Skip to main content

MW 505.6 Da, Purity >90%. Selective Jumonji C domain-containing histone demethylase inhibitor. Anticancer agent, inhibits esophageal carcinoma cell growth (GI50 = 5.1 μM). Cell-permeable. Active in vivo and in vitro.

Be the first to review this product! Submit a review

Images

Chemical Structure - Methylstat, jumonji C domain-containing histone demethylase inhibitor (AB144566), expandable thumbnail
  • Functional Studies - Methylstat, jumonji C domain-containing histone demethylase inhibitor (AB144566), expandable thumbnail

Publications

Key facts

CAS number
1310877-95-2
Purity
> 90%
Form
Solid
Molecular weight
505.6 Da
Molecular formula
C28H31N3O6
PubChem identifier
53392493
Nature
Synthetic

Target data

Recommended products

MW 505.6 Da, Purity >90%. Selective Jumonji C domain-containing histone demethylase inhibitor. Anticancer agent, inhibits esophageal carcinoma cell growth (GI50 = 5.1 μM). Cell-permeable. Active in vivo and in vitro.

Key facts

Purity
> 90%
PubChem identifier
53392493
Solubility

Soluble in DMSO to 100 mM.

Biochemical name
Methylstat
Biological description

Selective Jumonji C domain-containing histone demethylase inhibitor. Anticancer agent, inhibits esophageal carcinoma cell growth (GI50 = 5.1 μM). Cell-permeable. Active in vivo and in vitro.

Canonical SMILES
COC(=O)C=CC(=O)N(CCCCNCC1=CC=C(C=C1)COC(=O)NC2=CC=CC3=CC=CC=C32)O
Isomeric SMILES
COC(=O)/C=C/C(=O)N(CCCCNCC1=CC=C(C=C1)COC(=O)NC2=CC=CC3=CC=CC=C32)O
InChI
InChI=1S/C28H31N3O6/c1-36-27(33)16-15-26(32)31(35)18-5-4-17-29-19-21-11-13-22(14-12-21)20-37-28(34)30-25-10-6-8-23-7-2-3-9-24(23)25/h2-3,6-16,29,35H,4-5,17-20H2,1H3,(H,30,34)/b16-15+
InChIKey
MUJOCHRZXRZONW-FOCLMDBBSA-N
IUPAC name
methyl (E)-4-[hydroxy-[4-[[4-(naphthalen-1-ylcarbamoyloxymethyl)phenyl]methylamino]butyl]amino]-4-oxobut-2-enoate

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
It is important to note that this product is reported to be light sensitive, Store under desiccating conditions, The product can be stored for up to 12 months

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Chemical Structure - Methylstat, jumonji C domain-containing histone demethylase inhibitor (ab144566), expandable thumbnail

    Chemical Structure - Methylstat, jumonji C domain-containing histone demethylase inhibitor (ab144566)

    2D chemical structure image of ab144566, Methylstat, jumonji C domain-containing histone demethylase inhibitor

  • Functional Studies - Methylstat, jumonji C domain-containing histone demethylase inhibitor (ab144566), expandable thumbnail

    Functional Studies - Methylstat, jumonji C domain-containing histone demethylase inhibitor (ab144566)

    Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade ab9050 staining Histone H3 (tri methyl K36) in MCF-7 cells. The cells were incubated with 15µM Methylstat (ab144566) for 24 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade ab9050 at 0.1µg/ml and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Mouse monoclonal [DM1A] to alpha Tubulin - Alexa Fluor® 594 at 2µg/ml (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution (shown in green). Nuclear DNA was labelled in blue with DAPI.
    Negative controls: 1– anti-rabbit secondary only (solvent only); 2 – anti-rabbit secondary only (Treated). The secondary antibody control demonstrates that the labelling observed is due only to the binding of the secondary antibody to the primary antibody

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com