Fluo-8 AM, green fluorescent calcium binding dye

• Fluorescent Cell Imaging

Supplier Data

Fluorescent Cell Imaging - Fluo-8 AM, green fluorescent calcium binding dye (AB142773)

U2OS cells were seeded overnight at 40,000 cells per 100 μL per well in a 96-well black wall/clear bottom costar plate. The growth medium was removed, and the cells were incubated with 100 μl of 4 μM Fluo-4 AM or Fluo-8® AM in HHBS at 37 °C, 5% CO₂ incubator for 1 hour. The cells were washed twice with 200 μl HHBS, then imaged with a fluorescence microscope using FITC channel.

• FuncS

Supplier Data

Functional Studies - Fluo-8 AM, green fluorescent calcium binding dye (AB142773)

Fluo-8 vs Fluo-4 sensitivity to calcium release in HEK-293 cells induced by Carbachol.

• FuncS

PubMed

Functional Studies - Fluo-8 AM, green fluorescent calcium binding dye (AB142773)

FFA treatments increase Ca²⁺ influx to induce ERS in β-TC3 cells.

β-TC3 cells were incubated with FFA or BSA for 16 h, and then stimulated with 4 μM thapsigargin for 20 min to activate store-operated Ca²⁺ entry. Fluorescence densities of Ca²⁺ change were monitored in Fluo-8/AM-loaded β-TC3 cells after FFA or BSA treatments.

(From Figure 2B of Cui et al)

Cui et al PLoS One. 2013;8(3):e59921. doi: 10.1371/journal.pone.0059921. Epub 2013 Mar 20. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• Chemical Structure

Lab

Chemical Structure - Fluo-8 AM, green fluorescent calcium binding dye (AB142773)

2D chemical structure image of ab142773, Fluo-8 AM, green fluorescent calcium binding dye