Dynasore, dynamin inhibitor

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Dynasore, dynamin inhibitor (AB120192)

ab66705 staining PAI1 in HeLa cells treated with dynasore (ab120192), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynasore, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120689 (dynasore) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight^® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

• WB

PubMed

Western blot - Dynasore, dynamin inhibitor (AB120192)

Western blots of indicated proteins after knockdown with gene-specific siRNA against AP2M1 (ab120192) or non-targeting siRNA transfection control (NT).

siRNAs were transfected into HMVEC-L at the concentration of 100 nM for 48h. The cells were then infected with ANDV (MOI = 0.5) for 24 h or 48 h. Western blots were performed post infection to ensure knockdown of the specific protein expression. The blots were also probed with β-actin specific antibody as the gel-loading control.

Jens H et al. PLoS One. 2016; 11(10) : e0164768. doi : 10.1371/journal.pone.0164768

false

Jens H et al. PLoS One. 2016; 11(10): e0164768. doi: 10.1371/journal.pone.0164768 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

• Chemical Structure

Lab

Chemical Structure - Dynasore, dynamin inhibitor (AB120192)

2D chemical structure image of ab120192, Dynasore, dynamin inhibitor