Fluo-2 AM, green fluorescent Ca2+ binding dye

Key facts

CAS number

1070771-36-6

Purity

>95%

Form

Solid

form

Molecular weight

1061 Da

Molecular formula

C₅₁H₅₂N₂O₂₃

PubChem

53390452

Nature

Synthetic

Solubility

Soluble in DMSO

Biochemical name

Fluo-2 AM

Biological description

High affinity, green fluorescent Ca²⁺ binding dye. Binds to intracellular Ca²⁺ (K_d = 230 nM). Cell-permeable. Fluorescence intensity increases upon Ca²⁺ binding. Ex₄₈₈/Em₅₁₅.

Canonical smiles

CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=CC(=O)C=C4OC5=C3C=CC(=C5)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

InChi

InChI=1S/C51H52N2O23/c1-30-7-13-41(52(21-47(60)72-26-67-32(3)55)22-48(61)73-27-68-33(4)56)45(17-30)64-15-16-65-46-18-36(8-14-42(46)53(23-49(62)74-28-69-34(5)57)24-50(63)75-29-70-35(6)58)51-39-11-9-37(59)19-43(39)76-44-20-38(10-12-40(44)51)71-25-66-31(2)54/h7-14,17-20H,15-16,21-29H2,1-6H3

IUPAC Name

acetyloxymethyl 2-[N-[2-(acetyloxymethoxy)-2-oxoethyl]-2-[2-[5-[3-(acetyloxymethoxy)-6-oxoxanthen-9-yl]-2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]-4-methylanilino]acetate

Excitation/Emission

Ex: 490nm, Em: 520nm