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AB241083

Fluo-5 AM, Fluorescent labeling reagent (Fluo-4 analogue)

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Fluo-5 AM (ab241083) is a cell permeable green fluorescent calcium-binding dye binds to intracellular calcium (Kd = ~2.3 µM). Analog of Fluo-4 and suitable for detecting intracellular calcium levels in the range of 1 µM to 1 mM that would saturate the response of Fluo-4.

- Excitation/Emission: Ex: 494 nm, Em: 516 nm
- Suitable for Flow cytometry and fluorescence microscopy
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Chemical Structure - Fluo-5 AM, Fluorescent labeling reagent (Fluo-4 analogue) (AB241083)
  • Chemical Structure

Supplier Data

Chemical Structure - Fluo-5 AM, Fluorescent labeling reagent (Fluo-4 analogue) (AB241083)

Chemical structure for Fluo-5F, AM *Cell permeant*

Key facts

Purity

>98%

Form

Solid

form

Molecular weight

740.6 Da

Molecular formula

C<sub>3</sub><sub>5</sub>H<sub>2</sub><sub>7</sub>F<sub>3</sub>N<sub>2</sub>O<sub>1</sub><sub>3</sub>

PubChem

24802868

Nature

Synthetic

Solubility

DMSO

Biochemical name

Fluo-5F

Biological description

Fluo-5 AM is an analogue of Fluo-4 with reduced calcium binding affinity (Kd = 2.3 μM). Suitable for detecting intracellular calcium levels in the range 1 μM to 1 mM. Cells can be loaded with Fluo-5 AM by adding the dissolved indicator directly to cells in culture dishes.

Fluo-5 AM has excitation and emission wavelengths at 494 and 516 nm respectively. Compatible with excitation by argon-ion lasers at 488 nm. Fluorescence inceases by >100-fold upon binding calcium.

Canonical smiles

C1=CC(=C(C=C1C2=C3C=C(C(=O)C=C3OC4=CC(=C(C=C42)F)O)F)OCCOC5=C(C=CC(=C5)F)N(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O

InChi

InChI=1S/C35H27F3N2O13/c36-18-2-4-24(40(15-33(47)48)16-34(49)50)30(8-18)52-6-5-51-29-7-17(1-3-23(29)39(13-31(43)44)14-32(45)46)35-19-9-21(37)25(41)11-27(19)53-28-12-26(42)22(38)10-20(28)35/h1-4,7-12,41H,5-6,13-16H2,(H,43,44)(H,45,46)(H,47,48)(H,49,50)

InChiKey

PITMCSLKSXHPOA-UHFFFAOYSA-N

IUPAC Name

2-[2-[2-[2-[bis(carboxymethyl)amino]-5-fluorophenoxy]ethoxy]-N-(carboxymethyl)-4-(2,7-difluoro-3-hydroxy-6-oxoxanthen-9-yl)anilino]acetic acid

Excitation/Emission

Ex: 494nm, Em: 516nm

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Biochemicals

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Chemical Structure": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store in the dark|Store under desiccating conditions
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Product protocols

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Product promise

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