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AB120866

FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate

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(3 Publications)

MW 353.68 Da, Purity >98%. Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent DAT and VMAT2 substrate. Enables two-photon microscopic imaging of localization and activity of dopaminergic presynaptic terminals in the striatum of mouse acute brain slice. More selective for dopaminergic synapses than FFN511 (ab120331).
Exhibits greater fluorescence emission in neutral than acidic environments allowing optical measurement of synaptic vesicle content release.
Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags.
5 Images
Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (AB120866)
  • Fluorescent Cell Imaging

Unknown

Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (AB120866)

Figure 2a : Images in the first row show a group of neuronal cells stained with 50 μM FFN102 (sum projection of a confocal stack). FFN102 localizes to structures on the cell soma (S) as well as neurites (arrows). Z indicates the area zoomed in for an additional z-stack.

Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (AB120866)
  • Fluorescent Cell Imaging

Unknown

Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (AB120866)

Figure 2b : Zoomed in area of “Z” from figure 2b. The arrow indicates a globular structure on a neurite. S indicates FFN102 positive structures on the cell soma.

Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (AB120866)
  • Fluorescent Cell Imaging

Unknown

Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (AB120866)

All images provided by Thorsten Lau

Figure 1 : Two neuronal cells stained with 50 μM FFN102 on differentiation day 10. Shown is a sum projection of a confocal z-stack. Accumulation of FFN102 can be observed along the neurites (arrows) and the cell soma (S).

Chemical Structure - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (AB120866)
  • Chemical Structure

Lab

Chemical Structure - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (AB120866)

2D chemical structure image of ab120866, FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate

Cellular Activation - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (AB120866)
  • CellAct

Unknown

Cellular Activation - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (AB120866)

Figure 3 : FFN102 dye uptake inhibition on addition of VMAT2 inhibitor RO4-1284

Key facts

CAS number

1234064-11-9

Purity

>98%

Form

Solid

form

Molecular weight

353.68 Da

Molecular formula

C<sub>1</sub><sub>3</sub>H<sub>1</sub><sub>1</sub>ClF<sub>3</sub>NO<sub>5</sub>

PubChem

91885430

Nature

Synthetic

Solubility

Soluble in water to 50 mM

Biochemical name

4-(2-Aminoethyl)-6-chloro-7-hydroxychromen-2-one;2,2,2-trifluoroacetic acid

Biological description

Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent DAT and VMAT2 substrate. Enables two-photon microscopic imaging of localization and activity of dopaminergic presynaptic terminals in the striatum of mouse acute brain slice. More selective for dopaminergic synapses than FFN511 (ab120331).
Exhibits greater fluorescence emission in neutral than acidic environments allowing optical measurement of synaptic vesicle content release.
Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags.

Canonical smiles

C1=C(C2=CC(=C(C=C2OC1=O)O)Cl)CCN.C(=O)(C(F)(F)F)O

InChi

InChI=1S/C11H10ClNO3.C2HF3O2/c12-8-4-7-6(1-2-13)3-11(15)16-10(7)5-9(8)14;3-2(4,5)1(6)7/h3-5,14H,1-2,13H2;(H,6,7)

InChiKey

OGHPXKAKVMJGQH-UHFFFAOYSA-N

IUPAC Name

4-(2-aminoethyl)-6-chloro-7-hydroxychromen-2-one;2,2,2-trifluoroacetic acid

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 8:162 PubMed36424392

2022

Protein aggregation and calcium dysregulation are hallmarks of familial Parkinson's disease in midbrain dopaminergic neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Gurvir S Virdi,Minee L Choi,James R Evans,Zhi Yao,Dilan Athauda,Stephanie Strohbuecker,Raja S Nirujogi,Anna I Wernick,Noelia Pelegrina-Hidalgo,Craig Leighton,Rebecca S Saleeb,Olga Kopach,Haya Alrashidi,Daniela Melandri,Jimena Perez-Lloret,Plamena R Angelova,Sergiy Sylantyev,Simon Eaton,Simon Heales,Dmitri A Rusakov,Dario R Alessi,Tilo Kunath,Mathew H Horrocks,Andrey Y Abramov,Rickie Patani,Sonia Gandhi

Frontiers in cellular neuroscience 9:389 PubMed26500494

2015

Vesicular expression and release of ATP from dopaminergic neurons of the mouse retina and midbrain.

Applications

Unspecified application

Species

Unspecified reactive species

Tracy Ho,Andrew I Jobling,Ursula Greferath,Trinette Chuang,Archana Ramesh,Erica L Fletcher,Kirstan A Vessey

Proceedings of the National Academy of Sciences of the United States of America 110:870-5 PubMed23277566

2013

Fluorescent dopamine tracer resolves individual dopaminergic synapses and their activity in the brain.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela C Rodriguez,Daniela B Pereira,Anders Borgkvist,Minerva Y Wong,Candace Barnard,Mark S Sonders,Hui Zhang,Dalibor Sames,David Sulzer
View all publications
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