3)N)OC4=C2C=CC(=C4)N

InChi

InChI=1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3

InChiKey

FNEZBBILNYNQGC-UHFFFAOYSA-N

IUPAC Name

methyl 2-(3,6-diamino-9H-xanthen-9-yl)benzoate

Excitation/Emission

Ex: 500nm, Em: 536nm

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Complementary Products

Biochemicals

AB145351

Dihydrofluorescein diacetate, Fluorescent intracellular oxidant probe

Chemical Structure - Dihydrofluorescein diacetate, Fluorescent intracellular oxidant probe (AB145351)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB238535

DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates)

Functional Studies - DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) (AB238535)

  • 4
  • 1
Assay Kits

AB113851

DCFDA / H2DCFDA - Cellular ROS Assay Kit

Cellular Activity - DCFDA / H2DCFDA - Cellular ROS Assay Kit (AB113851)

  • 5
  • 11
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Product promise

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