MW 353.68 Da, Purity >98%. Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent DAT and VMAT2 substrate. Enables two-photon microscopic imaging of localization and activity of dopaminergic presynaptic terminals in the striatum of mouse acute brain slice. More selective for dopaminergic synapses than FFN511 (ab120331).
Exhibits greater fluorescence emission in neutral than acidic environments allowing optical measurement of synaptic vesicle content release.
Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags.
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- NPJ Parkinson's disease 8:1622022Protein aggregation and calcium dysregulation are hallmarks of familial Parkinson's disease in midbrain dopaminergic neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGurvir S Virdi,Minee L Choi,James R Evans,Zhi Yao,Dilan Athauda,Stephanie Strohbuecker,Raja S Nirujogi,Anna I Wernick,Noelia Pelegrina-Hidalgo,Craig Leighton,Rebecca S Saleeb,Olga Kopach,Haya Alrashidi,Daniela Melandri,Jimena Perez-Lloret,Plamena R Angelova,Sergiy Sylantyev,Simon Eaton,Simon Heales,Dmitri A Rusakov,Dario R Alessi,Tilo Kunath,Mathew H Horrocks,Andrey Y Abramov,Rickie Patani,Sonia GandhiPubMed 36424392
- Frontiers in cellular neuroscience 9:3892015Vesicular expression and release of ATP from dopaminergic neurons of the mouse retina and midbrain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTracy Ho,Andrew I Jobling,Ursula Greferath,Trinette Chuang,Archana Ramesh,Erica L Fletcher,Kirstan A VesseyPubMed 26500494
- Proceedings of the National Academy of Sciences of the United States of America 110:870-52013Fluorescent dopamine tracer resolves individual dopaminergic synapses and their activity in the brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPamela C Rodriguez,Daniela B Pereira,Anders Borgkvist,Minerva Y Wong,Candace Barnard,Mark S Sonders,Hui Zhang,Dalibor Sames,David SulzerPubMed 23277566
Key facts
- CAS number
1234064-11-9
- Purity
> 98%
- Form
Solid
- Molecular weight
353.68 Da
- Molecular formula
C13H11ClF3NO5
- PubChem identifier
91885430
- Nature
Synthetic
Target data
Recommended products
MW 353.68 Da, Purity >98%. Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent DAT and VMAT2 substrate. Enables two-photon microscopic imaging of localization and activity of dopaminergic presynaptic terminals in the striatum of mouse acute brain slice. More selective for dopaminergic synapses than FFN511 (ab120331).
Exhibits greater fluorescence emission in neutral than acidic environments allowing optical measurement of synaptic vesicle content release.
Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags.
Key facts
- CAS number
1234064-11-9
- Purity
> 98%
- Form
Solid
- Molecular weight
353.68 Da
- Molecular formula
C13H11ClF3NO5
- PubChem identifier
91885430
- Nature
Synthetic
- Solubility
Soluble in water to 50 mM.
- Biochemical name
4-(2-Aminoethyl)-6-chloro-7-hydroxychromen-2-one;2,2,2-trifluoroacetic acid
- Biological description
Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent DAT and VMAT2 substrate. Enables two-photon microscopic imaging of localization and activity of dopaminergic presynaptic terminals in the striatum of mouse acute brain slice. More selective for dopaminergic synapses than FFN511 (ab120331).
Exhibits greater fluorescence emission in neutral than acidic environments allowing optical measurement of synaptic vesicle content release.
Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags.- Canonical SMILES
C1=C(C2=CC(=C(C=C2OC1=O)O)Cl)CCN.C(=O)(C(F)(F)F)O
- InChI
InChI=1S/C11H10ClNO3.C2HF3O2/c12-8-4-7-6(1-2-13)3-11(15)16-10(7)5-9(8)14;3-2(4,5)1(6)7/h3-5,14H,1-2,13H2;(H,6,7)
- InChIKey
OGHPXKAKVMJGQH-UHFFFAOYSA-N
- IUPAC name
4-(2-aminoethyl)-6-chloro-7-hydroxychromen-2-one;2,2,2-trifluoroacetic acid
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Store under desiccating conditions, The product can be stored for up to 12 months
Product promise
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Full details and terms and conditions can be found here:
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5 product images
Chemical Structure - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)
2D chemical structure image of ab120866, FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate
Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)
All images provided by Thorsten Lau
Figure 1: Two neuronal cells stained with 50 μM FFN102 on differentiation day 10. Shown is a sum projection of a confocal z-stack. Accumulation of FFN102 can be observed along the neurites (arrows) and the cell soma (S).
Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)
Figure 2a: Images in the first row show a group of neuronal cells stained with 50 μM FFN102 (sum projection of a confocal stack). FFN102 localizes to structures on the cell soma (S) as well as neurites (arrows). Z indicates the area zoomed in for an additional z-stack.
Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)
Figure 2b: Zoomed in area of “Z” from figure 2b. The arrow indicates a globular structure on a neurite. S indicates FFN102 positive structures on the cell soma.
Cellular Activation - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)
Figure 3: FFN102 dye uptake inhibition on addition of VMAT2 inhibitor RO4-1284
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