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AB120466

MiTMAB™, dynamin I and dynamin II inhibitor

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(11 Publications)

MW 336.4 Da. Cell-permeable dynamin I and dynamin II inhibitor (IC50 values are 3.1 and 8.4 µM for inhibition of dynamin I and dynamin II GTPase, respectively). Targets the pleckstrin homology (PH) (lipid binding) domain. Competitive with phospholipid and non-competitive with GTP. Inhibits receptor-mediated and synaptic vesicle endocytosis (IC50 values are 19.9 and 2.2 µM, respectively). Inhibits cancer cell growth.
2 Images
Immunocytochemistry/ Immunofluorescence - MiTMAB™, dynamin I and dynamin II inhibitor (AB120466)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - MiTMAB™, dynamin I and dynamin II inhibitor (AB120466)

ab66705 staining PAI1 in HeLa cells treated with MiTMAB™ (ab120466), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of MiTMAB™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120466 (MiTMAB™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Chemical Structure - MiTMAB™, dynamin I and dynamin II inhibitor (AB120466)
  • Chemical Structure

Lab

Chemical Structure - MiTMAB™, dynamin I and dynamin II inhibitor (AB120466)

2D chemical structure image of ab120466, MiTMAB™, dynamin I and dynamin II inhibitor

Key facts

CAS number

1119-97-7

Form

Solid

form

Molecular weight

336.4 Da

Molecular formula

C<sub>1</sub><sub>7</sub>H<sub>3</sub><sub>8</sub>BrN

PubChem

14250

Nature

Synthetic

Solubility

Soluble in water to 100 mM

Biochemical name

Cetrimide

Biological description

Cell-permeable dynamin I and dynamin II inhibitor (IC50 values are 3.1 and 8.4 µM for inhibition of dynamin I and dynamin II GTPase, respectively). Targets the pleckstrin homology (PH) (lipid binding) domain. Competitive with phospholipid and non-competitive with GTP. Inhibits receptor-mediated and synaptic vesicle endocytosis (IC50 values are 19.9 and 2.2 µM, respectively). Inhibits cancer cell growth.

Canonical smiles

CCCCCCCCCCCCCC[N+](C)(C)C.[Br-]

InChi

InChI=1S/C17H38N.BrH/c1-5-6-7-8-9-10-11-12-13-14-15-16-17-18(2,3)4;/h5-17H2,1-4H3;1H/q+1;/p-1

InChiKey

CXRFDZFCGOPDTD-UHFFFAOYSA-M

IUPAC Name

trimethyl(tetradecyl)azanium;bromide

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Biochemicals

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Immunocytochemistry/ Immunofluorescence - Dynole® 34-2, dynamin I and dynamin II inhibitor (AB120463)

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Biochemicals

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Product details

Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. MiTMAB™ and OcTMAB™ are trademarks of Children's Medical Research Institute and Newcastle Innovation Ltd.
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dynamin 1 also known as DNM1 is a GTPase with a molecular mass of approximately 96 kDa. It plays a mechanical role in membrane fission during endocytosis. Dynamin 1 is primarily expressed in neuronal tissues where it participates in the formation and release of synaptic vesicles. Its ability to hydrolyze GTP gives it the mechanical force needed to constrict and sever membranes which is vital for efficient synaptic vesicle recycling in neurons.
Biological function summary

Dynamin 1 helps regulate synaptic transmission by controlling vesicular traffic in neurons. As part of a larger protein complex it interacts with various proteins to mediate endocytosis specifically in presynaptic nerve terminals. These interactions ensure that synaptic vesicles are recycled rapidly allowing neurons to maintain communication across synapses. Disruptions in dynamin 1 function can impair synaptic vesicle recycling affecting neurotransmission efficiency.

Pathways

Dynamin 1 is essential in the clathrin-mediated endocytic pathway. This pathway is a major route for internalizing synaptic vesicles and involves cooperation with other proteins such as clathrin and adaptor proteins. Additionally dynamin 1 participates in the actin cytoskeleton dynamics linking it with actin-associated proteins. These pathways highlight its contributions to maintaining synaptic vesicle endocytosis and neurite outgrowth processes.

Alterations in dynamin 1 function are linked to neurological disorders including epilepsy and Huntington's disease. In epilepsy improper functioning of dynamin 1 disrupts synaptic vesicle recycling leading to imbalances in neurotransmitter release. Connections exist between dynamin 1 and huntingtin where their interactions in vesicular trafficking are important for neuronal stability. Malfunctions in these processes often result in neurodegenerative symptoms emphasizing dynamin 1's involvement in sustaining neural health.
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Product protocols

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39508547

2024

Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongsik Kim,Dahyun Kim,Dong-Kyun Kim,Sang-Hee Lee,Wonyul Jang,Dae-Sik Lim

International journal of molecular sciences 24: PubMed37240384

2023

ATP Consumption Is Coupled with Endocytosis in Exudated Neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Duo Wang,Zirui Zeng,Mengyue Shen,Ryuji Okazaki,Hironori Miyata,Tomo Yonezawa,Yasuhiro Yoshida

Life (Basel, Switzerland) 11: PubMed34575026

2021

Dynamin Inhibitors Prevent the Establishment of the Cytomegalovirus Assembly Compartment in the Early Phase of Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Igor Štimac,Natalia Jug Vučko,Gordana Blagojević Zagorac,Marina Marcelić,Hana Mahmutefendić Lučin,Pero Lučin

Tissue barriers 9:1929786 PubMed34107845

2021

Asymmetric distribution of dynamin-2 and β-catenin relative to tight junction spikes in alveolar epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

K Sabrina Lynn,Kristen F Easley,Francisco J Martinez,Ryan C Reed,Barbara Schlingmann,Michael Koval

International immunopharmacology 84:106510 PubMed32361568

2020

ERK is involved in the differentiation and function of dimethyl sulfoxide-induced HL-60 neutrophil-like cells, which mimic inflammatory neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Duo Wang,Yusuke Sennari,Mengyue Shen,Kentaro Morita,Tamotsu Kanazawa,Yasuhiro Yoshida

Science signaling 13: PubMed32209697

2020

Noncanonical STAT1 phosphorylation expands its transcriptional activity into promoting LPS-induced IL-6 and IL-12p40 production.

Applications

Unspecified application

Species

Unspecified reactive species

Hozaifa Metwally,Toshio Tanaka,Songling Li,Gyanu Parajuli,Sujin Kang,Hamza Hanieh,Shigeru Hashimoto,Jaya P Chalise,Yohannes Gemechu,Daron M Standley,Tadamitsu Kishimoto

FEBS open bio 9:291-303 PubMed30761254

2019

Transferrin receptor 1 is required for enucleation of mouse erythroblasts during terminal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Mamoru Aoto,Akiho Iwashita,Kanako Mita,Nobutaka Ohkubo,Yoshihide Tsujimoto,Noriaki Mitsuda

The Journal of cell biology 217:2911-2929 PubMed29921601

2018

Septins suppress the release of vaccinia virus from infected cells.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Pfanzelter,Serge Mostowy,Michael Way

Nature communications 5:4886 PubMed25216363

2014

Dynamic catch of a Thy-1-α5β1+syndecan-4 trimolecular complex.

Applications

Unspecified application

Species

Unspecified reactive species

Vincent F Fiore,Lining Ju,Yunfeng Chen,Cheng Zhu,Thomas H Barker

The Journal of cell biology 205:721-35 PubMed24891602

2014

Actin and dynamin2 dynamics and interplay during clathrin-mediated endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandre Grassart,Aaron T Cheng,Sun Hae Hong,Fan Zhang,Nathan Zenzer,Yongmei Feng,David M Briner,Gregory D Davis,Dmitry Malkov,David G Drubin
View all publications
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