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AB145459

Green CMFDA (5-Chloromethylfluorescein diacetate), Green fluorescent dye

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(4 Publications)

MW 464.8 Da, Purity >95%. Cell-permeable green fluorescent dye. Does not affect cell viability or proliferation. Transforms intracellularly into a cell-impermeant, fluorescent product. Displays fluorescence for at least 72 hours. Brightly fluorescent, non-toxic and stable at physiological pH. Allows for multigenerational tracking of cellular movements.

View Alternative Names

DFN7, Deafness, Deafness X-linked 7, FAEES3, Fatty Acid Ethyl Ester Synthase III, GST class-pi, GST3, GSTP, GSTP1-1, GSTP1_HUMAN, Glutathione S Transferase 3, Glutathione S Transferase Pi, Glutathione S-transferase P, Glutathione S-transferase pi 1, X linked 7

1 Images
Chemical Structure - Green CMFDA (5-Chloromethylfluorescein diacetate), Green fluorescent dye (AB145459)
  • Chemical Structure

Lab

Chemical Structure - Green CMFDA (5-Chloromethylfluorescein diacetate), Green fluorescent dye (AB145459)

2D chemical structure image of ab145459, Green CMFDA (5-Chloromethylfluorescein diacetate), Green fluorescent dye

Key facts

CAS number

136832-63-8

Purity

>95%

Molecular weight

464.8 Da

Molecular formula

C<sub>2</sub><sub>5</sub>H<sub>1</sub><sub>7</sub>ClO<sub>7</sub>

PubChem

122297

Nature

Synthetic

Solubility

Soluble in DMSO

Biochemical name

5-Chloromethylfluorescein diacetate

Biological description

Cell-permeable green fluorescent dye. Does not affect cell viability or proliferation. Transforms intracellularly into a cell-impermeant, fluorescent product. Displays fluorescence for at least 72 hours. Brightly fluorescent, non-toxic and stable at physiological pH. Allows for multigenerational tracking of cellular movements.

Canonical smiles

CC(=O)OC1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)CCl)C(=O)O3)C5=C(O2)C=C(C=C5)OC(=O)C

InChi

InChI=1S/C25H17ClO7/c1-13(27)30-16-4-7-20-22(10-16)32-23-11-17(31-14(2)28)5-8-21(23)25(20)19-6-3-15(12-26)9-18(19)24(29)33-25/h3-11H,12H2,1-2H3

InChiKey

IPJDHSYCSQAODE-UHFFFAOYSA-N

IUPAC Name

[6'-acetyloxy-5-(chloromethyl)-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl] acetate

Excitation/Emission

Ex: 492nm, Em: 516nm

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST3 also known as GST pi or GSTP is a protein part of the glutathione S-transferase family. These enzymes are involved in detoxifying processes by catalyzing the conjugation of glutathione to harmful substances. GSTP is approximately 23 kDa in size and is predominantly expressed in mammalian tissues including the liver and lung. These tissues play active roles in detoxifying external chemicals and managing oxidative stress in the body.
Biological function summary

GST3 contributes to the detoxification of xenobiotics and electrophilic compounds playing a protective role against cellular damage. It can exist as homo-dimers or form hetero-dimers with other GST family members participating in larger enzyme complexes. Through conjugation reactions GSTP helps in the detoxification processes thereby aiding cellular defenses and maintaining cellular redox balance.

Pathways

GST3 is an integral part of the phase II detoxification pathway and oxidative stress response. It interacts closely with other proteins such as Nrf2 (Nuclear factor erythroid 2-related factor 2) and helps regulate the transcription of antioxidant proteins that protect against oxidative damage. This enzyme plays a role in modulating the MAPK signaling pathway which influences cell proliferation and apoptosis.

GSTP has links to several cancer types including liver and lung cancer. Its overexpression in tumors suggests its role in cancer cell survival and resistance to chemotherapy. GSTP interacts with cancer-associated proteins like c-Jun N-terminal kinase (JNK) influencing pathways that lead to aberrant cellular growth and transformation. It also has associations with neurodegenerative diseases given its role in counteracting oxidative stress which commonly underpins neurodegeneration.
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Product protocols

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:18553 PubMed39122790

2024

The microbial metabolite p-cresol compromises the vascular barrier and induces endothelial cytotoxicity and inflammation in a 3D human vessel-on-a-chip.

Applications

Unspecified application

Species

Unspecified reactive species

Sakulrat Mankhong,Thittaya Den-Udom,Tanotnon Tanawattanasuntorn,Thunwarat Suriyun,Kenjiro Muta,Chagriya Kitiyakara,Pimonrat Ketsawatsomkron

Osteoarthritis and cartilage open 6:100499 PubMed39076683

2024

Chondroprotection of articular cartilage integrity: Utilizing ultrasonic scalpel and hyperosmolar irrigation solution during cutting.

Applications

Unspecified application

Species

Unspecified reactive species

Nisreen Mohammed Al-Namnam,Aneta T Luczak,Irene Yang,Xuan Li,Margaret Lucas,Andrew C Hall,A Hamish R W Simpson

International journal of molecular sciences 23: PubMed36499060

2022

Cigarette Smoke Extract Induces p38 MAPK-Initiated, Fas-Mediated Eryptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ignazio Restivo,Alessandro Attanzio,Ilenia Concetta Giardina,Francesca Di Gaudio,Luisa Tesoriere,Mario Allegra

International journal of molecular sciences 22: PubMed34829984

2021

Study of the Role of the Tyrosine Kinase Receptor MerTK in the Development of Kidney Ischemia-Reperfusion Injury in RCS Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Pelé,Sebastien Giraud,Sandrine Joffrion,Virginie Ameteau,Adriana Delwail,Jean-Michel Goujon,Laurent Macchi,Thierry Hauet,Fatima Dkhissi,Omar Benzakour
View all publications
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