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AB120873

Fura-2 AM, Ca2+ selective fluorescent indicator

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(31 Publications)

Fura-2 AM (ab120873) is a high affinity Ca2+ selective, cell-permeable fluorescent indicator (Kd = 145 nM). Rapidly metabolized by cytoplasmic esterases leading to the active dye Fura 2. MW 1001.8.

- Excitation spectrum dependent on [Ca2+]i. Ex:340/380 nm. Em:505 nm
- Cited in over 15 publications
3 Images
Chemical Structure - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)
  • Chemical Structure

Lab

Chemical Structure - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)

2D chemical structure image of ab120873, Fura-2 AM, Ca2+ selective fluorescent indicator

Fluorescent Cell Imaging - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)
  • Fluorescent Cell Imaging

Lab

Fluorescent Cell Imaging - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)

Direct comparison of Abcam and competitor's Fura-2 AM. RAW 264.7 cells were loaded with 2 μM of Fura-2 AM from Abcam (ab120873) and competitor's in a DMSO/ HBSS solution for 30 minutes at room temperature and in the dark. After performing two washes with pre-warmed HBSS the cells were further incubated for 30 minutes at 37°C in the dark. Finally, washes with pre-warmed HBSS were performed and the live cells were imaged on a confocal microscope (using the same exposure settings) : excitation laser = 405nm, emission gate center = 519nm.

Fluorescent Cell Imaging - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)
  • Fluorescent Cell Imaging

Lab

Fluorescent Cell Imaging - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)

Direct comparison of Abcam and competitor's Fura-2 AM. RAW 264.7 cells were loaded with different concentrations of Fura-2 AM from Abcam (ab120873) and competitor's in a DMSO/ HBSS solution for 30 minutes at room temperature and in the dark. After performing two washes with pre-warmed HBSS the cells were further incubated for 30 minutes at 37°C in the dark. Finally, washes with pre-warmed HBSS were performed and the live cells were imaged on a confocal microscope (using the same exposure settings) : excitation laser = 405nm, emission gate center = 519nm.

Key facts

CAS number

108964-32-5

Purity

>95%

Form

Solid

form

Molecular weight

1001.8 Da

Molecular formula

C<sub>4</sub><sub>4</sub>H<sub>4</sub><sub>7</sub>N<sub>3</sub>O<sub>2</sub><sub>4</sub>

PubChem

3364574

Nature

Synthetic

Solubility

Soluble in DMSO to 10 mM

Biochemical name

Fura 2-AM

Biological description

High affinity Ca2+ selective fluorescent indicator (Kd = 145 nM). Rapidly metabolized by cytoplasmic esterases leading to the active dye Fura 2. Cell-permeable. Excitation spectrum dependent on [Ca2+]i. Ex340/380/Em505.

Canonical smiles

CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

InChi

InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3

InChiKey

VPSRLGDRGCKUTK-UHFFFAOYSA-N

IUPAC Name

acetyloxymethyl 2-[6-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate

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Product details

1 unit = 50 µg

  • 1 mg - 1 x 1 mg vial
  • 20 units - 20 x 50 µg vials
  • 10 units - 10 x 50 µg vials
  • 50 µg - 1 x 50 µg vial

Related protocols:

Fura-2 AM imaging protocol

Related Products:

Calcium indicators and ionophores

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular and cellular cardiology plus 12:100301 PubMed40485773

2025

PP2A-B56α is a key determinant of cardiac protein phosphorylation and functional responses to β-adrenergic signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Alican Güran,Yanlong Ji,Pan Fang,Kuan-Ting Pan,Henning Urlaub,Metin Avkiran,Christof Lenz,Kate L Weeks

International journal of molecular sciences 26: PubMed40244142

2025

MnO Nanocrystal-Induced Eryptosis Features Ca Overload, ROS and RNS Accumulation, Calpain Activation, Recruitment of Caspases, and Changes in the Lipid Order of Cell Membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuriy Kot,Volodymyr Prokopiuk,Vladimir Klochkov,Liliya Tryfonyuk,Pavel Maksimchuk,Andrey Aslanov,Kateryna Kot,Oleg Avrunin,Lesya Demchenko,Saulesh Kurmangaliyeva,Anatolii Onishchenko,Svetlana Yefimova,Ondrej Havranek,Anton Tkachenko

International journal of molecular sciences 26: PubMed40003936

2025

Niemann-Pick C-like Endolysosomal Dysfunction in DHDDS Patient Cells, a Congenital Disorder of Glycosylation, Can Be Treated with Miglustat.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah L Best,Sophie R Cook,Helen Waller-Evans,Emyr Lloyd-Evans

International journal of molecular sciences 25: PubMed39519237

2024

GdVO:Eu and LaVO:Eu Nanoparticles Exacerbate Oxidative Stress in L929 Cells: Potential Implications for Cancer Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuriy Kot,Vladimir Klochkov,Volodymyr Prokopiuk,Olha Sedyh,Liliya Tryfonyuk,Ganna Grygorova,Nina Karpenko,Oleksandr Tomchuk,Kateryna Kot,Anatolii Onishchenko,Svetlana Yefimova,Anton Tkachenko

Cell communication and signaling : CCS 22:533 PubMed39506805

2024

Breast cancer cells utilize T3 to trigger proliferation through cellular Ca modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ines Tawfik,Katharina Schlick,Julian Ostaku,Doruntina Bresilla,Sonja Gabrijelčič,Benjamin Gottschalk,Alwin Sokolowski,Ernst Malle,Katarina Kalinova,Martin Hirtl,Corina T Madreiter-Sokolowski

International journal of molecular sciences 25: PubMed39273556

2024

Left Ventricular Systolic Dysfunction in NBCe1-B/C-Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Clayton T Brady,Aniko Marshall,Lisa A Eagler,Thomas M Pon,Michael E Duffey,Brian R Weil,Jennifer K Lang,Mark D Parker

Scientific reports 14:10877 PubMed38740862

2024

Astroglial connexin 43 is a novel therapeutic target for chronic multiple sclerosis model.

Applications

Unspecified application

Species

Unspecified reactive species

Ezgi Ozdemir Takase,Ryo Yamasaki,Satoshi Nagata,Mitsuru Watanabe,Katsuhisa Masaki,Hiroo Yamaguchi,Jun-Ichi Kira,Hideyuki Takeuchi,Noriko Isobe

Translational neuroscience 15:20220341 PubMed38736723

2024

Suanzaoren decoction exerts its antidepressant effect via the CaMK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Zhang,Jiyuan Guo,Ce Zhang,Wenhua Wang,Shuailin Du,Xusheng Tian

Nature neuroscience 27:822-835 PubMed38589584

2024

Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding.

Applications

Unspecified application

Species

Unspecified reactive species

Ezgi Hacisuleyman,Caryn R Hale,Natalie Noble,Ji-Dung Luo,John J Fak,Misa Saito,Jin Chen,Jonathan S Weissman,Robert B Darnell

Experimental physiology 109:711-728 PubMed38500268

2024

Time course changes in in vivo muscle mechanical function and Ca regulation of force following experimentally induced gradual ovarian failure in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Avery Hinks,Benjamin E Dalton,Parastoo Mashouri,Luke D Flewwelling,William Glen Pyle,Arthur J Cheng,Geoffrey A Power
View all publications
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