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AB120081

FCCP, mitochondrial oxidative phosphorylation uncoupler

4

(1 Review)

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(54 Publications)

FCCP (ab120081) is a potent mitochondrial oxidative phosphorylation (OXPHOS) uncoupler. Molecular weight (MW): 254.17; Purity > 99%.

- Proven performance: cited in over 55 publications
- Soluble in DMSO to 100 mM
- Available in different sizes to fit your experimental needs
2 Images
Chemical Structure - FCCP, mitochondrial oxidative phosphorylation uncoupler (AB120081)
  • Chemical Structure

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Chemical Structure - FCCP, mitochondrial oxidative phosphorylation uncoupler (AB120081)

2D chemical structure image of ab120081, FCCP, mitochondrial oxidative phosphorylation uncoupler

Cellular Activation - FCCP, mitochondrial oxidative phosphorylation uncoupler (AB120081)
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Unknown

Cellular Activation - FCCP, mitochondrial oxidative phosphorylation uncoupler (AB120081)

HT29 cells were treated with the indicated compounds ((etoposide, 10 µM; gemcitabine 0.1 µM; paclitaxel 0.01 µM; PD901 1 µM, VX-680 0.2 µM) for 24 hours before analysis of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) using the Seahorse XF96 extracellular flux analyzer. Baseline rates (black) were determined at the indicated times before the addition of oligomycin (green) and then FCCP (red). Rate data are normalized to per-well cell number determined by post-analysis high-content imaging.

Image from Chan G K Y, et al. Plos One, 8(5), e63583. Fig S4,; doi: 10.1371/journal.pone.0063583

Key facts

CAS number

370-86-5

Purity

>99%

Form

Solid

form

Molecular weight

254.17 Da

Molecular formula

C<sub>1</sub><sub>0</sub>H<sub>5</sub>F<sub>3</sub>N<sub>4</sub>O

PubChem

3330

Nature

Synthetic

Solubility

Soluble in DMSO to 100 mM

Biochemical name

Carbonyl cyanide p-trifluoromethoxyphenylhydrazone

Canonical smiles

C1=CC(=CC=C1NN=C(C#N)C#N)OC(F)(F)F

InChi

InChI=1S/C10H5F3N4O/c11-10(12,13)18-9-3-1-7(2-4-9)16-17-8(5-14)6-15/h1-4,16H

IUPAC Name

2-[[4-(trifluoromethoxy)phenyl]hydrazinylidene]propanedinitrile

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Puromycin dihydrochloride, Protein biosynthesis inhibitor

Chemical Structure - Puromycin dihydrochloride, Protein biosynthesis inhibitor (AB141453)

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Assay Kits

AB113852

TMRE-Mitochondrial Membrane Potential Assay Kit

Functional Studies - TMRE-Mitochondrial Membrane Potential Assay Kit (AB113852)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Biochemicals

AB141229

CCCP, Mitochondrial oxidative phosphorylation uncoupler

Immunocytochemistry/ Immunofluorescence - CCCP, Mitochondrial oxidative phosphorylation uncoupler (AB141229)

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Publications (54)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5377 PubMed40562775

2025

Metabolic reprogramming of the neovascular niche promotes regenerative angiogenesis in proliferative retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Gael Cagnone,Sheetal Pundir,Charlotte Betus,Tapan Agnihotri,Anli Ren,Jin Sung -Kim,Noémie-Rose Harvey,Emilie Heckel,Mei Xi Chen,Anu Situ,Perrine Gaub,Nicholas Kim,Ashim Das,Severine Leclerc,Florian Wünnemann,Louis Berillon,Gregor Andelfinger,Sergio Crespo-Garcia,Alexandre Dubrac,Flavio A Rezende,Clary B Clish,Bruno Maranda,José Carlos Rivera,Lois E H Smith,Przemyslaw Sapieha,Jean-Sébastien Joyal

Biomedicines 13: PubMed39857691

2025

Germline Single-Nucleotide Polymorphism Causes Alterations in Mitochondrial Metabolism and Leads to Increased ROS-Mediated DNA Damage in a Murine Model of Human Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Vorwerk,Longlong Liu,Theresa Helene Stadler,Daria Frank,Helal Mohammed Mohammed Ahmed,Pradeep Kumar Patnana,Maxim Kebenko,Eva Dazert,Bertram Opalka,Nikolas von Bubnoff,Cyrus Khandanpour

The EMBO journal 43:5972-6000 PubMed39448884

2024

Cyclophilin D plays a critical role in the survival of senescent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Margherita Protasoni,Vanessa López-Polo,Camille Stephan-Otto Attolini,Julian Brandariz,Nicolas Herranz,Joaquin Mateo,Sergio Ruiz,Oscar Fernandez-Capetillo,Marta Kovatcheva,Manuel Serrano

Journal of nanobiotechnology 22:540 PubMed39237942

2024

Enhanced osteogenic differentiation in 3D hydrogel scaffold via macrophage mitochondrial transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Shui Qiu,Lili Cao,Dingding Xiang,Shu Wang,Di Wang,Yiyi Qian,Xiaohua Li,Xiaoshu Zhou

International journal of biological sciences 20:2860-2880 PubMed38904024

2024

Activation of Neurotoxic Astrocytes Due to Mitochondrial Dysfunction Triggered by Mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Xiao Liang,Anbin Chen,Atefeh Kianian,Cecilie Katrin Kristiansen,Tsering Yangzom,Jessica Furriol,Lena Elise Høyland,Mathias Ziegler,Torbjørn Kråkenes,Charalampos Tzoulis,Evandro Fei Fang,Gareth John Sullivan,Laurence A Bindoff

PloS one 19:e0298697 PubMed38536780

2024

THC and sperm: Impact on fertilization capability, pre-implantation in vitro development and epigenetic modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander G Kuzma-Hunt,Reem Sabry,Ola S Davis,Vivien B Truong,Jibran Y Khokhar,Laura A Favetta

Cell death & disease 15:203 PubMed38467609

2024

Genome-wide CRISPR/Cas9 screen shows that loss of GET4 increases mitochondria-endoplasmic reticulum contact sites and is neuroprotective.

Applications

Unspecified application

Species

Unspecified reactive species

Emma L Wilson,Yizhou Yu,Nuno S Leal,James A Woodward,Nikolaos Patikas,Jordan L Morris,Sarah F Field,William Plumbly,Vincent Paupe,Suvagata R Chowdhury,Robin Antrobus,Georgina E Lindop,Yusuf M Adia,Samantha H Y Loh,Julien Prudent,L Miguel Martins,Emmanouil Metzakopian

Genetics in medicine : official journal of the American College of Medical Genetics 26:101117 PubMed38459834

2024

Alternative splicing expands the clinical spectrum of NDUFS6-related mitochondrial disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Camila Armirola-Ricaurte,Noortje Zonnekein,Georgios Koutsis,Silvia Amor-Barris,Ana Lara Pelayo-Negro,Derek Atkinson,Stephanie Efthymiou,Valentina Turchetti,Argyris Dinopoulos,Antonio Garcia,Mert Karakaya,German Moris,Ayşe Ipek Polat,Uluç Yiş,Carmen Espinos,Liedewei Van de Vondel,Els De Vriendt,Georgia Karadima,Brunhilde Wirth,Michael Hanna,Henry Houlden,Jose Berciano,Albena Jordanova

NPJ Parkinson's disease 10:49 PubMed38429321

2024

G2019S selective LRRK2 kinase inhibitor abrogates mitochondrial DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Pena,Tara Richbourg,Claudia P Gonzalez-Hunt,Rui Qi,Paul Wren,Carrolee Barlow,Natalie F Shanks,Holly J Carlisle,Laurie H Sanders

Gastroenterology 166:826-841.e19 PubMed38266738

2024

Interleukin 21 Drives a Hypermetabolic State and CD4 T-Cell-Associated Pathogenicity in Chronic Intestinal Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Adebowale O Bamidele,Shravan K Mishra,Guilherme Piovezani Ramos,Petra Hirsova,Emily E Klatt,Leena M Abdelrahman,Mary R Sagstetter,Heidi M Davidson,Patrick J Fehrenbach,Lucía Valenzuela-Pérez,Hyun Se Kim Lee,Song Zhang,Abner Aguirre Lopez,Ahmed T Kurdi,Maria S Westphal,Michelle M Gonzalez,Joseph M Gaballa,Robyn Laura Kosinsky,Hee Eun Lee,Thomas C Smyrk,Glenn Bantug,Naomi M Gades,William A Faubion
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