Dopamine hydrochloride, Endogenous neurotransmitter
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(1 Publication)
- FuncS
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Functional Studies - Dopamine hydrochloride, Endogenous neurotransmitter (AB120565)
ab32088 staining MEK1 (phospho S218 + S222) in SKNSH cells treated with dopamine hydrochloride (ab120565), by ICC/IF. Increase in MEK1 (phospho S218 + S222) expression correlates with increased concentration of dopamine hydrochloride, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120565 (dopamine hydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32088 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
- Chemical Structure
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Chemical Structure - Dopamine hydrochloride, Endogenous neurotransmitter (AB120565)
2D chemical structure image of ab120565, Dopamine hydrochloride, Endogenous neurotransmitter
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Publications (1)
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Scientific reports 10:20203 PubMed33214601
2020
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