MW 196.64 g/mol, Purity >99%. Inhibits DNA methylation by inhibition of MAPK (EC50 = 2.96 μM). Multiple, direct effects on vascular smooth muscle; hyperpolarization, inhibition of IP3-induced release of Ca2+ and formation of NO leading to cGMP-mediated vasodilation. DNMT inhibitor (IC50 = 2 μM).
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Key facts
- CAS number
- 304-20-1
- Purity
- > 99%
- Form
- Solid
- Molecular weight
- 196.64 g/mol
- Molecular formula
- C8H8N4.HCl
- Nature
- Synthetic
Recommended products
MW 196.64 g/mol, Purity >99%. Inhibits DNA methylation by inhibition of MAPK (EC50 = 2.96 μM). Multiple, direct effects on vascular smooth muscle; hyperpolarization, inhibition of IP3-induced release of Ca2+ and formation of NO leading to cGMP-mediated vasodilation. DNMT inhibitor (IC50 = 2 μM).
Key facts
- CAS number
- 304-20-1
- Purity
- > 99%
- Form
- Solid
- Molecular weight
- 196.64 g/mol
- Molecular formula
- C8H8N4.HCl
- Nature
- Synthetic
- Solubility
Soluble in water to 100 mM.
- Biological description
Inhibits DNA methylation by inhibition of MAPK (EC50 = 2.96 μM). Multiple, direct effects on vascular smooth muscle; hyperpolarization, inhibition of IP3-induced release of Ca2+ and formation of NO leading to cGMP-mediated vasodilation. DNMT inhibitor (IC50 = 2 μM).
- IUPAC name
- 1-Hydrazinylpthalazine hydrochloride
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
- Storage information
- The product can be stored for up to 12 months
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2 product images
Chemical Structure - Hydralazine hydrochloride, DNA methylation inhibitor. (ab120863)
2D chemical structure image of ab120863, Hydralazine hydrochloride, DNA methylation inhibitor.
Functional Studies - Hydralazine hydrochloride, DNA methylation inhibitor. (ab120863)
Jurkat cells were incubated at 37°C for 5 days with vehicle control (0 μM) and different concentrations of hydralazine hydrochloride (ab120863). Decreased expression of DNMT1 (Anti-Dnmt1 antibody ab19905) in Jurkat cells correlates with an increase in hydralazine hydrochloride concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with Anti-Dnmt1 antibody ab19905 at 1 μg/ml and Anti-beta Actin antibody - Loading Control ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution and visualised using ECL development solution.
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