MW 804 Da, Purity >98%. Immunosuppressant. Complexes with FKBP-12 to inhibit calcineurin. Achieve your results faster with highly validated, pure and trusted compounds.
Images
Publications
Filter by
- The European respiratory journal 57:2020The mononuclear phagocyte system contributes to fibrosis in post-transplant obliterans bronchiolitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria-Pia Di Campli,Abdulkader Azouz,Assiya Assabban,Jessika Scaillet,Marion Splittgerber,Alexandra Van Keymeulen,Frederick Libert,Myriam Remmelink,Alain Le Moine,Philippe Lemaitre,Stanislas GorielyPubMed 33033147
- Autophagy 16:1683-16962019Oxidation of multiple MiT/TFE transcription factors links oxidative stress to transcriptional control of autophagy and lysosome biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongfeng Wang,Nana Wang,Delai Xu,Qilian Ma,Yang Chen,Shiqiang Xu,Qin Xia,Yan Zhang,Jochen H M Prehn,Guanghui Wang,Zheng YingPubMed 31826695
- Acta ophthalmologica 97:e688-e6992018Survival and functionality of xeno-free human embryonic stem cell-derived retinal pigment epithelial cells on polyester substrate after transplantation in rabbits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTanja Ilmarinen,Fabian Thieltges,Heidi Hongisto,Kati Juuti-Uusitalo,Arto Koistinen,Kai Kaarniranta,Ralf Brinken,Norbert Braun,Frank G Holz,Heli Skottman,Boris V StanzelPubMed 30593729
- Scientific reports 7:88882017Extracellular calcium modulates brown adipocyte differentiation and identity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInes Pramme-Steinwachs et. al.PubMed 28827782
- Molecular medicine reports 15:3001-30102017Protection of FK506 against neuronal apoptosis and axonal injury following experimental diffuse axonal injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing-Qin Huang,Jin-Ning Song,Feng-Wei Zheng,Hong-Gang Pang,Yong-Lin Zhao,Hua Gu,Jun-Jie ZhaoPubMed 28339015
- Journal of immunology (Baltimore, Md. : 1950) 197:3504-35112016Antibody Binding to CD4 Induces Rac GTPase Activation and Alters T Cell Migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesY Maurice Morillon et. al.PubMed 27694496
- The Journal of neuroscience : the official journal of the Society for Neuroscience 35:14756-702015An Endocytic Scaffolding Protein together with Synapsin Regulates Synaptic Vesicle Clustering in the Drosophila Neuromuscular Junction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesÅsa M E Winther,Olga Vorontsova,Kathryn A Rees,Tuomas Näreoja,Elena Sopova,Wei Jiao,Oleg ShupliakovPubMed 26538647
- Journal of neuroinflammation 12:422015Attenuation of microglial activation in a mouse model of Alzheimer's disease via NFAT inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLalida Rojanathammanee et. al.PubMed 25889879
- The Journal of biological chemistry 289:21727-372014Glucose activates TORC2-Gad8 protein via positive regulation of the cAMP/cAMP-dependent protein kinase A (PKA) pathway and negative regulation of the Pmk1 protein-mitogen-activated protein kinase pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdiel Cohen et. al.PubMed 24928510
- Neuroscience letters 510:82-72012Calcineurin is required for TRPV1-induced long-term depression of hippocampal interneurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTyron Jensen et. al.PubMed 22260796
Key facts
- CAS number
- 104987-11-3
- Purity
- > 98%
- Form
- Solid
- Source
- Streptomyces tsukubaensis
- Molecular weight
- 804 Da
- Molecular formula
- C44H69NO12
- PubChem identifier
- 445643
- Nature
- Native
Target data
Alternative names
12 kDa FK506-binding protein, 12 kDa FKBP, Calstabin 1, FK506 binding protein 1, FK506 binding protein 12, FK506 binding protein 1A (12kD), FK506 binding protein 1A 12kDa, FK506 binding protein T cell 12 kD, FK506-binding protein 1A, FKB1A_HUMAN, FKBP-1A, FKBP1, FKBP12 Exip3, FKBP12C, Immunophilin FKBP12, PKC12, PKCI2, PPIase, PPIase FKBP1A, Peptidyl-prolyl cis-trans isomerase FKBP1A, Protein kinase C inhibitor 2, Rotamase, peptidyl-prolyl cis-trans isomerase
Recommended products
MW 804 Da, Purity >98%. Immunosuppressant. Complexes with FKBP-12 to inhibit calcineurin. Achieve your results faster with highly validated, pure and trusted compounds.
Key facts
- CAS number
- 104987-11-3
- Purity
- > 98%
- Form
- Solid
- Source
- Streptomyces tsukubaensis
- Molecular weight
- 804 Da
- Molecular formula
- C44H69NO12
- PubChem identifier
- 445643
- Nature
- Native
- Solubility
Soluble in DMSO to 100 mM.
- Biochemical name
- Tacrolimus
- Biological description
Immunosuppressant. Complexes with FKBP-12 to inhibit calcineurin.
- Canonical SMILES
- CC1CC(C2C(CC(C(O2)(C(=O)C(=O)N3CCCCC3C(=O)OC(C(C(CC(=O)C(C=C(C1)C)CC=C)O)C)C(=CC4CCC(C(C4)OC)O)C)O)C)OC)OC
- Isomeric SMILES
- C[C@@H]1C[C@@H]([C@@H]2[C@H](C[C@H]([C@@](O2)(C(=O)C(=O)N3CCCC[C@H]3C(=O)O[C@@H]([C@@H]([C@H](CC(=O)[C@@H](/C=C(/C1)\C)CC=C)O)C)/C(=C/[C@@H]4CC[C@H]([C@@H](C4)OC)O)/C)O)C)OC)OC
- InChI
- InChI=1S/C44H69NO12/c1-10-13-31-19-25(2)18-26(3)20-37(54-8)40-38(55-9)22-28(5)44(52,57-40)41(49)42(50)45-17-12-11-14-32(45)43(51)56-39(29(6)34(47)24-35(31)48)27(4)21-30-15-16-33(46)36(23-30)53-7/h10,19,21,26,28-34,36-40,46-47,52H,1,11-18,20,22-24H2,2-9H3/b25-19+,27-21+/t26-,28+,29+,30-,31+,32-,33+,34-,36+,37-,38-,39+,40+,44+/m0/s1
- InChIKey
- QJJXYPPXXYFBGM-LFZNUXCKSA-N
- IUPAC name
- (1R,9S,12S,13R,14S,17R,18E,21S,23S,24R,25S,27R)-1,14-dihydroxy-12-[(E)-1-[(1R,3R,4R)-4-hydroxy-3-methoxycyclohexyl]prop-1-en-2-yl]-23,25-dimethoxy-13,19,21,27-tetramethyl-17-prop-2-enyl-11,28-dioxa-4-azatricyclo[22.3.1.04,9]octacos-18-ene-2,3,10,16-tetrone
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FKBP12 also known as FKBP-12 or FKBP12 protein is a small protein with a molecular weight of approximately 12 kDa. It belongs to the family of immunophilins and acts as a peptidyl-prolyl cis-trans isomerase (PPIase) facilitating the folding of proteins by catalyzing the isomerization of proline residues in polypeptides. The FKBP12 protein is expressed in various tissues throughout the body including the brain heart and skeletal muscles. It plays a mechanical role in binding certain macrolide antibiotics such as FK506 (tacrolimus) influencing their biological effects by altering protein conformation.
- Biological function summary
FKBP12 is significant in cellular mechanisms beyond protein folding. It interacts with and forms complexes with other proteins such as ryanodine receptors and transforming growth factor-beta (TGF-β) receptors impacting calcium signaling and cell growth. FKBP12's interaction with FK506 has been extensively studied because it forms a complex that inhibits calcineurin ultimately leading to immunosuppressive effects. This property of FKBP12 makes it important for modulating the immune response and has importance in transplant medicine.
- Pathways
FKBP12 is actively involved in calcium signaling and TGF-β signaling pathways. FKBP12 influences these pathways by binding to ryanodine receptors affecting calcium release from intracellular stores and also modulating the function of TGF-β receptors impacting cellular growth and proliferation processes. It is associated with proteins such as calcineurin and Smad proteins in these pathways maintaining cellular homeostasis and regulating immune and inflammatory responses.
- Associated diseases and disorders
FKBP12 plays a role in immunological and proliferative diseases. It is implicated in autoimmune disorders due to its interaction with FK506 leading to the suppression of unwanted immune responses. FKBP12 is also linked to cardiac hypertrophy as its modulation of calcium release through ryanodine receptors can affect heart muscle function. The protein interacts with calcineurin in these contexts contributing to pathological processes in these diseases. Researchers target FKBP12 with ELISA and other assays to investigate its involvement in these disorders and assess therapeutic interventions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Chemical Structure - FK506 (Tacrolimus), Immunosuppressant (ab120223)
2D chemical structure image of ab120223, FK506 (Tacrolimus), Immunosuppressant
Immunocytochemistry/ Immunofluorescence - FK506 (Tacrolimus), Immunosuppressant (ab120223)
Anti-IKB alpha antibody [E130] ab32518 staining IkBα/β in RAW 264.7 cells treated with FK506 (ab120223), by ICC/IF. Decrease in IkBα/β expression correlates with increased concentration of FK506, as described in literature.
The cells were incubated at 37°C for 3h in media containing different concentrations of ab120223 (FK506) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-IKB alpha antibody [E130] ab32518 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com