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AB144566

Methylstat, jumonji C domain-containing histone demethylase inhibitor

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(1 Publication)

MW 505.6 Da, Purity >90%. Selective Jumonji C domain-containing histone demethylase inhibitor. Anticancer agent, inhibits esophageal carcinoma cell growth (GI50 = 5.1 μM). Cell-permeable. Active in vivo and in vitro.
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Functional Studies - Methylstat, jumonji C domain-containing histone demethylase inhibitor (AB144566)
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Functional Studies - Methylstat, jumonji C domain-containing histone demethylase inhibitor (AB144566)

ab9050 staining Histone H3 (tri methyl K36) in MCF-7 cells. The cells were incubated with 15µM Methylstat (ab144566) for 24 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9050 at 0.1µg/ml and ab195889 Mouse monoclonal [DM1A] to alpha Tubulin - Alexa Fluor® 594 at 2µg/ml (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 1/1000 dilution (shown in green). Nuclear DNA was labelled in blue with DAPI. Negative controls : 1– anti-rabbit secondary only (solvent only); 2 – anti-rabbit secondary only (Treated). The secondary antibody control demonstrates that the labelling observed is due only to the binding of the secondary antibody to the primary antibody

Chemical Structure - Methylstat, jumonji C domain-containing histone demethylase inhibitor (AB144566)
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Chemical Structure - Methylstat, jumonji C domain-containing histone demethylase inhibitor (AB144566)

2D chemical structure image of ab144566, Methylstat, jumonji C domain-containing histone demethylase inhibitor

Key facts

CAS number

1310877-95-2

Purity

>90%

Form

Solid

form

Molecular weight

505.6 Da

Molecular formula

C<sub>2</sub><sub>8</sub>H<sub>3</sub><sub>1</sub>N<sub>3</sub>O<sub>6</sub>

PubChem

53392493

Nature

Synthetic

Solubility

Soluble in DMSO to 100 mM

Biochemical name

Methylstat

Biological description

Selective Jumonji C domain-containing histone demethylase inhibitor. Anticancer agent, inhibits esophageal carcinoma cell growth (GI50 = 5.1 μM). Cell-permeable. Active in vivo and in vitro.

Canonical smiles

COC(=O)C=CC(=O)N(CCCCNCC1=CC=C(C=C1)COC(=O)NC2=CC=CC3=CC=CC=C32)O

Isomeric smiles

COC(=O)/C=C/C(=O)N(CCCCNCC1=CC=C(C=C1)COC(=O)NC2=CC=CC3=CC=CC=C32)O

InChi

InChI=1S/C28H31N3O6/c1-36-27(33)16-15-26(32)31(35)18-5-4-17-29-19-21-11-13-22(14-12-21)20-37-28(34)30-25-10-6-8-23-7-2-3-9-24(23)25/h2-3,6-16,29,35H,4-5,17-20H2,1H3,(H,30,34)/b16-15+

InChiKey

MUJOCHRZXRZONW-FOCLMDBBSA-N

IUPAC Name

methyl (E)-4-[hydroxy-[4-[[4-(naphthalen-1-ylcarbamoyloxymethyl)phenyl]methylamino]butyl]amino]-4-oxobut-2-enoate

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Complementary Products

Biochemicals

AB144396

GSK-J1, H3K27 histone demethylase inhibitor

Chemical Structure - GSK-J1, H3K27 histone demethylase inhibitor (AB144396)

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Biochemicals

AB144534

Chaetocin, histone lysine methyltransferase inhibitor

Chemical Structure - Chaetocin, histone lysine methyltransferase inhibitor (AB144534)

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Primary Antibodies

AB9050

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade

ChIP - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

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Biochemicals

AB144621

Daminozide, histone demethylase KDM2/7 subfamily inhibitor

Chemical Structure - Daminozide, histone demethylase KDM2/7 subfamily inhibitor (AB144621)

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
It is important to note that this product is reported to be light sensitive|Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 215:2833-2849 PubMed30266800

2018

KDM4B-regulated unfolded protein response as a therapeutic vulnerability in -deficient breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Wang,Gokce Oguz,Puay Leng Lee,Yi Bao,Panpan Wang,Mikkel Green Terp,Henrik J Ditzel,Qiang Yu
View all publications
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