Itraconazole, Cytochrome p450 inhibitor

• FuncS

Unknown

Functional Studies - Itraconazole, Cytochrome p450 inhibitor (AB120816)

HepG2 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of itraconazole (ab120816). Increased expression of cytochrome P450 1A1 (ab3568) in HepG2 cells correlates with an increase in nifuroxazide concentration, as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3568 at 1/500 dilution and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

• Chemical Structure

Lab

Chemical Structure - Itraconazole, Cytochrome p450 inhibitor (AB120816)

2D chemical structure image of ab120816, Itraconazole, Cytochrome p450 inhibitor