MW 429.61 g/mol, Purity >98%. Selective GLI1 and 2 inhibitor (IC50 = 5 μM). Blocks the hedgehog/smoothened signaling pathway. Anticancer activity in vitro and in vivo.
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- Bioscience reports 40:2020Hedgehog signaling activation required for glypican-6-mediated regulation of invasion, migration, and epithelial-mesenchymal transition of gastric cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Zeng,Ran Yan,Guanghua Yang,Sen Xiang,Fuli ZhaoPubMed 32478377
- Cell death & disease 10:5742019GLI1 activation by non-classical pathway integrin αβ/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Dong et. al.PubMed 31366904
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 34:533-5462018Inactivation of Nell-1 in Chondrocytes Significantly Impedes Appendicular Skeletogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuichuan Qi,Jong Kil Kim,Pin Ha,Xiaoyan Chen,Eric Chen,Yao Chen,Jiayi Li,Hsin Chuan Pan,Mengliu Yu,Yasamin Mohazeb,Sophia Azer,Lloyd Baik,Jin Hee Kwak,Kang Ting,Xinli Zhang,Min Hu,Chia SooPubMed 30352124
- International journal of oncology 50:1623-16332017The mechanism of epithelial-mesenchymal transition induced by TGF-β1 in neuroblastoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing-Bo Shao,Zhi-Mei Gao,Wen-Yan Huang,Zhi-Bao LuPubMed 28393230
- Oncotarget 7:86134-861472016Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling.Applications:BLReactive species:HumanChuan Zhang,Yong Wang,YiFei Feng,Yue Zhang,Bing Ji,Sen Wang,Ye Sun,Chunyan Zhu,Dongsheng Zhang,Yueming SunPubMed 27863385
- Oncology reports 31:707-122013Inhibition of the Hedgehog pathway induces autophagy in pancreatic ductal adenocarcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYonghua Xu,Yong An,Xuehao Wang,Wenzhang Zha,Xiangcheng LiPubMed 24297612
Key facts
- CAS number
- 500579-04-4
- Purity
- > 98%
- Molecular weight
- 429.61 g/mol
- Molecular formula
- C27H35N5
- Nature
- Synthetic
Recommended products
MW 429.61 g/mol, Purity >98%. Selective GLI1 and 2 inhibitor (IC50 = 5 μM). Blocks the hedgehog/smoothened signaling pathway. Anticancer activity in vitro and in vivo.
Key facts
- CAS number
- 500579-04-4
- Purity
- > 98%
- Molecular weight
- 429.61 g/mol
- Molecular formula
- C27H35N5
- Nature
- Synthetic
- Solubility
Soluble in DMSO to 50 mM.
Soluble in ethanol to 100 mM.
- Biological description
Selective GLI1 and 2 inhibitor (IC50 = 5 μM). Blocks the hedgehog/smoothened signaling pathway. Anticancer activity in vitro and in vivo.
- Canonical SMILES
- CN(C)C1=CC=CC=C1CN2CCCN(CC3=CC=CC=C3N(C)C)C2C4=CC=NC=C4
- IUPAC name
- 2,2'-[[Dihydro-2-(4-pyridinyl)-1,3(2H,4H)-pyrimidinediyl]bis(methylene)]bis(N,N-dimethylbenzenamine)
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
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2 product images
Chemical Structure - GANT 61, GLI1 and 2 inhibitor (ab120904)
2D chemical structure image of ab120904, GANT 61, GLI1 and 2 inhibitor
Functional Studies - GANT 61, GLI1 and 2 inhibitor (ab120904)
Anti-MDC1 antibody ab11169 staining MDC1 in HCT116 cells treated with GANT 61 (ab120904), by ICC/IF. Increase in MDC1 expression correlates with increased concentration of GANT 61, as described in literature.
The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120904 (GANT 61) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-MDC1 antibody ab11169 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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