MW 338.45 g/mol, Purity >97%.
Negative control molecule for use with Dynole® 34-2 ab120463. Displays no significant activity at dynamin I or II (up to 300 µM).
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- Science signaling 11:2018Contact inhibitory Eph signaling suppresses EGF-promoted cell migration by decoupling EGFR activity from vesicular recycling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWayne Stallaert et. al.PubMed 30065026
- Scientific reports 7:45802017Dynamin-2 mutations linked to Centronuclear Myopathy impair actin-dependent trafficking in muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArlek M González-Jamett et. al.PubMed 28676641
- PloS one 12:e01724432017Dynamin2 controls Rap1 activation and integrin clustering in human T lymphocyte adhesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFelix J Eppler et. al.PubMed 28273099
- Cellular signalling 28:160-712015Impaired dynamin 2 function leads to increased AP-1 transcriptional activity through the JNK/c-Jun pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEwelina Szymanska,Agnieszka Skowronek,Marta MiaczynskaPubMed 26475677
- PLoS pathogens 9:e10033092013Host cell entry of respiratory syncytial virus involves macropinocytosis followed by proteolytic activation of the F protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMagdalena Anna Krzyzaniak et. al.PubMed 23593008
- Retrovirology 8:992011Inhibition of HIV-1 endocytosis allows lipid mixing at the plasma membrane, but not complete fusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichelle de la Vega et. al.PubMed 22145853
- The Biochemical journal 440:185-932011Intracellular curvature-generating proteins in cell-to-cell fusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJean-Philippe Richard,Evgenia Leikina,Ralf Langen,William Mike Henne,Margarita Popova,Tamas Balla,Harvey T McMahon,Michael M Kozlov,Leonid V ChernomordikPubMed 21895608
Key facts
- Purity
- > 97%
- Form
- Solid
- Molecular weight
- 338.45 g/mol
- Nature
- Synthetic
Recommended products
MW 338.45 g/mol, Purity >97%.
Negative control molecule for use with Dynole® 34-2 ab120463. Displays no significant activity at dynamin I or II (up to 300 µM).
Key facts
- Purity
- > 97%
- Form
- Solid
- Molecular weight
- 338.45 g/mol
- Nature
- Synthetic
- Solubility
Soluble in DMSO to 100 mM.
- Biological description
Negative control molecule for use with Dynole® 34-2 ab120463. Displays no significant activity at dynamin I or II (up to 300 µM).
- Canonical SMILES
- CCCNC(=O)C(C#N)=CC2CN(CCCN(C)C)C1CCCCC12
- InChI
- InChI=1S/C20H26N4O/c1-4-10-22-20(25)16(14-21)13-17-15-24(12-7-11-23(2)3)19-9-6-5-8-18(17)19/h5-6,8-9,13,15H,4,7,10-12H2,1-3H3,(H,22,25)/b16-13+
- InChIKey
- NOOFIXCUBMYDIB-DTQAZKPQSA-N
- IUPAC name
- 2-Cyano-N-propyl-3-[1-(3-dimethylaminopropyl)-1H-indol-3-yl]acrylamide
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
Notes
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole® is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.
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3 product images
Chemical Structure - Dynole® 31-2, Negative control for Dynolereg 34-2 (ab120464)
2D chemical structure image of ab120464, Dynole® 31-2, Negative control for Dynolereg 34-2
Immunocytochemistry/ Immunofluorescence - Dynole® 31-2, Negative control for Dynolereg 34-2 (ab120464)
Anti-PAI1 antibody ab66705 staining PAI1 in HeLa cells treated with dynole-31-2™ (ab120464), by ICC/IF. No change in PAI1 expression with increased concentration of dynole-31-2™ (negative control for dynole 34-2™ (Dynole® 34-2, dynamin I and dynamin II inhibitor ab120463), as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120464 (dynole-31-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-PAI1 antibody ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Eppler et al PLoS One. 2017; 12(3): e0172443. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Dynole® 31-2, Negative control for Dynolereg 34-2 (ab120464)
Dynamin2 regulates integrin-mediated T lymphocyte adhesion under laminar flow.End point quantification of T lymphocyte adhesion following treatment of the cells with either Dynole 31–2 as a control or Dynole 34–2 to inhibit dynamin2 activity (n = 6)
(From Figure 2D of Eppler et al).
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