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MW 747.1 Da, Purity >99%. Ca2+ ionophore. Useful when calcium-dose response data are not required. Ion specificity Mn2+>Ca2+>Mg2+>Sr2+>Ba2+.

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Images

Chemical Structure - Ionomycin Ca2+ Salt, Ca2+ ionophore (AB120116), expandable thumbnail
  • Immunoprecipitation - Ionomycin Ca2+ Salt, Ca2+ ionophore (AB120116), expandable thumbnail
  • Flow Cytometry (Intracellular) - Ionomycin Ca2+ Salt, Ca2+ ionophore (AB120116), expandable thumbnail
  • Immunoprecipitation - Ionomycin Ca2+ Salt, Ca2+ ionophore (AB120116), expandable thumbnail
  • Flow Cytometry (Intracellular) - Ionomycin Ca2+ Salt, Ca2+ ionophore (AB120116), expandable thumbnail

Publications

Key facts

CAS number
56092-82-1
Purity
> 99%
Form
Solid
Source
Streptomyces conglobatus
Molecular weight
747.1 Da
Molecular formula
C41H70CaO9
PubChem identifier
6446270
Nature
Native

Target data

Recommended products

MW 747.1 Da, Purity >99%. Ca2+ ionophore. Useful when calcium-dose response data are not required. Ion specificity Mn2+>Ca2+>Mg2+>Sr2+>Ba2+.

Key facts

Purity
> 99%
Source
Streptomyces conglobatus
PubChem identifier
6446270
Biochemical name
Ionomycin calcium
Biological description

Ca2+ ionophore. Useful when calcium-dose response data are not required. Ion specificity Mn2+>Ca2+>Mg2+>Sr2+>Ba2+.

Canonical SMILES
CC(CCC(=O)[O-])CC(C)CC(C)C(=O)C=C(C(C)CC(C)CC=CC(C)C(C(C)C(CC1CCC(O1)(C)C2CCC(O2)(C)C(C)O)O)O)[O-].[Ca+2]
Isomeric SMILES
C[C@H](CCC(=O)[O-])C[C@H](C)C[C@H](C)C(=O)/C=C(/[C@H](C)C[C@H](C)C/C=C/[C@@H](C)[C@H]([C@@H](C)[C@H](C[C@@H]1CC[C@@](O1)(C)[C@H]2CC[C@@](O2)(C)[C@@H](C)O)O)O)\[O-].[Ca+2]
InChI
InChI=1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1
InChIKey
WKRWUYKLUMMAKG-WYGBAUISSA-L
IUPAC name
calcium;(4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-19,21-dihydroxy-22-[(2S,5S)-5-[(2R,5S)-5-[(1R)-1-hydroxyethyl]-5-methyloxolan-2-yl]-5-methyloxolan-2-yl]-4,6,8,12,14,18,20-heptamethyl-11-oxido-9-oxodocosa-10,16-dienoate

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store under desiccating conditions, The product can be stored for up to 12 months

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8 product images

  • Chemical Structure - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Chemical Structure - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    2D chemical structure image of ab120116, Ionomycin Ca2+ Salt, Ca2+ ionophore

  • Immunoprecipitation - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Immunoprecipitation - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    Histone H3 (citrulline R8) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate with Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] ab219406 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] ab219406 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate 10 μg (Input).
    Lane 2: Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] ab219406 IP in HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] ab219406 in HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] ab219406)

    Predicted band size: 15 kDa

    Exposure time: 1s

  • Flow Cytometry (Intracellular) - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Flow Cytometry (Intracellular) - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) transfected with empty vector (left panel) or PADI4 (WT, right panel), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, labeling Histone H3 (citrulline R8) with Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] ab219406 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 647) at 1/2000 dilution.

    Positive signal is obtained from HEK-293T cells transfected with WT PADI4 treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours.

  • Immunoprecipitation - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Immunoprecipitation - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    Histone H3 (citrulline R17) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate with Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] ab219407 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] ab219407 at 1/1000 dilution.

    VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate 10 μg (Input).
    Lane 2: Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] ab219407 IP in HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] ab219407 in HEK-293T transfected with PADI4 (WT), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] ab219407)

    Predicted band size: 15 kDa

    Exposure time: 1s

  • Flow Cytometry (Intracellular) - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Flow Cytometry (Intracellular) - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) transfected with empty vector (left panel) or PADI4 (WT, right panel), then treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours, labeling Histone H3 (citrulline R17) with Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] ab219407 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 647) at 1/2000 dilution.

    Positive signal is obtained from HEK-293T cells transfected with WT PADI4 treated with 10mM CaCl2 and 10μM Ionomycin (ab120116) for 2 hours.

  • Functional Studies - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Functional Studies - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    ab58668 staining ATF3 in A549 cells treated with ionomycin Ca2+ salt (ab120116), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin Ca2+ salt, as described in literature.
    The cells were incubated at 37°C for 2h in media containing different concentrations of ab120116 (ionomycin Ca2+ salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • Sandwich ELISA - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Sandwich ELISA - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    Sandwich ELISA - IFN gamma Human ELISA Kit (Human IFN gamma ELISA Kit ab46025)

    Jurkat were stimulated for 48 hours with 50 ng x mL-1 of PMA (Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) and 1 uM Ionomycin (ab120116) and PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Cell free supernatants were tested, showing results after background signal was subtracted (duplicates +/- SD).

  • Western blot - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116), expandable thumbnail

    Western blot - Ionomycin Ca2+ Salt, Ca2+ ionophore (ab120116)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:23129404 and PMID: 6423641).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Interferon gamma antibody [RM1245] (Anti-Interferon gamma antibody [RM1245] ab322926) at 1/1000 dilution

    Lane 1: Untreated No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) whole cell lysate at 20 µg

    Lane 2: No-GFP-CD16.NK-92 treated with 80nM PMA(TPA) (Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) and 3uM Ionomycin (ab120116) for 5 hours, 300 ng/ml BFA was added for last 4 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 17 kDa, 19 kDa, 23 kDa, 36 kDa

    Exposure time: 92s

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