Fluo-8 AM, a green fluorescent medium affinity calcium-binding dye binds to intracellular calcium (Kd = 390 nM). Fluorescence intensity increases upon Ca2+ binding.
- Cited in over 50 publications
- Cell-permeable
- Soluble in DMSO
- Brighter (1.5x) than Fluo-4 in cellular experiments
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- eLife 13:2024Interaction of human keratinocytes and nerve fiber terminals at the neuro-cutaneous unit.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristoph Erbacher,Sebastian Britz,Philine Dinkel,Thomas Klein,Markus Sauer,Christian Stigloher,Nurcan ÜçeylerPubMed 38225894
- Scientific reports 13:110452023Stressed neuronal cells can recover from profound membrane blebbing, nuclear condensation and mitochondrial fragmentation, but not from cytochrome c release.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenting You,Tao Zhou,Kèvin Knoops,Tos T J M Berendschot,Marc A M J van Zandvoort,Wilfred T V Germeraad,Birke Benedikter,Carroll A B Webers,Chris P M Reutelingsperger,Theo G M F GorgelsPubMed 37422517
- Journal of fungi (Basel, Switzerland) 9:2023MAP Kinase FgHog1 and Importin β FgNmd5 Regulate Calcium Homeostasis in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesLixin Zhang,Yiqing Li,Lanlan Dong,Kewei Sun,Hao Liu,Zhonghua Ma,Leiyan Yan,Yanni YinPubMed 37504696
- International journal of molecular sciences 24:2023Secretogranin III Selectively Promotes Vascular Leakage in the Deep Vascular Plexus of Diabetic Retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiyang Ji,Prabuddha Waduge,Yan Wu,Chengchi Huang,Avinash Kaur,Paola Oliveira,Hong Tian,Jinsong Zhang,J Timothy Stout,Christina Y Weng,Keith A Webster,Wei LiPubMed 37445707
- Science advances 9:eaaz94992023Bioengineered perfused human brain microvascular networks enhance neural progenitor cell survival, neurogenesis, and maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMax A Winkelman,Guohao DaiPubMed 37163593
- EMBO molecular medicine 15:e171012023Axin2 coupled excessive Wnt-glycolysis signaling mediates social defect in autism spectrum disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengmeng Wang,Panpan Xian,Weian Zheng,Zhenzhen Li,Andi Chen,Haoxiang Xiao,Chao Xu,Fei Wang,Honghui Mao,Han Meng,Youyi Zhao,Ceng Luo,Yazhou Wang,Shengxi WuPubMed 37078424
- Cells 12:2023Alpha1A- and Beta3-Adrenoceptors Interplay in Adipose Multipotent Mesenchymal Stromal Cells: A Novel Mechanism of Obesity-Driven Hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVadim Chechekhin,Anastasia Ivanova,Konstantin Kulebyakin,Veronika Sysoeva,Daria Naida,Mikhail Arbatsky,Nataliya Basalova,Maxim Karagyaur,Mariya Skryabina,Anastasia Efimenko,Olga Grigorieva,Natalia Kalinina,Vsevolod Tkachuk,Pyotr Tyurin-KuzminPubMed 36831252
- Cells 11:2022Dynamic Balance between PTH1R-Dependent Signal Cascades Determines Its Pro- or Anti-Osteogenic Effects on MSC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKonstantin Kulebyakin,Pyotr Tyurin-Kuzmin,Leila Sozaeva,Nikita Voloshin,Mikhail Nikolaev,Vadim Chechekhin,Maxim Vigovskiy,Veronika Sysoeva,Elizaveta Korchagina,Daria Naida,Maria VorontsovaPubMed 36359914
- Molecules (Basel, Switzerland) 27:2022Identification of a Partial and Selective TRPV1 Agonist CPIPC for Alleviation of Inflammatory Pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiying Dong,Qiqi Zhou,Qianqian Liang,Zhen Qiao,Yani Liu,Liming Shao,Kewei WangPubMed 36080196
- Antioxidants (Basel, Switzerland) 11:2022Muscone and (+)-Borneol Cooperatively Strengthen CREB Induction of Claudin 5 in IL-1-Induced Endothelium Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Chen Li,Yi Li,Yu-Ning Zhang,Qiong Zhao,Pei-Lin Zhang,Meng-Ru Sun,Bao-Lin Liu,Hua Yang,Ping LiPubMed 35892657
Key facts
- CAS number
- 1345980-40-6
- Form
- Solid
- Molecular weight
- 1046.94 g/mol
- Molecular formula
- C50H50N2O23
- Nature
- Synthetic
- Excitation/Emission
- Ex: 490nm, Em: 520nm
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Fluo-8 AM, a green fluorescent medium affinity calcium-binding dye binds to intracellular calcium (Kd = 390 nM). Fluorescence intensity increases upon Ca2+ binding.
- Cited in over 50 publications
- Cell-permeable
- Soluble in DMSO
- Brighter (1.5x) than Fluo-4 in cellular experiments
Key facts
- CAS number
- 1345980-40-6
- Form
- Solid
- Molecular weight
- 1046.94 g/mol
- Molecular formula
- C50H50N2O23
- Nature
- Synthetic
- Solubility
Soluble in DMSO.
- Biological description
Medium affinity green fluorescent calcium binding dye. Binds to intracellular calcium (Kd = 390 nM). Fluorescence intensity increases upon Ca2+ binding. Cell-permeable.
Fluo-8 (or Fluo-2 Medium Affinity) has been found to be brighter (1.5x) than Fluo-4 in cellular experiments. It offers improved cell loading and Ca2+ response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation at 490 nm and maximum emission at 520 nm. Fluo-8 loading can be performed at room temperature.- Canonical SMILES
- CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C5CCCCC5OCCOC1CC(CCC1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=2C4CCC(CC4OC3=CC(=O)C=CC=23)OCOC(C)=O
- InChI
- InChI=1S/C50H50N2O23/c1-30(53)65-25-70-37-12-14-39-44(20-37)75-43-19-36(58)11-13-38(43)50(39)35-10-15-41(52(23-48(61)73-28-68-33(4)56)24-49(62)74-29-69-34(5)57)45(18-35)64-17-16-63-42-9-7-6-8-40(42)51(21-46(59)71-26-66-31(2)54)22-47(60)72-27-67-32(3)55/h6-15,18-20H,16-17,21-29H2,1-5H3
- InChIKey
- BKYHTWFRZPRHNM-UHFFFAOYSA-N
- IUPAC name
- Bis(acetoxymethyl) 2,2'-((4-(6-(acetoxymethoxy)-3-oxo-3H-xanthen-9-yl)-2-(2-(bis(2-acetoxymethoxy)-2-oxoethyl)amino)phenoxy)ethoxy)phenyl)azanediyl)diacetate
- Excitation/Emission
- Ex: 490nm, Em: 520nm
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
Notes
Fluo-8® AM Working Solution: On the day of the experiment, either dissolve Fluo-8® AM in DMSO or thaw an aliquot of the indicator stock solution to room temperature. Prepare a dye working solution of 2 to 20 µM in a buffer of your choice (e.g., Hanks and Hepes buffer) with 0.04% Pluronic® F-127. For most cell lines, Fluo-8® AM at a final concentration of 4-5 μM is recommended. The exact concentration of indicators required for cell loading must be determined empirically.
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4 product images
Fluorescent Cell Imaging - Fluo-8 AM, green fluorescent calcium binding dye (ab142773)
U2OS cells were seeded overnight at 40,000 cells per 100 μL per well in a 96-well black wall/clear bottom costar plate. The growth medium was removed, and the cells were incubated with 100 μl of 4 μM Fluo-4 AM or Fluo-8® AM in HHBS at 37 °C, 5% CO2 incubator for 1 hour. The cells were washed twice with 200 μl HHBS, then imaged with a fluorescence microscope using FITC channel.
Chemical Structure - Fluo-8 AM, green fluorescent calcium binding dye (ab142773)
2D chemical structure image of ab142773, Fluo-8 AM, green fluorescent calcium binding dye
Cui et al PLoS One. 2013;8(3):e59921. doi: 10.1371/journal.pone.0059921. Epub 2013 Mar 20. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Fluo-8 AM, green fluorescent calcium binding dye (ab142773)
FFA treatments increase Ca2+ influx to induce ERS in β-TC3 cells.β-TC3 cells were incubated with FFA or BSA for 16 h, and then stimulated with 4 μM thapsigargin for 20 min to activate store-operated Ca2+ entry. Fluorescence densities of Ca2+ change were monitored in Fluo-8/AM-loaded β-TC3 cells after FFA or BSA treatments.
(From Figure 2B of Cui et al)
Functional Studies - Fluo-8 AM, green fluorescent calcium binding dye (ab142773)
Fluo-8 vs Fluo-4 sensitivity to calcium release in HEK-293 cells induced by Carbachol.
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