MW 1001.8 Da, Purity >95%. High affinity Ca2+ selective fluorescent indicator (Kd = 145 nM). Rapidly metabolized by cytoplasmic esterases leading to the active dye Fura 2. Cell-permeable. Excitation spectrum dependent on [Ca2+]i. Ex340/380/Em505.
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- iScience 26:1079902023Hypereosinophilia causes progressive cardiac pathologies in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicola Laura Diny,Megan Kay Wood,Taejoon Won,Monica Vladut Talor,Clarisse Lukban,Djahida Bedja,Nadan Wang,Hannah Kalinoski,Abdel Daoud,C Conover Talbot,Brian Leei Lin,Daniela ČihákováPubMed 37829205
- Cells 12:2023Human ApoE2 Endows Stronger Contractility in Rat Cardiomyocytes Enhancing Heart Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Wu,Fujie Zhao,Venkata N Sure,Abdulgafar Ibrahim,Changjiang Yu,Sean M Carr,Ping SongPubMed 36766690
- Experimental and therapeutic medicine 24:7502022PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengjiang Li,Yan Zhang,Yongkui Li,Shuxing Xing,Shunqiang Li,Jing Lyu,Zhaonan BanPubMed 36561964
- iScience 25:1055002022Reduction of breast cancer extravasation via vibration activated osteocyte regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Song,Chun-Yu Lin,Xueting Mei,Liyun Wang,Lidan YouPubMed 36419845
- Frontiers in immunology 13:9185512022Complement membrane attack complex is an immunometabolic regulator of NLRP3 activation and IL-18 secretion in human macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGisela Jimenez-Duran,Joseph Kozole,Rachel Peltier-Heap,Eleanor R Dickinson,Christopher R Kwiatkowski,Francesca Zappacosta,Roland S Annan,Nicholas W Galwey,Eva-Maria Nichols,Louise K Modis,Martha Triantafilou,Kathy Triantafilou,Lee M BootyPubMed 36248901
- The EMBO journal 41:e1092052022ECM dimensionality tunes actin tension to modulate endoplasmic reticulum function and spheroid phenotypes of mammary epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFuiBoon Kai,Guanqing Ou,Richard W Tourdot,Connor Stashko,Guido Gaietta,Mark F Swift,Niels Volkmann,Alexandra F Long,Yulong Han,Hector H Huang,Jason J Northey,Andrew M Leidal,Virgile Viasnoff,David M Bryant,Wei Guo,Arun P Wiita,Ming Guo,Sophie Dumont,Dorit Hanein,Ravi Radhakrishnan,Valerie M WeaverPubMed 35880301
- Nature communications 13:14062022Human rhinovirus promotes STING trafficking to replication organelles to promote viral replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartha Triantafilou,Joshi Ramanjulu,Lee M Booty,Gisela Jimenez-Duran,Hakan Keles,Ken Saunders,Neysa Nevins,Emma Koppe,Louise K Modis,G Scott Pesiridis,John Bertin,Kathy TriantafilouPubMed 35301296
- The Journal of cell biology 220:2021Tyrosine phosphorylation of S1PR1 leads to chaperone BiP-mediated import to the endoplasmic reticulum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMumtaz Anwar,Md Ruhul Amin,Vijay Avin Balaji Ragunathrao,Jacob Matsche,Andrei Karginov,Richard D Minshall,Gary C H Mo,Yulia Komarova,Dolly MehtaPubMed 34652421
- International journal of biological sciences 17:3622-36332021Pancreatic cancer-derived exosomal microRNA-19a induces β-cell dysfunction by targeting ADCY1 and EPAC2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjing Pang,Weiyan Yao,Xin Dai,Aisen Zhang,Lidan Hou,Lei Wang,Yu Wang,Xin Huang,Xiangjun Meng,Lei LiPubMed 34512170
- The EMBO journal 40:e1056032021PIP2 depletion and altered endocytosis caused by expression of Alzheimer's disease-protective variant PLCγ2 R522.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmily Maguire,Georgina E Menzies,Thomas Phillips,Michael Sasner,Harriet M Williams,Magdalena A Czubala,Neil Evans,Emma L Cope,Rebecca Sims,Gareth R Howell,Emyr Lloyd-Evans,Julie Williams,Nicholas D Allen,Philip R TaylorPubMed 34254352
Key facts
- CAS number
- 108964-32-5
- Purity
- > 95%
- Form
- Solid
- Molecular weight
- 1001.8 Da
- Molecular formula
- C44H47N3O24
- PubChem identifier
- 3364574
- Nature
- Synthetic
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MW 1001.8 Da, Purity >95%. High affinity Ca2+ selective fluorescent indicator (Kd = 145 nM). Rapidly metabolized by cytoplasmic esterases leading to the active dye Fura 2. Cell-permeable. Excitation spectrum dependent on [Ca2+]i. Ex340/380/Em505.
Key facts
- CAS number
- 108964-32-5
- Purity
- > 95%
- Form
- Solid
- Molecular weight
- 1001.8 Da
- Molecular formula
- C44H47N3O24
- PubChem identifier
- 3364574
- Nature
- Synthetic
- Solubility
Soluble in DMSO to 10 mM.
- Biochemical name
- Fura 2-AM
- Biological description
High affinity Ca2+ selective fluorescent indicator (Kd = 145 nM). Rapidly metabolized by cytoplasmic esterases leading to the active dye Fura 2. Cell-permeable. Excitation spectrum dependent on [Ca2+]i. Ex340/380/Em505.
- Canonical SMILES
- CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
- InChI
- InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3
- InChIKey
- VPSRLGDRGCKUTK-UHFFFAOYSA-N
- IUPAC name
- acetyloxymethyl 2-[6-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
Notes
1 unit = 50 μg
- 1 mg - 1 x 1 mg vial
- 20 units - 20 x 50 μg vials
- 10 units - 10 x 50 μg vials
- 50 μg - 1 x 50 μg vial
Related protocols:
Calcium imaging using Fura-2 AM in motor neurons
Measurement of free cytosolic calcium concentration in single CHO-K1 cells
Product promise
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Full details and terms and conditions can be found here:
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3 product images
Chemical Structure - Fura-2 AM, Ca2+ selective fluorescent indicator (ab120873)
2D chemical structure image of ab120873, Fura-2 AM, Ca2+ selective fluorescent indicator
Fluorescent Cell Imaging - Fura-2 AM, Ca2+ selective fluorescent indicator (ab120873)
Direct comparison of Abcam and competitor's Fura-2 AM. RAW 264.7 cells were loaded with 2 μM of Fura-2 AM from Abcam (ab120873) and competitor's in a DMSO/ HBSS solution for 30 minutes at room temperature and in the dark. After performing two washes with pre-warmed HBSS the cells were further incubated for 30 minutes at 37°C in the dark. Finally, washes with pre-warmed HBSS were performed and the live cells were imaged on a confocal microscope (using the same exposure settings): excitation laser = 405nm, emission gate center = 519nm.
Fluorescent Cell Imaging - Fura-2 AM, Ca2+ selective fluorescent indicator (ab120873)
Direct comparison of Abcam and competitor's Fura-2 AM. RAW 264.7 cells were loaded with different concentrations of Fura-2 AM from Abcam (ab120873) and competitor's in a DMSO/ HBSS solution for 30 minutes at room temperature and in the dark. After performing two washes with pre-warmed HBSS the cells were further incubated for 30 minutes at 37°C in the dark. Finally, washes with pre-warmed HBSS were performed and the live cells were imaged on a confocal microscope (using the same exposure settings): excitation laser = 405nm, emission gate center = 519nm.
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