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MW 747.1 Da, Purity >99%. Ca2+ ionophore. Useful when calcium-dose response data are not required. Ion specificity Mn2+>Ca2+>Mg2+>Sr2+>Ba2+.

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Key facts

CAS number

56092-82-1

Purity

> 99%

Form

Solid

Source

Streptomyces conglobatus

Molecular weight

747.1 Da

Molecular formula

C41H70CaO9

PubChem identifier

6446270

Nature

Native

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MW 747.1 Da, Purity >99%. Ca2+ ionophore. Useful when calcium-dose response data are not required. Ion specificity Mn2+>Ca2+>Mg2+>Sr2+>Ba2+.

Key facts

Purity

> 99%

Source

Streptomyces conglobatus

PubChem identifier

6446270

Biochemical name

Ionomycin calcium

Biological description

Ca2+ ionophore. Useful when calcium-dose response data are not required. Ion specificity Mn2+>Ca2+>Mg2+>Sr2+>Ba2+.

Canonical SMILES

CC(CCC(=O)[O-])CC(C)CC(C)C(=O)C=C(C(C)CC(C)CC=CC(C)C(C(C)C(CC1CCC(O1)(C)C2CCC(O2)(C)C(C)O)O)O)[O-].[Ca+2]

Isomeric SMILES

C[C@H](CCC(=O)[O-])C[C@H](C)C[C@H](C)C(=O)/C=C(/[C@H](C)C[C@H](C)C/C=C/[C@@H](C)[C@H]([C@@H](C)[C@H](C[C@@H]1CC[C@@](O1)(C)[C@H]2CC[C@@](O2)(C)[C@@H](C)O)O)O)\[O-].[Ca+2]

InChI

InChI=1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1

InChIKey

WKRWUYKLUMMAKG-WYGBAUISSA-L

IUPAC name

calcium;(4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-19,21-dihydroxy-22-[(2S,5S)-5-[(2R,5S)-5-[(1R)-1-hydroxyethyl]-5-methyloxolan-2-yl]-5-methyloxolan-2-yl]-4,6,8,12,14,18,20-heptamethyl-11-oxido-9-oxodocosa-10,16-dienoate

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

+4°C

Storage information

Store under desiccating conditions, The product can be stored for up to 12 months

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Chemical Structure - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Chemical Structure - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    2D chemical structure image of ab120116, Ionomycin Ca2+ Salt, Ca2+ ionophore

  • Immunoprecipitation - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Immunoprecipitation - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    Histone H3 (citrulline R8) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate with ab219406 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab219406 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate 10 μg (Input).
    Lane 2: ab219406 IP in HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219406 in HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (AB219406)

    Predicted band size: 15 kDa

    Exposure time: 1s

  • Flow Cytometry (Intracellular) - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Flow Cytometry (Intracellular) - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) transfected with empty vector (left panel) or PADI4 (WT, right panel), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, labeling Histone H3 (citrulline R8) with ab219406 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 647) at 1/2000 dilution.

    Positive signal is obtained from HEK-293T cells transfected with WT PADI4 treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours.

  • Immunoprecipitation - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Immunoprecipitation - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    Histone H3 (citrulline R17) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate with ab219407 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab219407 at 1/1000 dilution.

    VeriBlot for IP secondary antibody (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate 10 μg (Input).
    Lane 2: ab219407 IP in HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219407 in HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (AB219407)

    Predicted band size: 15 kDa

    Exposure time: 1s

  • Flow Cytometry (Intracellular) - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Flow Cytometry (Intracellular) - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) transfected with empty vector (left panel) or PADI4 (WT, right panel), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, labeling Histone H3 (citrulline R17) with ab219407 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 647) at 1/2000 dilution.

    Positive signal is obtained from HEK-293T cells transfected with WT PADI4 treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours.

  • Functional Studies - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Functional Studies - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    ab58668 staining ATF3 in A549 cells treated with ionomycin Ca2+ salt (ab120116), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin Ca2+ salt, as described in literature.
    The cells were incubated at 37°C for 2h in media containing different concentrations of ab120116 (ionomycin Ca2+ salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • Sandwich ELISA - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Sandwich ELISA - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    Sandwich ELISA - IFN gamma Human ELISA Kit (ab46025)

    Jurkat were stimulated for 48 hours with 50 ng x mL-1 of PMA (ab120297) and 1 uM Ionomycin (ab120116) and PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Cell free supernatants were tested, showing results after background signal was subtracted (duplicates +/- SD).

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