Fura-2 K+ salt, fluorescent Ca2+ binding dye

Key facts

CAS number

113694-64-7

Purity

>95%

Form

Solid

form

Molecular weight

832 Da

Molecular formula

C₂₉H₂₂K₅N₃O₁₄

PubChem

6098363

Nature

Synthetic

Solubility

Soluble in water

Biochemical name

Fura 2 pentapotassium salt, >=80%

Biological description

Fluorescent Ca2+ binding dye. Binds to Ca2+ with high affinity (Kd = 225 nM in cytosol). Fluoresces green and fluorescence intensity increases upon Ca2+ binding. Cell membrane impermeable. Excitation = 340/380 nm. Emission = 505 nm.

Canonical smiles

CC1=CC(=C(C=C1)N(CC(=O)[O-])CC(=O)[O-])OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)[O-])N(CC(=O)[O-])CC(=O)[O-].[K+].[K+].[K+].[K+].[K+]

InChi

InChI=1S/C29H27N3O14.5K/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40;;;;;/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42);;;;;/q;5*+1/p-5

InChiKey

HQKWBBCTCLUGSX-UHFFFAOYSA-I

IUPAC Name

pentapotassium;2-[6-[bis(carboxylatomethyl)amino]-5-[2-[2-[bis(carboxylatomethyl)amino]-5-methylphenoxy]ethoxy]-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate

Excitation/Emission

Ex: 340nm, Em: 505nm