Elastase Substrate, Fluorogenic (MeOSuc-AAPV-AFC)

Key facts

CAS number

312-84-5

Purity

>99%

Molecular weight

681.67 g/mol

Molecular formula

C₃₁H₃₈F₃N₅O₉

Nature

Synthetic

Solubility

Soluble in DMSO

Biological description

Highly sensitive coumarin-fluorogenic substrate for neutrophil elastase (K_m =130 μM for leukocyte elastase). Compatible with in vitro assays with purified enzyme, on live cells, or in conditioned medium. The excitation wavelength is 380 nm and the emission wavelength is 500 nm.

Canonical smiles

O=C(OC)CCC(=O)NC(C)C(=O)NC(C)C(=O)N1CCCC1C(=O)NC(C(C)C)C(=O)NC2CCC3C(C2)OC(=O)C=C3C(F)(F)F

InChi

InChI=1S/C31H38F3N5O9/c1-15(2)26(29(45)37-18-8-9-19-20(31(32,33)34)14-25(42)48-22(19)13-18)38-28(44)21-7-6-12-39(21)30(46)17(4)36-27(43)16(3)35-23(40)10-11-24(41)47-5/h8-9,13-17,21,26H,6-7,10-12H2,1-5H3,(H,35,40)(H,36,43)(H,37,45)(H,38,44)40)(H,34,42)(H,35,41)

InChiKey

FLFLPZJVJVKNIW-UHFFFAOYSA-N

IUPAC Name

N-(Methoxysuccinyl)-Ala-Ala-Pro-Val-7-amino-4-trifluormethylcoumarin