MW 709 Da, Purity >97%. Potent, highly selective Ca2+ ionophore. Effective mobile ion carrier for Ca2+, highly specific for divalent cations (Ca > Mg >> Sr = Ba). Useful for studies of Ca2+ transport across biological membranes and increasing intracellular Ca2+ concentrations. Complexes with Ca2+ between pH 7 and 9.5. Absorption max. (280 nm, MeOH): 18600 M-1cm-1.
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- eLife 11:2022Identification of HIV-reservoir cells with reduced susceptibility to antibody-dependent immune response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntonio Astorga-Gamaza,Judith Grau-Expósito,Joaquín Burgos,Jordi Navarro,Adrià Curran,Bibiana Planas,Paula Suanzes,Vicenç Falcó,Meritxell Genescà,Maria J BuzonPubMed 35616530
- The Journal of general physiology 154:2022X-ray irradiation triggers immune response in human T-lymphocytes via store-operated Ca2+ entry and NFAT activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDominique Tandl,Tim Sponagel,Dalia Alansary,Sebastian Fuck,Timo Smit,Stephanie Hehlgans,Burkhard Jakob,Claudia Fournier,Barbara A Niemeyer,Franz Rödel,Bastian Roth,Anna Moroni,Gerhard ThielPubMed 35416945
- Frontiers in immunology 12:8133002022The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarla Usai,Joseph M Gibbons,Corinna Pade,Wenhao Li,Sabina R M Jacobs,Áine McKnight,Patrick T F Kennedy,Upkar S GillPubMed 35095908
- Immunity 54:1304-1319.e92021SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiao Lu,Jia Liu,Shuai Zhao,Maria Florencia Gomez Castro,Maudry Laurent-Rolle,Jianbo Dong,Xiaojuan Ran,Payal Damani-Yokota,Hongzhen Tang,Triantafyllia Karakousi,Juhee Son,Maria E Kaczmarek,Ze Zhang,Stephen T Yeung,Broc T McCune,Rita E Chen,Fei Tang,Xianwen Ren,Xufeng Chen,Jack C C Hsu,Marianna Teplova,Betty Huang,Haijing Deng,Zhilin Long,Tenny Mudianto,Shumin Jin,Peng Lin,Jasper Du,Ruochen Zang,Tina Tianjiao Su,Alberto Herrera,Ming Zhou,Renhong Yan,Jia Cui,James Zhu,Qiang Zhou,Tao Wang,Jianzhu Ma,Sergei B Koralov,Zemin Zhang,Iannis Aifantis,Leopoldo N Segal,Michael S Diamond,Kamal M Khanna,Kenneth A Stapleford,Peter Cresswell,Yue Liu,Siyuan Ding,Qi Xie,Jun WangPubMed 34048708
- The Journal of biological chemistry 295:10138-101522020Intramitochondrial proteostasis is directly coupled to α-synuclein and amyloid β1-42 pathologies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanin Lautenschläger et. al.PubMed 32385113
- Theranostics 10:5225-52412020Parthenolide ameliorates colon inflammation through regulating Treg/Th17 balance in a gut microbiota-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao-Jiang Liu et. al.PubMed 32373209
- mBio 8:2017A Novel Single-Cell FISH-Flow Assay Identifies Effector Memory CD4 T cells as a Major Niche for HIV-1 Transcription in HIV-Infected Patients.Applications:Unspecified applicationReactive species:HumanJudith Grau-Expósito,Carla Serra-Peinado,Lucia Miguel,Jordi Navarro,Adrià Curran,Joaquin Burgos,Imma Ocaña,Esteban Ribera,Ariadna Torrella,Bibiana Planas,Rosa Badía,Josep Castellví,Vicenç Falcó,Manuel Crespo,Maria J BuzonPubMed 28698276
- The Journal of neuroscience : the official journal of the Society for Neuroscience 32:11356-642012Activity-dependent cleavage of the K-Cl cotransporter KCC2 mediated by calcium-activated protease calpain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin Puskarjov,Faraz Ahmad,Kai Kaila,Peter BlaessePubMed 22895718
- Molecular and biochemical parasitology 179:59-682011Ca2+-activated transbilayer movement of plasma membrane phospholipids in Leishmania donovani during ionomycin or thapsigargin stimulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrien Weingärtner et. al.PubMed 21684309
Key facts
- CAS number
- 56092-81-0
- Purity
- > 97%
- Form
- Liquid
- Source
- Streptomyces conglobatus
- Molecular weight
- 709 Da
- Molecular formula
- C41H72O9
- PubChem identifier
- 6912226
- Nature
- Native
Target data
Recommended products
MW 709 Da, Purity >97%. Potent, highly selective Ca2+ ionophore. Effective mobile ion carrier for Ca2+, highly specific for divalent cations (Ca > Mg >> Sr = Ba). Useful for studies of Ca2+ transport across biological membranes and increasing intracellular Ca2+ concentrations. Complexes with Ca2+ between pH 7 and 9.5. Absorption max. (280 nm, MeOH): 18600 M-1cm-1.
Key facts
- CAS number
- 56092-81-0
- Purity
- > 97%
- Form
- Liquid
- Source
- Streptomyces conglobatus
- Molecular weight
- 709 Da
- Molecular formula
- C41H72O9
- PubChem identifier
- 6912226
- Nature
- Native
- Biochemical name
- Ionomycin
- Biological description
Potent, highly selective Ca2+ ionophore. Effective mobile ion carrier for Ca2+, highly specific for divalent cations (Ca > Mg >> Sr = Ba). Useful for studies of Ca2+ transport across biological membranes and increasing intracellular Ca2+ concentrations. Complexes with Ca2+ between pH 7 and 9.5. Absorption max. (280 nm, MeOH): 18600 M-1cm-1.
- Canonical SMILES
- CC(CCC(=O)O)CC(C)CC(C)C(=O)C=C(C(C)CC(C)CC=CC(C)C(C(C)C(CC1CCC(O1)(C)C2CCC(O2)(C)C(C)O)O)O)O
- Isomeric SMILES
- C[C@H](CCC(=O)O)C[C@H](C)C[C@H](C)C(=O)/C=C(/[C@H](C)C[C@H](C)C/C=C/[C@@H](C)[C@H]([C@@H](C)[C@H](C[C@@H]1CC[C@@](O1)(C)[C@H]2CC[C@@](O2)(C)[C@@H](C)O)O)O)\O
- InChI
- InChI=1S/C41H72O9/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47)/b13-11+,34-24-/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+/m1/s1
- InChIKey
- PGHMRUGBZOYCAA-ADZNBVRBSA-N
- IUPAC name
- (4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-trihydroxy-22-[(2S,5S)-5-[(2R,5S)-5-[(1R)-1-hydroxyethyl]-5-methyloxolan-2-yl]-5-methyloxolan-2-yl]-4,6,8,12,14,18,20-heptamethyl-9-oxodocosa-10,16-dienoic acid
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
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2 product images
Chemical Structure - Ionomycin (free acid), Ca2+ ionophore (ab120370)
2D chemical structure image of ab120370, Ionomycin (free acid), Ca2+ ionophore
Functional Studies - Ionomycin (free acid), Ca2+ ionophore (ab120370)
ab58668 staining ATF3 in A549 cells treated with ionomycin (free acid) (ab120370), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin (free acid), as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120370 (Ionomycin (free acid)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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