L-Glutamate, excitatory neurotransmitter

2 product images

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  Chemical Structure - L-Glutamate, excitatory neurotransmitter (ab120049)

  2D chemical structure image of ab120049, L-Glutamate, excitatory neurotransmitter

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  Immunocytochemistry/ Immunofluorescence - L-Glutamate, excitatory neurotransmitter (ab120049)

  Anti-GABA B Receptor 1 antibody [2D7] ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with L-Glutamate (ab120049), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of L-Glutamate, as described in literature.
  The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120049 (L-Glutamate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-GABA B Receptor 1 antibody [2D7] ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.