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AB145323

Ethidium homodimer, Fluorimetric detection reagent

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(4 Publications )

MW 856.75 g/mol, Purity 90%. High-affinity nucleic acid stain for ssDNA, dsDNA, RNA, oligonucleotides and triplex DNA. Weakly fluorescent until bound to DNA. It does not cross intact cell membranes and can be used to test cell viability.
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Chemical Structure - Ethidium homodimer, Fluorimetric detection reagent (AB145323)
  • Chemical Structure

Lab

Chemical Structure - Ethidium homodimer, Fluorimetric detection reagent (AB145323)

2D chemical structure image of ab145323, Ethidium homodimer, Fluorimetric detection reagent

Key facts

CAS number

61926-22-5

Purity

90%

Form

Solid

form

Molecular weight

856.75 g/mol

Molecular formula

C<sub>4</sub><sub>6</sub>H<sub>5</sub><sub>0</sub>Cl<sub>4</sub>N<sub>8</sub>

Nature

Synthetic

Solubility

Soluble in water

Soluble in DMSO

Biological description

High-affinity nucleic acid stain for ssDNA, dsDNA, RNA, oligonucleotides and triplex DNA. Weakly fluorescent until bound to DNA. It does not cross intact cell membranes and can be used to test cell viability.

InChi

InChI=1S/C46H46N8.2ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;2*1H

InChiKey

BGWLYQZDNFIFRX-UHFFFAOYSA-N

Excitation/Emission

Ex: 528nm, Em: 617nm

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Complementary Products

Secondary Antibodies

AB150115

Goat Anti-Mouse IgG H&L (Alexa Fluor® 647)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (AB150115)

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Biochemicals

AB141420

Calcein AM, fluorescent dye for cell viability

Chemical Structure - Calcein AM, fluorescent dye for cell viability (AB141420)

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Biochemicals

AB217298

ABT-199 (Venetoclax), Bcl-2 inhibitor.

Chemical Structure - ABT-199 (Venetoclax), Bcl-2 inhibitor. (AB217298)

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
Handling instructions

Need more advice on solubility, usage and handling? Please visit our our product support page for more details.

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Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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Refer to SDS for further information.

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Product protocols

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Gels (Basel, Switzerland) 10: PubMed39590101

2024

In Vitro Investigation of 3D Printed Hydrogel Scaffolds with Electrospun Tidemark Component for Modeling Osteochondral Interface.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria Effiong Effanga,Dana Akilbekova,Fariza Mukasheva,Xiao Zhao,Dilhan M Kalyon,Cevat Erisken

Frontiers in bioengineering and biotechnology 10:884071 PubMed35519615

2022

Engineering Vascularized Islet Macroencapsulation Devices: An Platform to Study Oxygen Transport in Perfused Immobilized Pancreatic Beta Cell Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Fernandez S A,Champion K S,Danielczak L,Gasparrini M,Paraskevas S,Leask R L,Hoesli C A

PloS one 16:e0252707 PubMed34133443

2021

Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Gizem Ece Derici,Sibel Özdaş,İpek Canatar,Murat Koç

Stem cell research & therapy 12:83 PubMed33494791

2021

Glucocorticoid agonists enhance retinal stem cell self-renewal and proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth N Grisé,Nelson X Bautista,Krystal Jacques,Brenda L K Coles,Derek van der Kooy
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Product promise

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