Indo-1 K+ salt, fluorescent Ca2+ indicator

Key facts

CAS number

132319-56-3

Purity

>95%

Form

Solid

form

Molecular weight

840.1 Da

Molecular formula

C₃₂H₂₆K₅N₃O₁₂

PubChem

6098030

Nature

Synthetic

Solubility

Soluble in water

Biochemical name

Indo 1 pentapotassium salt

Biological description

Cell-impermeable, fluorescent, ratiometric Ca2+ indicator. Binds to Ca2+ with high affinity (Kd = 230 nM). Fluoresces blue/violet and fluorescence increases upon Ca2+ binding. Cell-permeable form, Indo 1 AM (ab142778) also available. Emission and excitation shifts from 475 to 401 nm and 346 to 330 nm respectively depending on Ca2+ binding.

Canonical smiles

CC1=CC(=C(C=C1)N(CC(=O)[O-])CC(=O)[O-])OCCOC2=C(C=CC(=C2)C3=CC4=C(N3)C=C(C=C4)C(=O)[O-])N(CC(=O)[O-])CC(=O)[O-].[K+].[K+].[K+].[K+].[K+]

InChi

InChI=1S/C32H31N3O12.5K/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22;;;;;/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45);;;;;/q;5*+1/p-5

InChiKey

VGZPRMSXVNDMAN-UHFFFAOYSA-I

IUPAC Name

pentapotassium;2-[4-[bis(carboxylatomethyl)amino]-3-[2-[2-[bis(carboxylatomethyl)amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylate