Dynole® 31-2, Negative control for Dynolereg 34-2

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Dynole® 31-2, Negative control for Dynolereg 34-2 (AB120464)

ab66705 staining PAI1 in HeLa cells treated with dynole-31-2™ (ab120464), by ICC/IF. No change in PAI1 expression with increased concentration of dynole-31-2™ (negative control for dynole 34-2™ (ab120463), as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120464 (dynole-31-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight^® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

• FuncS

PubMed

Functional Studies - Dynole® 31-2, Negative control for Dynolereg 34-2 (AB120464)

Dynamin2 regulates integrin-mediated T lymphocyte adhesion under laminar flow.

End point quantification of T lymphocyte adhesion following treatment of the cells with either Dynole 31–2 as a control or Dynole 34–2 to inhibit dynamin2 activity (n = 6)

(From Figure 2D of Eppler et al).

Eppler et al PLoS One. 2017; 12(3): e0172443. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• Chemical Structure

Lab

Chemical Structure - Dynole® 31-2, Negative control for Dynolereg 34-2 (AB120464)

2D chemical structure image of ab120464, Dynole® 31-2, Negative control for Dynolereg 34-2