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AB120904

GANT 61, GLI1 and 2 inhibitor

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(6 Publications)

MW 429.61 g/mol, Purity >98%. Selective GLI1 and 2 inhibitor (IC50 = 5 μM). Blocks the hedgehog/smoothened signaling pathway. Anticancer activity in vitro and in vivo.
2 Images
Functional Studies - GANT 61, GLI1 and 2 inhibitor (AB120904)
  • FuncS

Unknown

Functional Studies - GANT 61, GLI1 and 2 inhibitor (AB120904)

ab11169 staining MDC1 in HCT116 cells treated with GANT 61 (ab120904), by ICC/IF. Increase in MDC1 expression correlates with increased concentration of GANT 61, as described in literature.
The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120904 (GANT 61) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11169 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Chemical Structure - GANT 61, GLI1 and 2 inhibitor (AB120904)
  • Chemical Structure

Lab

Chemical Structure - GANT 61, GLI1 and 2 inhibitor (AB120904)

2D chemical structure image of ab120904, GANT 61, GLI1 and 2 inhibitor

Key facts

CAS number

500579-04-4

Purity

>98%

Molecular weight

429.61 g/mol

Molecular formula

C<sub>2</sub><sub>7</sub>H<sub>3</sub><sub>5</sub>N<sub>5</sub>

Nature

Synthetic

Solubility

Soluble in DMSO to 50 mM

Soluble in ethanol to 100 mM

Biological description

Selective GLI1 and 2 inhibitor (IC50 = 5 μM). Blocks the hedgehog/smoothened signaling pathway. Anticancer activity in vitro and in vivo.

Canonical smiles

CN(C)C1=CC=CC=C1CN2CCCN(CC3=CC=CC=C3N(C)C)C2C4=CC=NC=C4

IUPAC Name

2,2'-[[Dihydro-2-(4-pyridinyl)-1,3(2H,4H)-pyrimidinediyl]bis(methylene)]bis(N,N-dimethylbenzenamine)

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB97180

Goat Anti-Rat IgM mu chain (HRP)

  • 5
  • 1
Secondary Antibodies

AB125919

Rabbit monoclonal [H19-5] Anti-Human kappa light chain

RabMAb|Recombinant

Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

  • 0
  • 0
Secondary Antibodies

AB97136

Rabbit Anti-Chicken IgY H&L

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (AB97136)

  • 0
  • 0
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 40: PubMed32478377

2020

Hedgehog signaling activation required for glypican-6-mediated regulation of invasion, migration, and epithelial-mesenchymal transition of gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zeng,Ran Yan,Guanghua Yang,Sen Xiang,Fuli Zhao

Cell death & disease 10:574 PubMed31366904

2019

GLI1 activation by non-classical pathway integrin αβ/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Dong,Hongchang Liu,Wen Zhou,Fan Zhang,Chuan Li,Jun Chen,Chenjun Tan,Bo Tang,Peiwu Yu

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 34:533-546 PubMed30352124

2018

Inactivation of Nell-1 in Chondrocytes Significantly Impedes Appendicular Skeletogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Huichuan Qi,Jong Kil Kim,Pin Ha,Xiaoyan Chen,Eric Chen,Yao Chen,Jiayi Li,Hsin Chuan Pan,Mengliu Yu,Yasamin Mohazeb,Sophia Azer,Lloyd Baik,Jin Hee Kwak,Kang Ting,Xinli Zhang,Min Hu,Chia Soo

International journal of oncology 50:1623-1633 PubMed28393230

2017

The mechanism of epithelial-mesenchymal transition induced by TGF-β1 in neuroblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Bo Shao,Zhi-Mei Gao,Wen-Yan Huang,Zhi-Bao Lu

Oncotarget 7:86134-86147 PubMed27863385

2016

Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling.

Applications

BL

Species

Human

Chuan Zhang,Yong Wang,YiFei Feng,Yue Zhang,Bing Ji,Sen Wang,Ye Sun,Chunyan Zhu,Dongsheng Zhang,Yueming Sun

Oncology reports 31:707-12 PubMed24297612

2013

Inhibition of the Hedgehog pathway induces autophagy in pancreatic ductal adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yonghua Xu,Yong An,Xuehao Wang,Wenzhang Zha,Xiangcheng Li
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