Forskolin, Adenylyl cyclase activator

• Chemical Structure

Lab

Chemical Structure - Forskolin, Adenylyl cyclase activator (AB120058)

2D chemical structure image of ab120058, Forskolin, Adenylyl cyclase activator

• FuncS

Unknown

Functional Studies - Forskolin, Adenylyl cyclase activator (AB120058)

ab2541 staining XIAP in MCF7 cells treated with forskolin (ab120058), by ICC/IF. Increase in XIAP expression correlates with increased concentration of forskolin, as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120058 (forskolin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2541 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight^® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

• FuncS

PubMed

Functional Studies - Forskolin, Adenylyl cyclase activator (AB120058)

Effect of AC activation onto adult Mecp2X/Y and Mecp2308/Y Cortico-LA synapses.

A : A 10 minute application of the AC activator Forskolin (FSK, 50 μM) potentiates the Cortico-LA EPSC in young WT animals.

B : The same protocol is inefficient in adult WT mice (B1) and Mecp2308/Y mice (B2).

C : Summary plot of similar pharmacological experiments in WT and KO mice.

*** : P<0.001.

Number of recorded cells is indicated.

Gambino et al PLoS One. 2010 Jul 2;5(7):e11399. doi: 10.1371/journal.pone.0011399. Fig S4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• FuncS

Unknown

Functional Studies - Forskolin, Adenylyl cyclase activator (AB120058)

1e7 3T3-L1 cells were treated for 15 minutes with 1 µM dexamethasone (ab120743) and 1 µg x mL-1 insulin (ab123768) with the addition of 50 µM forskolin (ab120058), 0.5 mM IBMX (ab120840) or 0.1 mM 8-CPT-cAMP (ab120424), extracted according to the protocol. Lysates were diluted 50-fold, and tested in duplicates (+/-SD).