Fluorescein di-β-D-galactopyranoside (FDG)

Key facts

CAS number

17817-20-8

Purity

>95%

Form

Solid

form

Molecular weight

656.6 Da

Molecular formula

C₃₂H₃₂O₁₅

PubChem

122063

Nature

Synthetic

Solubility

Soluble in DMSO

Soluble in ethanol

Soluble in water

Biochemical name

Fluorescein-digalactoside

Biological description

Ultra-sensitive substrate for *in vivo* and *in vitro* lacZ β-galactosidase detection. Widely used for detection of *E. coli lacZ* β-galactosidase by flow cytometry in both mammalian cells and gram-negative bacteria. Enters viable cells. Fluorescence signal proportional to lacZ enzymatic activity.

Canonical smiles

C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)OC5C(C(C(C(O5)CO)O)O)O)OC6=C3C=CC(=C6)OC7C(C(C(C(O7)CO)O)O)O

Isomeric smiles

C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)O[C@H]5[C@@H]([C@H]([C@H]([C@H](O5)CO)O)O)O)OC6=C3C=CC(=C6)O[C@H]7[C@@H]([C@H]([C@H]([C@H](O7)CO)O)O)O

InChi

InChI=1S/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(41)47-32/h1-10,21-28,30-31,33-40H,11-12H2/t21-,22-,23+,24+,25+,26+,27-,28-,30-,31-/m1/s1

InChiKey

ZTOBILYWTYHOJB-WBCGDKOGSA-N

IUPAC Name

3',6'-bis[[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]spiro[2-benzofuran-3,9'-xanthene]-1-one

Excitation/Emission

Ex: 488nm, Em: 512nm