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Maleimide-R Phycoerythrin. Achieve your results faster with highly validated, pure and trusted compounds.

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ELISA - Maleimide-R Phycoerythrin (AB270023), expandable thumbnail

Key facts

Form
Lyophilized

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Maleimide-R Phycoerythrin. Achieve your results faster with highly validated, pure and trusted compounds.

Key facts

Storage

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes

Maleimide-Activated R Phycoerythrin (ab270023) is designed to facilitate the conjugation of R-Phycoerythrin to proteins, peptides and ligands that contain sulfhydryl (-SH) groups. The reactive maleimide group that has been added to R-Phycoerythrin allows conjugation to be performed very efficiently at physiological pH.

This product is manufactured by Expedeon, an Abcam company. It was previously called Maleimide-R Phycoerythrin (2mg)SKU: 403-0002. Expedeon product code 403-0002 is the same as the 2 mg size of this product.

The buffer in which the thiolated molecule is dissolved should be in the pH range 6.5-7.5. Phosphate buffered saline pH 7.2 is commonly employed. EDTA (1-10 mM) may help to prevent metal catalyzed oxidation of thiols.

Please see the below table for recommended buffer conditions and components:

Buffer components Compatible
pH range 6.5-7.5 Yes
Chelating agents (e.g. EDTA) Yes
Primary amines Yes
Reducing reagents No
Sodium Azide <0.1%

Molecules that do not contain –SH groups can be chemically modified to introduce the required thiol functionality.

Abcam offers a thiolation kit (ab270020) compatible with this product.

In the case of antibodies, disulfide (S-S) bridges may also be cleaved to release free thiols. Any reducing reagent (e. g. dithiothreitol and β-mercaptoethanol) used to produce free thiols should be removed before proceeding with the conjugation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • ELISA - Maleimide-R Phycoerythrin (ab270023), expandable thumbnail

    ELISA - Maleimide-R Phycoerythrin (ab270023)

    Thiolated goat anti-rabbit IgG was incubated with maleimideactivated R-PE. The resulting conjugate was analyzed by ELISA using a rabbit IgG coated plate.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com