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MW 832 Da, Purity >95%. Fluorescent Ca2+ binding dye. Binds to Ca2+ with high affinity (Kd = 225 nM in cytosol). Fluoresces green and fluorescence intensity increases upon Ca2+ binding. Cell membrane impermeable.


Excitation = 340/380 nm. Emission = 505 nm.

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Images

Chemical Structure - Fura-2 K+ salt, fluorescent Ca2+ binding dye (AB142777), expandable thumbnail

Publications

Key facts

CAS number
113694-64-7
Purity
> 95%
Form
Solid
Molecular weight
832 Da
Molecular formula
C29H22K5N3O14
PubChem identifier
6098363
Nature
Synthetic
Excitation/Emission
Ex: 340nm, Em: 505nm

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MW 832 Da, Purity >95%. Fluorescent Ca2+ binding dye. Binds to Ca2+ with high affinity (Kd = 225 nM in cytosol). Fluoresces green and fluorescence intensity increases upon Ca2+ binding. Cell membrane impermeable.


Excitation = 340/380 nm. Emission = 505 nm.

Key facts

Purity
> 95%
PubChem identifier
6098363
Solubility

Soluble in water.

Biochemical name
Fura 2 pentapotassium salt, >=80%
Biological description

Fluorescent Ca2+ binding dye. Binds to Ca2+ with high affinity (Kd = 225 nM in cytosol). Fluoresces green and fluorescence intensity increases upon Ca2+ binding. Cell membrane impermeable.


Excitation = 340/380 nm. Emission = 505 nm.

Canonical SMILES
CC1=CC(=C(C=C1)N(CC(=O)[O-])CC(=O)[O-])OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)[O-])N(CC(=O)[O-])CC(=O)[O-].[K+].[K+].[K+].[K+].[K+]
InChI
InChI=1S/C29H27N3O14.5K/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40;;;;;/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42);;;;;/q;5*+1/p-5
InChIKey
HQKWBBCTCLUGSX-UHFFFAOYSA-I
IUPAC name
pentapotassium;2-[6-[bis(carboxylatomethyl)amino]-5-[2-[2-[bis(carboxylatomethyl)amino]-5-methylphenoxy]ethoxy]-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate
Excitation/Emission
Ex: 340nm, Em: 505nm

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions, The product can be stored for up to 12 months

Product promise

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1 product image

  • Chemical Structure - Fura-2 K+ salt, fluorescent Ca2+ binding dye (ab142777), expandable thumbnail

    Chemical Structure - Fura-2 K+ salt, fluorescent Ca2+ binding dye (ab142777)

    2D chemical structure image of ab142777, Fura-2 K+ salt, fluorescent Ca2+binding dye

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Product protocols

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