MW 266.34 g/mol, Purity >98%. Potent scavenger of reactive oxygen species with antiangiogenic, antitumor and anti-inflammatory properties. Mechanisms of action include inhibition of ras signalling and activation of the mitochondrial transistion pore via cyclophilin D induction. Inhibits NF-κB signaling, upstream of VEGF. Blood-brain barrier permeable.
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Key facts
- CAS number
- 35354-74-6
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 266.34 g/mol
- Molecular formula
- C18H18O2
- Nature
- Synthetic
Recommended products
MW 266.34 g/mol, Purity >98%. Potent scavenger of reactive oxygen species with antiangiogenic, antitumor and anti-inflammatory properties. Mechanisms of action include inhibition of ras signalling and activation of the mitochondrial transistion pore via cyclophilin D induction. Inhibits NF-κB signaling, upstream of VEGF. Blood-brain barrier permeable.
Key facts
- CAS number
- 35354-74-6
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 266.34 g/mol
- Molecular formula
- C18H18O2
- Nature
- Synthetic
- Solubility
Soluble in DMSO to 100 mM.
Soluble in ethanol to 100 mM.
- Biological description
Potent scavenger of reactive oxygen species with antiangiogenic, antitumor and anti-inflammatory properties. Mechanisms of action include inhibition of ras signalling and activation of the mitochondrial transistion pore via cyclophilin D induction. Inhibits NF-κB signaling, upstream of VEGF. Blood-brain barrier permeable.
- IUPAC name
- 5,3'-Diallyl-2,4'-dihydroxybiphenyl
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
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3 product images
Chemical Structure - Honokiol, anti-inflammatory and chemotherapeutic agent (ab120647)
2D chemical structure image of ab120647, Honokiol, anti-inflammatory and chemotherapeutic agent
Functional Studies - Honokiol, anti-inflammatory and chemotherapeutic agent (ab120647)
MCF7 cells were incubated at 37°C for 6h with vehicle control (0 μM) and different concentrations of honokiol(ab120647). Increased expression of acetyl coenzyme A carboxylase (phospho S79)(Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody ab31931) in MCF7 cells correlates with an increase in honokiol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody ab31931 at 1 μg/ml and Anti-beta Actin antibody ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution and visualised using ECL development solution.
Functional Studies - Honokiol, anti-inflammatory and chemotherapeutic agent (ab120647)
MCF7 cells were incubated at 37°C for 6h with vehicle control (0 μM) and different concentrations of honokiol(ab120647). Increased expression of AMPKα 1 + AMPKα 2 (phospho T172) (Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody ab23875) in MCF7 cells correlates with an increase in honokiol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody ab23875 at 1/1000 dilution and Anti-beta Actin antibody ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution and visualised using ECL development solution.
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