JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB146186

EdU, DNA synthesis monitoring probe

Be the first to review this product! Submit a review

|

(25 Publications)

Thymidine analog. Alternative to BrdU (5-Bromo-2'-deoxyuridine) (ab142567) for measuring de novo DNA synthesis during S-phase. MW 252.22, Purity >98%.

- Used for probing short-term DNA synthesis in tissue culture and living organisms
- Available in different sizes to fit your experimental needs
1 Images
Chemical Structure - EdU, DNA synthesis monitoring probe (AB146186)
  • Chemical Structure

Lab

Chemical Structure - EdU, DNA synthesis monitoring probe (AB146186)

2D chemical structure image of ab146186, EdU, DNA synthesis monitoring probe

Key facts

CAS number

61135-33-9

Purity

>98%

Form

Solid

form

Molecular weight

252.22 Da

Molecular formula

C<sub>1</sub><sub>1</sub>H<sub>1</sub><sub>2</sub>N<sub>2</sub>O<sub>5</sub>

PubChem

472172

Nature

Synthetic

Solubility

Soluble in water to 10 mM

Soluble in DMSO to 100 mM

Biochemical name

5-Ethynyl-2'-deoxyuridine

Biological description

Thymidine analog. Alternative to BrdU (5-Bromo-2'-deoxyuridine) (ab142567) for measuring de novo DNA synthesis during S-phase. Cytotoxic anti-metabolite. Used for probing short-term DNA synthesis in tissue culture and living organisms where prolonged cell survival is not required.

Canonical smiles

C#CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)O

Isomeric smiles

C#CC1=CN(C(=O)NC1=O)[C@H]2C[C@@H]([C@H](O2)CO)O

InChi

InChI=1S/C11H12N2O5/c1-2-6-4-13(11(17)12-10(6)16)9-3-7(15)8(5-14)18-9/h1,4,7-9,14-15H,3,5H2,(H,12,16,17)/t7-,8+,9+/m0/s1

InChiKey

CDEURGJCGCHYFH-DJLDLDEBSA-N

IUPAC Name

5-ethynyl-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione

style
left-column
style
left-column

Product details

Check out our range of DNA synthesis biochemicals here
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3474 PubMed40216785

2025

Suppression of TGF-β/SMAD signaling by an inner nuclear membrane phosphatase complex.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Ji,Wing-Yan Skyla Siu,Maria Emilia Dueñas,Leonie Müller,Matthias Trost,Pedro Carvalho

eLife 13: PubMed40178080

2025

Simultaneous cyclin D1 overexpression and p27 knockdown enable robust Müller glia cell cycle reactivation in uninjured mouse retina.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifei Wu,Baoshan Liao,Julia Ying,Jan Keung,Zongli Zheng,Virpi Ahola,Wenjun Xiong

Cell reports 44:115259 PubMed39893637

2025

The homologous recombination factors BRCA2 and PALB2 interplay with mismatch repair pathways to maintain centromere stability and cell viability.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Graham,Lucia Rampazzo,Chin Wei Brian Leung,Jacob Wall,Emőke Zsanett Gerőcz,Mikhail Liskovykh,Nikolay Goncharov,Xanita Saayman,Ramazan Gundogdu,Masato T Kanemaki,Hiroshi Masumoto,Vladimir Larionov,Natalay Kouprina,Fumiko Esashi

PLoS genetics 20:e1011197 PubMed39012914

2024

Meiotic double-strand break repair DNA synthesis tracts in Arabidopsis thaliana.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Hernández Sánchez-Rebato,Veit Schubert,Charles I White

Science advances 10:eadn2091 PubMed38996013

2024

Robust reprogramming of glia into neurons by inhibition of Notch signaling and nuclear factor I (NFI) factors in adult mammalian retina.

Applications

Unspecified application

Species

Unspecified reactive species

Nguyet Le,Trieu-Duc Vu,Isabella Palazzo,Ritvik Pulya,Yehna Kim,Seth Blackshaw,Thanh Hoang

Science advances 10:eadj2020 PubMed38924411

2024

corrects aberrant matrix metalloproteinase expression to promote reepithelialization of diabetic wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Ellen K White,Aayushi Uberoi,Jamie Ting-Chun Pan,Jordan T Ort,Amy E Campbell,Sofia M Murga-Garrido,Jordan C Harris,Preeti Bhanap,Monica Wei,Nelida Y Robles,Sue E Gardner,Elizabeth A Grice

Cell metabolism 35:472-486.e6 PubMed36854304

2023

Multiomics reveals glutathione metabolism as a driver of bimodality during stem cell aging.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel I Benjamin,Jamie O Brett,Pieter Both,Joel S Benjamin,Heather L Ishak,Jengmin Kang,Soochi Kim,Mingyu Chung,Marina Arjona,Christopher W Nutter,Jenna H Tan,Ananya K Krishnan,Hunter Dulay,Sharon M Louie,Antoine de Morree,Daniel K Nomura,Thomas A Rando

iScience 25:105302 PubMed36304107

2022

Hypothesis-generating proteome perturbation to identify NEU-4438 and acoziborole modes of action in the African Trypanosome.

Applications

Unspecified application

Species

Unspecified reactive species

Amrita Sharma,Michael Cipriano,Lori Ferrins,Stephen L Hajduk,Kojo Mensa-Wilmot

Development (Cambridge, England) 149: PubMed35833708

2022

Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyi Mai,Xiaoxuan Zhu,Esther Yi Ching Wan,Shengyu Wu,Jesslyn Nagalin Yonathan,Jun Wang,Ying Li,Jessica Yuen Wuen Ma,Bing Zuo,Dennis Yan-Yin Tse,Pui-Chi Lo,Xin Wang,Kui Ming Chan,David M Wu,Wenjun Xiong

Development (Cambridge, England) : PubMed35831947

2022

Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyi Mai,Xiaoxuan Zhu,Esther Yi Ching Wan,Shengyu Wu,Jesslyn Nagalin Yonathan,Jun Wang,Ying Li,Jessica Yuen Wuen Ma,Bing Zuo,Dennis Yan-Yin Tse,Pui-Chi Lo,Xin Wang,Kui Ming Chan,David M Wu,Wenjun Xiong
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB222421

EdU Assay / EdU Staining Proliferation Kit (iFluor 647)

assay-kits

edu-assay-edu-staining-proliferation-kit-ifluor-647-ab222421

5

(1 reviews)

Alternative Product
Assay Kits

AB219801

EdU Assay / EdU Staining Proliferation Kit (iFluor 488)

assay-kits

edu-assay-edu-staining-proliferation-kit-ifluor-488-ab219801

5

(1 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com