Hydralazine hydrochloride, DNA methylation inhibitor.

• FuncS

Unknown

Functional Studies - Hydralazine hydrochloride, DNA methylation inhibitor. (AB120863)

Jurkat cells were incubated at 37°C for 5 days with vehicle control (0 μM) and different concentrations of hydralazine hydrochloride (ab120863). Decreased expression of DNMT1 (ab19905) in Jurkat cells correlates with an increase in hydralazine hydrochloride concentration as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate) 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab19905 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

• Chemical Structure

Lab

Chemical Structure - Hydralazine hydrochloride, DNA methylation inhibitor. (AB120863)

2D chemical structure image of ab120863, Hydralazine hydrochloride, DNA methylation inhibitor.