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MW 1061 Da, Purity >95%.

High affinity, green fluorescent Ca2+ binding dye. Binds to intracellular Ca2+ (Kd = 230 nM). Cell-permeable. Fluorescence intensity increases upon Ca2+ binding. Ex488/Em515.

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Images

Chemical Structure - Fluo-2 AM, green fluorescent Ca2+ binding dye (AB142775), expandable thumbnail

Publications

Key facts

CAS number
1070771-36-6
Purity
> 95%
Form
Solid
Molecular weight
1061 Da
Molecular formula
C51H52N2O23
PubChem identifier
53390452
Nature
Synthetic
Excitation/Emission
Ex: 490nm, Em: 520nm

Recommended products

MW 1061 Da, Purity >95%.

High affinity, green fluorescent Ca2+ binding dye. Binds to intracellular Ca2+ (Kd = 230 nM). Cell-permeable. Fluorescence intensity increases upon Ca2+ binding. Ex488/Em515.

Key facts

Purity
> 95%
PubChem identifier
53390452
Solubility

Soluble in DMSO.

Biochemical name
Fluo-2 AM
Biological description

High affinity, green fluorescent Ca2+ binding dye. Binds to intracellular Ca2+ (Kd = 230 nM). Cell-permeable. Fluorescence intensity increases upon Ca2+ binding. Ex488/Em515.

Canonical SMILES
CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=CC(=O)C=C4OC5=C3C=CC(=C5)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
InChI
InChI=1S/C51H52N2O23/c1-30-7-13-41(52(21-47(60)72-26-67-32(3)55)22-48(61)73-27-68-33(4)56)45(17-30)64-15-16-65-46-18-36(8-14-42(46)53(23-49(62)74-28-69-34(5)57)24-50(63)75-29-70-35(6)58)51-39-11-9-37(59)19-43(39)76-44-20-38(10-12-40(44)51)71-25-66-31(2)54/h7-14,17-20H,15-16,21-29H2,1-6H3
IUPAC name
acetyloxymethyl 2-[N-[2-(acetyloxymethoxy)-2-oxoethyl]-2-[2-[5-[3-(acetyloxymethoxy)-6-oxoxanthen-9-yl]-2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]-4-methylanilino]acetate
Excitation/Emission
Ex: 490nm, Em: 520nm

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions, The product can be stored for up to 12 months

Product promise

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1 product image

  • Chemical Structure - Fluo-2 AM, green fluorescent Ca2+ binding dye (ab142775), expandable thumbnail

    Chemical Structure - Fluo-2 AM, green fluorescent Ca2+ binding dye (ab142775)

    2D chemical structure image of ab142775, Fluo-2 AM, green fluorescent Ca2+ binding dye

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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