MW 353.68 Da, Purity >98%. Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent DAT and VMAT2 substrate. Enables two-photon microscopic imaging of localization and activity of dopaminergic presynaptic terminals in the striatum of mouse acute brain slice. More selective for dopaminergic synapses than FFN511 (ab120331).
Exhibits greater fluorescence emission in neutral than acidic environments allowing optical measurement of synaptic vesicle content release.
Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags.
Images
Publications
Filter by
- NPJ Parkinson's disease 8:1622022Protein aggregation and calcium dysregulation are hallmarks of familial Parkinson's disease in midbrain dopaminergic neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGurvir S Virdi,Minee L Choi,James R Evans,Zhi Yao,Dilan Athauda,Stephanie Strohbuecker,Raja S Nirujogi,Anna I Wernick,Noelia Pelegrina-Hidalgo,Craig Leighton,Rebecca S Saleeb,Olga Kopach,Haya Alrashidi,Daniela Melandri,Jimena Perez-Lloret,Plamena R Angelova,Sergiy Sylantyev,Simon Eaton,Simon Heales,Dmitri A Rusakov,Dario R Alessi,Tilo Kunath,Mathew H Horrocks,Andrey Y Abramov,Rickie Patani,Sonia GandhiPubMed 36424392
- Frontiers in cellular neuroscience 9:3892015Vesicular expression and release of ATP from dopaminergic neurons of the mouse retina and midbrain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTracy Ho,Andrew I Jobling,Ursula Greferath,Trinette Chuang,Archana Ramesh,Erica L Fletcher,Kirstan A VesseyPubMed 26500494
- Proceedings of the National Academy of Sciences of the United States of America 110:870-52013Fluorescent dopamine tracer resolves individual dopaminergic synapses and their activity in the brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPamela C Rodriguez,Daniela B Pereira,Anders Borgkvist,Minerva Y Wong,Candace Barnard,Mark S Sonders,Hui Zhang,Dalibor Sames,David SulzerPubMed 23277566
Key facts
- CAS number
- 1234064-11-9
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 353.68 Da
- Molecular formula
- C13H11ClF3NO5
- PubChem identifier
- 91885430
- Nature
- Synthetic
Target data
Recommended products
MW 353.68 Da, Purity >98%. Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent DAT and VMAT2 substrate. Enables two-photon microscopic imaging of localization and activity of dopaminergic presynaptic terminals in the striatum of mouse acute brain slice. More selective for dopaminergic synapses than FFN511 (ab120331).
Exhibits greater fluorescence emission in neutral than acidic environments allowing optical measurement of synaptic vesicle content release.
Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags.
Key facts
- CAS number
- 1234064-11-9
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 353.68 Da
- Molecular formula
- C13H11ClF3NO5
- PubChem identifier
- 91885430
- Nature
- Synthetic
- Solubility
Soluble in water to 50 mM.
- Biochemical name
- 4-(2-Aminoethyl)-6-chloro-7-hydroxychromen-2-one;2,2,2-trifluoroacetic acid
- Biological description
Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent DAT and VMAT2 substrate. Enables two-photon microscopic imaging of localization and activity of dopaminergic presynaptic terminals in the striatum of mouse acute brain slice. More selective for dopaminergic synapses than FFN511 (ab120331).
Exhibits greater fluorescence emission in neutral than acidic environments allowing optical measurement of synaptic vesicle content release.
Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags.- Canonical SMILES
- C1=C(C2=CC(=C(C=C2OC1=O)O)Cl)CCN.C(=O)(C(F)(F)F)O
- InChI
- InChI=1S/C11H10ClNO3.C2HF3O2/c12-8-4-7-6(1-2-13)3-11(15)16-10(7)5-9(8)14;3-2(4,5)1(6)7/h3-5,14H,1-2,13H2;(H,6,7)
- InChIKey
- OGHPXKAKVMJGQH-UHFFFAOYSA-N
- IUPAC name
- 4-(2-aminoethyl)-6-chloro-7-hydroxychromen-2-one;2,2,2-trifluoroacetic acid
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Chemical Structure - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)
2D chemical structure image of ab120866, FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate
Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)
All images provided by Thorsten LauFigure 1: Two neuronal cells stained with 50 μM FFN102 on differentiation day 10. Shown is a sum projection of a confocal z-stack. Accumulation of FFN102 can be observed along the neurites (arrows) and the cell soma (S).
Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)
Figure 2a: Images in the first row show a group of neuronal cells stained with 50 μM FFN102 (sum projection of a confocal stack). FFN102 localizes to structures on the cell soma (S) as well as neurites (arrows). Z indicates the area zoomed in for an additional z-stack.
Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)
Figure 2b: Zoomed in area of “Z” from figure 2b. The arrow indicates a globular structure on a neurite. S indicates FFN102 positive structures on the cell soma.
Cellular Activation - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)
Figure 3: FFN102 dye uptake inhibition on addition of VMAT2 inhibitor RO4-1284
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com