MW 425.61 g/mol, Purity >98%. Smoothened inhibitor (IC50 = 500-700 nM). Steroidal alkaloid. Prevents hedgehog signaling and therefore Gli1 transcription.
Images
Key facts
- CAS number
- 469-59-0
- Purity
- > 98%
- Form
- Solid
- Source
- Veratrum nigrum L.
- Molecular weight
- 425.61 g/mol
- Molecular formula
- C27H39NO3
- Nature
- Native
Recommended products
MW 425.61 g/mol, Purity >98%. Smoothened inhibitor (IC50 = 500-700 nM). Steroidal alkaloid. Prevents hedgehog signaling and therefore Gli1 transcription.
Key facts
- CAS number
- 469-59-0
- Purity
- > 98%
- Form
- Solid
- Source
- Veratrum nigrum L.
- Molecular weight
- 425.61 g/mol
- Molecular formula
- C27H39NO3
- Nature
- Native
- Solubility
Soluble in DMSO to 10 mM.
Soluble in ethanol to 10 mM.
- Biological description
Smoothened inhibitor (IC50 = 500-700 nM). Steroidal alkaloid. Prevents hedgehog signaling and therefore Gli1 transcription.
- IUPAC name
- (3β,23β)-17,23-Epoxy-3-hydroxyveratraman-11-one
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Chemical Structure - Jervine, Hedgehog (Hh) signaling inhibitor (ab120815)
2D chemical structure image of ab120815, Jervine, Hedgehog (Hh) signaling inhibitor
Functional Studies - Jervine, Hedgehog (Hh) signaling inhibitor (ab120815)
NIH-3T3 cells were incubated at 37°C for 7h with vehicle control (0 μM) and 5 μM of jervine (ab120815) in DMSO. Decreased expression of smoothened (ab38686) in NIH-3T3 cells correlates with an increase in jervine concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab38686 at 1 μg /ml and Anti-beta Actin antibody - Loading Control ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution and visualised using ECL development solution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com