Dyngo® 4a highly potent dynamin inhibitor

• Chemical Structure

Lab

Chemical Structure - Dyngo® 4a highly potent dynamin inhibitor (AB120689)

2D chemical structure image of ab120689, Dyngo® 4a, Novel, highly potentdynamin inhibitor

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Dyngo® 4a highly potent dynamin inhibitor (AB120689)

ab66705 staining PAI1 in HeLa cells treated with dyngo-4a™ (ab120689), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dyngo-4a™, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120689 (dyngo-4a™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight^® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

• FuncS

PubMed

Functional Studies - Dyngo® 4a highly potent dynamin inhibitor (AB120689)

Primary human monocyte-derived macrophages were treated without (Ctrl) and with ab120689 (Dyngo 4A) for 2 h. Effects on α-tubulin and acetylated tubulin (Ac-tubulin) were determined by confocal microscopy.

Credit : Kockx M et al. PLoS One. 2014; 9(10) : e111186. doi : 10.1371/journal.pone.0111186

Kockx M et al. PLoS One. 2014; 9(10): e111186. doi: 10.1371/journal.pone.0111186 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/