Skip to main content

Maleimide-Alkaline Phosphatase. Achieve your results faster with highly validated, pure and trusted compounds.

Be the first to review this product! Submit a review

Images

ELISA - Maleimide-Alkaline Phosphatase (AB270022), expandable thumbnail

Key facts

Form
Lyophilized

Recommended products

Maleimide-Alkaline Phosphatase. Achieve your results faster with highly validated, pure and trusted compounds.

Key facts

Storage

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes

Maleimide-Activated Alkaline Phosphatase (ab270022) is designed to facilitate the conjugation of Alkaline Phosphatase to proteins, peptides and ligands that contain sulfhydryl (-SH) groups. The reactive maleimide group that has been added to Alkaline Phosphatase allows conjugation to be performed very efficiently at physiological pH.

This product is manufactured by Expedeon, an Abcam company. It was previously called Maleimide-Alkaline Phosphatase. Expedeon product code 402-0002 is the same as the 2 mg size of this product.

The buffer in which the thiolated molecule is dissolved should be in the pH range 6.5-7.5. Phosphate buffered saline pH 7.2 is commonly employed.

Please see the below table for recommended buffer conditions and components:

Buffer components Compatible
pH range 6.5-7.5 Yes
Chelating agents (e.g. EDTA) No
Primary amines Yes
Reducing reagents No
Sodium Azide <0.1%

Molecules that do not contain –SH groups can be chemically modified to introduce the required thiol functionality.

Abcam offers a thiolation kit (ab270020) compatible with this product.

In the case of antibodies, disulfide (S-S) bridges may also be cleaved to release free thiols. Any reducing reagent (e. g. dithiothreitol and β-mercaptoethanol) used to produce free thiols should be removed before proceeding with the conjugation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • ELISA - Maleimide-Alkaline Phosphatase (ab270022), expandable thumbnail

    ELISA - Maleimide-Alkaline Phosphatase (ab270022)

    Thiolated goat anti-rabbit IgG was incubated with maleimideactivated Alkaline Phosphatase. The resulting conjugate was analysed by ELISA using a rabbit IgG coated plate and p-Nitrophenyl Phosphate (PNPP) as enzyme substrate.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com